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Method for improving oncidium protocorm proliferation and differentiation by using concentrated coconut water

A technology of oncidium protospheres and coconut water, applied in horticultural methods, botanical equipment and methods, applications, etc., can solve the problems of high cost of coconut water, inconvenient transportation, large coconut fruit, etc., and improve the reproduction of tissue cultured seedlings rate, increase the proliferation rate, the effect of strong practicability

Inactive Publication Date: 2011-12-28
COCONUT RES INST OF CHINESE ACAD OF TROPICAL AGRI SCI
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Problems solved by technology

[0003] Due to the large amount of coconut water added in tissue culture, and at present, the source of coconut in my country is limited to Hainan Province, the production area is small, and the coconut fruit is large, and the transportation is quite inconvenient. Therefore, the cost of obtaining coconut water in tissue culture factories is relatively high. It is unfavorable for the application of coconut water in tissue culture, and restricts the production route of oncidium tissue culture, but the impact of concentrated coconut water on plant tissue culture process has not been reported yet

Method used

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  • Method for improving oncidium protocorm proliferation and differentiation by using concentrated coconut water
  • Method for improving oncidium protocorm proliferation and differentiation by using concentrated coconut water

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Embodiment 1

[0027] 1. Material: Oncidium concolor of the same color

[0028] 2. Disinfection of explants: In sunny weather, wrap the stem tip of Oncidium in the morning, take it back to the laboratory and rinse it with tap water quickly, leave the young shoots about 2-3cm long, and use sterile water on the ultra-clean workbench Rinse twice, then soak in 70% alcohol for 1 minute, pour off the alcohol and add 0.15% HgCl 2 The solution was sterilized for 8 minutes, rinsed with sterile water for 5 times, blotted dry with sterilized filter paper, stripped the shoot tip and inoculated it on the induction medium for induction culture, the induction medium was MS+6-BA 4mg / L+ NAA 0.5mg / L+30g / L sucrose+5.0g / L agar powder+1g / L activated carbon+1% volume coconut water; culture conditions are temperature 26°C, pH 5.8, light intensity 1500-2000lx, light 12 hours / day . After 15 days, the shoot tip began to swell, and after 23 days, a callus-like papillae appeared at the base of the shoot tip, and prot...

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Abstract

The invention discloses a method for improving the proliferation and differentiation of oncidium protocorms by using concentrated coconut water. The coconut water is placed on a rotary evaporator for concentration to obtain concentrated coconut water for subsequent use; Inoculate the protocorms produced by inducing culture on the induction medium added with concentrated coconut water after the bacteria, transfer the protocorms to the proliferation medium for proliferation culture, and then transfer to the differentiation medium for differentiation culture to produce clustered buds. The clustered shoots were inoculated on the medium for rooting and strong seedlings and cultivated until they became seedlings. The present invention uses concentrated coconut water as an organic additive to significantly increase the proliferation rate of oncidium protocorm tissue, and on the basis of increasing the proliferation rate of protocorm tissue, effectively improves the reproduction rate of tissue-cultured seedlings, providing a method for industrial production of oncidium Provide an effective way, strong practicability, simple operation, good popularization.

Description

technical field [0001] The invention relates to a protocorm proliferation and differentiation method of Oncidium orchid, in particular to a method for significantly improving the protocorm proliferation and differentiation of Oncidium by adding concentrated coconut water. Background technique [0002] Orchid is a kind of flower famous for its fragrance. With its unique leaves, flowers and fragrance, it has four cleans (clear air, clear color, clear spirit and clear rhyme) to give people a very pure and elegant image. It has a very high price and is known as a gentleman among flowers. The germination rate of orchid seeds is low, the time is long, and it is not neat, so that the cultivation and production of orchids cannot better meet the needs of the market. At present, the production of orchid seedlings mainly utilizes the technical method of tissue culture, which can effectively improve the rapid propagation coefficient of orchid varieties. Using the method of tissue cult...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
Inventor 黄丽云范海阔周焕起李杰覃伟权马子龙
Owner COCONUT RES INST OF CHINESE ACAD OF TROPICAL AGRI SCI
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