42results about How to "Suitable for high-throughput screening" patented technology

The invention provides a fluorescence polarization immunoassay method for detection of erythromycin. The method includes: subjecting an erythromycin standard substance solution with a known concentration to mixed incubation respectively with a fluorescent marker solution and an erythromycin monoclonal antibody solution to carry out competing reaction, thus obtaining a fluorescence polarization value of the system; taking the measured fluorescence polarization value as the ordinate, and adopting the concentration of a series of sulfamethazine standard substances with known concentration as the abscissa to draw a standard curve; using a to-be-measured sample to replace the erythromycin standard substance in step 1), according to the operation in step 1), determining the fluorescence polarization value of the system after incubation, and according to the standard curve, calculating the erythromycin concentration of the to-be-measured sample. The invention provides the homogeneous and rapid erythromycin fluorescence polarization immunodetection method, which only needs sample adding, has no need of separation and washing operation, and can obtain the detection result within 10min.

The invention relates to the production technology of spirulina, and aims to provide a method for identifying algal filament draining performance of spirulina by using a transmission electron microscope. The method comprises the following steps: extracting an algae solution of Spirulina platensis from a cultivation pool, selecting algal filament monomers with the lengths of greater than 300 microns by using a capillary pipet microscopic separation method, and cultivating the algal filament monomers into algal filament groups; sampling the obtained algal filament groups, and observing by usingthe transmission electron microscope; if polyphosphate particles inside algae cells are scattered and the maximum diameter is not greater than 0.2 micron, the identified algal filaments are of good draining performance; and if the polyphosphate particles inside algae cells are aggregated and the maximum diameter is greater than 0.4 microns, the identified algal filaments are of poor draining performance. According to the method, the algal filament draining performance of spirulina is identified based on the transmission electron microscope, and compared with a traditional method, the method issimple, convenient, efficient and low in cost, and is applicable for high-flux screening.

The invention provides a high-performance liquid detection and content determination method for ectoine. Chromatographic conditions are as follows: the chromatographic column adopts an adamantine-bonded chromatographic column; the detector adopts a DAD detector; the mobile phase comprises a mobile phase A and a mobile phase B; the mobile phase A is methanol; the mobile phase B is an aqueous buffersolution of monopotassium phosphate; and the elution mode adopts isocratic elution. By utilizing the chromatographic conditions, a test solution and a standard solution are injected into a liquid chromatograph, the chromatogram is recorded, the peak area is calculated according to an external standard method, and the content of ectoine in the sample can be accurately determined. The method disclosed by the invention is short in detection time, excellent in separation effect, high in precision and accuracy and low in cost and has an important application value in aspects of strain screening and related quantitative researches during ectoine production.

The invention discloses a high performance liquid detection method of dexamethasone, which adopts a reversed-phase C18 chromatographic column and a DAD detector, a mobile phase A is an aqueous solution of trifluoroacetic acid, a mobile phase B is an acetonitrile solution of trifluoroacetic acid, and gradient elution is adopted. By adopting the method disclosed by the invention to inject 3 to 5mu Lof samples, dexamethasone and related substances thereof can be effectively detected, the separation degree R can reach 1.5 or above, and an HPLC spectrum baseline is stable and free from drifting; the method is short in detection time, the high-performance liquid phase detection process can be completed within 3 min, the sample consumption is greatly reduced, meanwhile, the detection efficiencyis improved, and the method is suitable for high-throughput screening; the solvent can be saved and the cost is reduced; the method disclosed by the invention is used for measuring the content of dexamethasone, has a good linear relationship and high reproducibility, and has an important research value in the aspects of research on the quality of crude drugs and preparations, quantitative researchon related pharmacokinetics and the like.

The invention belongs to a technology for development and application of spirulina, and aims at providing a method for judging whether spirulina strains can be applied in large-scale aquaculture production. The method comprises the following steps of: carrying out sequencing on ftsZ genes of the identified spirulina strains, and carrying out variance analysis on site numbers and phylogenetic treeconstruction on the basis of a ftsZ gene sequence together with the known spirulina platensis; and if the identified spirulina strains and the ftsZ gene sequence of the known spirulina platensis strains are gathered into one type, showing that the production traits of the identified spirulina strains are good, and the identified spirulina strains can be applied in large-scale aquaculture production. Compared with the conventional method, the new method for identifying the good and bad production traits of the spirulina strains by applying the ftsZ gene sequence not only is simple, fast, standard and accurate, but also is low in cost, and is suitable for large-scale and high-throughput screening.

Provided are a cell model and screening method for screening inhibitors of the tubercle bacillus. Provided is a cell which expresses the tubercle bacillus proteins L12 and L10, wherein the L12 encoding gene and the transcription activation domain of its transcription factor are located in one expression vector, and the L10 encoding gene and the DNA binding domain of its transcription factor are located in another expression vector. The present invention also relates to the use of the compound shown in formulae I or II in preparing a blocker of the interaction between proteins L12 and L10 of tubercle bacillus, an anti-tubercle bacillus drug, or a drug for treating and / or preventing tuberculosis.

The invention discloses a method for detecting cyclic adenosine monophosphate in urine by liquid chromatography-mass spectrometry, which comprises the following steps: (1) sample pretreatment: the urine sample is pretreated by anion-exchange solid-phase extraction to obtain a test solution; (2) Adopt liquid chromatography-mass spectrometry to detect described need testing solution: chromatographic condition adopts reverse phase C18 hydrophilic chromatographic column; Adopt gradient elution; Mass spectrometry condition is: ion source is electrospray ion source; Ion mode is positive ion mode ; The monitoring mode is multiple reaction monitoring. The invention also discloses an internal standard quantitative detection method for detecting cyclic adenosine monophosphate in urine by liquid mass spectrometry. The method of the present invention is the first liquid chromatography tandem mass spectrometry detection method for cAMP in urine samples, which can effectively detect cAMP in urine samples, has good separation effect, stable baseline, short detection time, good linearity, and high accuracy. Good reproducibility, high sensitivity and high precision.

The invention discloses a quick detection method by using an interior label and taking a sigD / sigE gene of salmonella as a target through reverse transcription fluorescent quantitation PCR (Polymerase Chain Reaction). In the quick detection method, an RNA (Ribonucleic Acid) interior label obtained by PCR amplification for three times is composed. In addition, the invention further provides a corresponding kit and application. The method disclosed by the invention can be widely applied to the fields such as research of laboratories, food safety and health and hygiene. The phenomenon of false negative missed detection can be avoided, so that the PCR amplification is effectively monitored; in addition, the detection specificity of salmonella is strong, so that dead bacteria and live bacteria can be effectively distinguished; the quickness and high detection sensitivity are obtained; and the method can be applied to high-throughput screening of the salmonella in the food.

The invention discloses a method for preparation of a liver fibrosis animal model with juvenile zebrafish. The method adopts juvenile zebrafish as the model animal, and selects diethylnitrosamine andethanol as the modeling drug. The zebrafish genome adopted by the method provided by the invention is similar to the human genome, and presents physiological characteristics very similar to human, andcan be well applied to study of various human diseases. Also the method has the advantages of short modeling cycle, low cost and simple operation. The obtained model can be applied to high throughputscreening of various drugs.

The invention relates to a spirulina production technology, and aims at providing a method for identifying advantages and disadvantages of the spirulina filament drainage performance through RAPD detection. The method comprises the steps that spirulina platensis liquid is taken from a cultivating pool, an algal filament monomer with the length larger than or equal to 300 micrometer is picked through a capillary pipette micro-separation method, and an algal filament colony is cultivated; sampling is conducted on the obtained algal filament colony, and then random amplification polymorphic DNA analysis and detection are conducted; if a final 590 bp band cannot be amplified through a primer S90, it is indicated that the identified algal filaments have the good drainage performance; otherwise, it is indicated that the identified algal filaments is poor in drainage performance. According to the method, the advantages and disadvantages of the spirulina filament drainage performance are identified on the basis of a RAPA technology, compared with a conventional method, the method is simple, efficient, low in cost and suitable for high-throughput screening.

The invention discloses a method for screening monoclonal antibodies on different antigen binding sites. The method comprises the following steps: preparing an antigen specific monoclonal antibody by an antigen immune animal with two or more antigenic determinants through a hybridoma monoclonal technology; carrying out in-vitro cell culture on positive clone cell strains to obtain cell culture liquid supernatant containing the antigen specific monoclonal antibody; adding the hybridoma cell culture liquid supernatant into a solution of rubber latex grains which are covalently cross-linked with protein A or G in a two-and-two combined manner; and incubating and adding antigens to obtain a monoclonal antibody set with the increased light absorbance, namely obtain the monoclonal antibody with the different antigen binding sites.

The invention provides a method for detecting novel bacillus cereus. The detection method comprises the following steps: 1) extracting a deoxyribonucleic acid (DNA) in a to-be-detected sample; and 2) by taking the DNA obtained in the step 1) as a template, carrying out real-time fluorescent quantization polymerase chain reaction (PCR) detection, wherein the target for real-time fluorescent quantization PCR detection is a bacillus cereus murB gene. The invention also provides a kit for detecting bacillus cereus. The kit comprises a primer for amplification of the bacillus cereus murB gene.

The invention provides a cancer combined diagnosis marker comprising lncRNA H19 and lncRNA PTCSC3, and application of the cancer combined diagnosis marker in preparation of a reagent for cancer diagnosis, cancer prognosis evaluation or cancer treatment monitoring. The lncRNA H19 and the lncRNA PTCSC3, serving as the cancer combined diagnosis marker, have the advantages of high sensitivity, high specificity and accurate detection result, and can be applied to classification of cancer samples. The invention also provides a primer, a detection probe and a detection chip for cancer diagnosis, and a kit comprising the same. The expression contents of the lncRNA H19 and the lncRNA PTCSC3 in the samples are detected by using the primer, the detection probe, the detection chip or the kit respectively, lymph node metastasis of thyroid cancer can be effectively judged, and reliable information is provided for diagnosis, prognosis evaluation and curative effect monitoring of the thyroid cancer.

The invention relates to a spirulina development and application technology and aims to provide a method for distinguishing the degrees of production traits of a spirulina strain. The method provided by the invention comprises the following steps of: extracting water-soluble protein of spirulina cells with an tris-HCl extracting solution, separating a protein sample by carrying out sodium dodecyl sulphate-polyacrylamide gel electrophoresis, then detecting the light intensity value of a protein band at a 102kD site of a protein electrophoretogram, and constructing a hierarchical diagram among algae plants according to the light intensity value; if the light intensity value of the protein band of a candidate strain at the 102kD site is more than 140 and the candidate strain and a known strain with good production traits get together, indicating that the candidate strain has good production traits and applicable to large-scale cultivation production; and if the light intensity value of the protein band of the candidate strain at the 102kD site is less than 40 and the candidate strain and a known strain with poor production traits get together, indicating that the candidate strain has bad production traits and is not applicable to large-scale cultivation production. The method provided by the invention is simple, efficient, reliable and low in cost, and no complex test and analysis such as nucleic acid sequencing and bioinformatics comparison is carried out, so that the method provided by the invention is applicable to large-scale high throughput screening.

The invention relates to a spirulina production technology, and aims to provide a method for identifying the draining performance of spirulina filaments by utilizing a scanning electron microscope. The method comprises the following steps: extracting spirulina liquid of spirulina platensis from a cultivation pool, selecting spirulina filament monomers with the lengths larger than or equal to 300 [mu]m by using a capillary pipet microseparation method, and cultivating the spirulina filament monomers into a spirulina filament group; sampling the obtained spirulina filament group, and observing the sampled spirulina filament group by using the scanning electron microscope; if no milky white adhesive substance exists between the spirulina filaments, indicating that the identified spirulina filaments have good draining performance; and if the milky white adhesive substance exists between the spirulina filaments, indicating that the draining performance of the identified spirulina filaments is poor. Compared with a traditional method, the method for identifying the draining performance of the spirulina filaments based on the scanning electron microscope technology is simple and convenient, efficient and low in cost, and is suitable for high-throughput screening.