A method for identifying the advantages and disadvantages of spirulina algae silk drainage performance by rapd detection

Abstract

The invention relates to a spirulina production technology, and aims at providing a method for identifying advantages and disadvantages of the spirulina filament drainage performance through RAPD detection. The method comprises the steps that spirulina platensis liquid is taken from a cultivating pool, an algal filament monomer with the length larger than or equal to 300 micrometer is picked through a capillary pipette micro-separation method, and an algal filament colony is cultivated; sampling is conducted on the obtained algal filament colony, and then random amplification polymorphic DNA analysis and detection are conducted; if a final 590 bp band cannot be amplified through a primer S90, it is indicated that the identified algal filaments have the good drainage performance; otherwise, it is indicated that the identified algal filaments is poor in drainage performance. According to the method, the advantages and disadvantages of the spirulina filament drainage performance are identified on the basis of a RAPA technology, compared with a conventional method, the method is simple, efficient, low in cost and suitable for high-throughput screening.

Description

technical field [0001] The invention belongs to the production technology of spirulina, and in particular relates to a method for identifying whether the draining performance of spirulina algal filaments is good or bad through RAPD detection. Background technique [0002] Spirulina is a kind of prokaryotic filamentous microalgae with photosynthetic oxygen release and regular spiral, which is a genus of Cyanophyta, Oscillatoriales and Oscillatoriaceae [Wuhan Botany Research, 1997, 15(4):369-374]. Because it is rich in high-quality protein and a variety of biologically active substances, it has attracted great attention at home and abroad, and has formed a huge Spirulina industry on the basis of a large number of researches. It has become the world's largest research and development scale and the most extensive application prospects. microalgae. At present, the production of spirulina powder at home and abroad is generally cultivated in a circular racetrack pool, and then th...

AI Technical Summary

Problems solved by technology

Such a high drying energy consumption not only greatly limits the large-scale application of spirulina powder as a high-quality feed protein source in areas with greater demand such as livestock and poultry and aquaculture, but also some production bases burn coal or even burn coal without authorization to reduce drying costs. firewood, seriously destroying the ecology and polluting the environment

For this reason, for more than 20 years at home and abroad, attempts have been made to reduce the water holding capacity of spirulina algae mud by using dehydration processes such as continuous centrifugation, decompression suction filtration, and adsorption exchange. Due to low force performance and other reasons, none of them worked

At the same time, "random isolation of algae filaments→cultivation into algae filament groups→indoor transfer culture→outdoor expansion cultivation→drainage performance test→use for production and cultivation" is still used at home and abroad. The traditional method is cumbersome, time-consuming, and has a low success rate, and has little effect in actual production applications.

Images