Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

A kind of oncidium rapid propagation medium

A technology for multiplying culture medium and culture medium, applied in plant cells, horticulture, botany equipment and methods, etc., can solve the problems of virus affecting plant growth and flower ornamental, unsatisfactory culture effect, and high transplant mortality, and achieves The effect of improving resistance and transplanting survival rate, improving tissue culture seedling resistance and transplanting survival rate, and enhancing the environmental adaptability of seedlings

Active Publication Date: 2016-08-24
INST OF DAFENG MARINE IND NANJING UNIV OF TECH
View PDF5 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Many oncidium varieties have been introduced into my country in recent years, but the production of seedlings mainly depends on ramets, which not only reproduces slowly, but also accumulates viruses that seriously affect the growth of plants and the ornamental quality of flowers.
The use of plant tissue culture technology can solve the problem of rapid propagation of oncidium high-quality seedlings. At present, there are reports and patents on oncidium tissue culture in China, but the culture effect is not ideal. The resistance of tissue culture seedlings is poor, and the transplanting mortality rate is high.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Propagation object: protocorms of oncidium explants

[0026] The cultivation process is divided into three stages, as follows:

[0027] The first stage: Cultivate protocorm proliferation medium, inoculate oncidium protocorm into the protocorm proliferation medium for culture, the medium formula is: MS+6-BA 3.0mg / L+NAA 0.25mg / L+coconut juice 100ml / L + sucrose 30g / L + agar 5.5g / L, pH 5.5; Proliferation culture 15d-25d, protocorm proliferation culture ends.

[0028] The second stage: strong seedling cultivation, the protocorm is connected to the strong seedling medium for cultivation, the medium formula is: MS+peptone 2.0g / L+banana 30g / L+potato 30g / L+sucrose 25g / L+agar 5.5g / L, PH 6.0; Strong seedlings are cultivated for 25d-35d, and the strong seedlings are cultivated.

[0029] The third stage: rooting culture, inoculate the single-plant tissue culture seedlings with a height of more than 1cm obtained from strong seedling cultivation into the rooting medium for cultivat...

Embodiment 2

[0033] Propagation object: protocorms of oncidium explants

[0034] The cultivation process is divided into three stages, as follows:

[0035] The first stage: protocorm proliferation medium, oncidium protocorm is inoculated into the protocorm proliferation medium for culture, the medium formula is: MS+6-BA 3.5mg / L+NAA 0.3mg / L+coconut milk 150ml / L + sucrose 35g / L + agar 5.5g / L, PH 5.8; Proliferation culture 15d-25d, protocorm proliferation culture stage ends;

[0036] The second stage: strong seedling cultivation, the protocorm is connected to the strong seedling medium for cultivation, the medium formula is: MS+peptone 2.5g / L+banana 35g / L+potato 35g / L+sucrose 30g / L+agar 5.5g / L, PH 6.0; The strong seedlings are cultivated for 30d-35d, and the single tissue cultured seedlings with a size of 1cm or more are obtained, and the strong seedlings are cultivated.

[0037] The third stage: rooting culture, inoculate the single plant tissue culture seedlings with a height of more tha...

Embodiment 3

[0041] Propagation object: protocorms of oncidium explants

[0042] The cultivation process is divided into three stages, as follows:

[0043]The first stage: protocorm proliferation medium, oncidium protocorm is inoculated into the protocorm proliferation medium for culture, the medium formula is: MS+6-BA 2.0mg / L+NAA 0.2mg / L+coconut milk 80ml / L+sucrose 25g / L+agar 5.5g / L, pH 5.5; Proliferation culture 18d-30d.

[0044] The second stage: strong seedling cultivation, after 18d-30d of proliferation culture, connect the protocorm to the strong seedling medium for cultivation, the medium formula is: MS+peptone 1.5g / L+banana 25g / L+potato 25g / L+sucrose 20g / L+ Agar 5.5g / L, pH 6.0; Strong seedlings were cultured for 30d-40d to obtain single tissue cultured seedlings with a height of more than 1cm.

[0045] The third stage: rooting culture, inoculate the single plant tissue culture seedlings obtained from the cultivation of strong seedlings with a height of 1cm or more into the rooti...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a rapid propagation medium for oncidium, which comprises a three-stage formula of protocorm proliferation medium, seedling growth medium and rooting medium. In about time, the oncidium protocorm of the explant can be transformed into a rooted oncidium with a higher survival rate for transplanting through three stages of protocorm value-added cultivation, strong seedling cultivation and rooting cultivation, improving the It improves the rapid breeding ability of oncidium high-quality seedlings, and enhances the environmental adaptability of oncidium seedlings, thereby improving the tissue culture seedling resistance and transplanting survival rate of oncidium, and its transplanting survival rate is over 97%. , so that Oncidium is widely used in China.

Description

technical field [0001] The invention relates to the technical field of plant tissue culture, in particular to an oncidium rapid propagation medium. Background technique [0002] Oncidium, also known as Dancing Girl Orchid, Dancing Orchid, Tumor Petal Orchid, belongs to the genus Oncidium of Orchidaceae. There are more than 400 native species in the genus. It is native to Mexico, West Indies, Brazil and other places. It has a wide distribution area, many varieties, and great differences in growth habits. It is one of the large genera of orchids. The variety is mostly golden yellow with brown spots. In the research, pink, purple, pure yellow, wine red and other excellent varieties with beautiful flowers and perennial flowering were bred through interspecific and intergeneric hybridization. Oncidium's special shape, straight and long pedicels, rich colors, and long flowering period, so as an important cut flower and potted flower species, the market demand has increased sharp...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): C12N5/04A01H4/00
Inventor 张本厚胡燕花陈集双
Owner INST OF DAFENG MARINE IND NANJING UNIV OF TECH
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com