Immunohistochemical double-molecular-target single staining kit as well as using method and application thereof

Abstract

The invention discloses an immunohistochemical double-molecular-target single staining kit as well as a using method and an application thereof. Based on an expression pattern of a molecular target ina sample to be detected, a cell core type molecular target and a cytoplasm or cell membrane or plasma membrane type molecular target are selected as the to-be-detected double-molecular targets; then,specific to the specific antibody of the double-molecular targets, a kit comprising a mixing primary antibody is formed by mixing, wherein the kit can be used in an immunohistochemical staining method such as an immuno-enzyme linked immunosorbent assay or an immune colloidal gold method; the kit and an enzyme labeled secondary antibody and a chromogenic substrate matched with the kit are adopted,so that synchronous detection of two molecular targets can be completed on one slice through one immunohistochemical staining process. The method is obviously superior to the traditional immunohistochemical and immunohistochemical double staining method, and has the characteristics of low cost and high efficiency, and does not need to increase the additional operation steps.

Description

technical field [0001] The invention belongs to the field of immunohistochemical staining, and relates to an immunohistochemical double-label single-staining kit and its use method and application. Background technique [0002] Immunohistochemistry (IHC), also known as immunocytochemical staining, or immunohistochemistry for short, is the most important platform technology for pathological diagnosis, differential diagnosis, screening and identification of tumor-specific molecular targets. It plays an important role in the detection of specific molecular targets before chemotherapy, and has been used as a companion diagnostic technology for the sensitivity assessment of various molecular targeted drugs. [0003] The development of immunohistochemical technology has a history of more than 80 years. Since Coons first successfully used fluorescein-labeled antibody to detect pneumococcus in 1941, immunohistochemical technology has been born. Since then, based on different techn...

AI Technical Summary

Problems solved by technology

However, the existing immunohistochemical double-staining technology not only requires two specific primary antibodies, two enzyme-labeled secondary antibodies, two chromogenic substrates, and two immunohistochemical staining procedures, but most of them also require special enhancing reagents to complete

In fact, such a cumbersome operation process severely limits its widespread use in clinical work

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