Mitochondria DNA observation through MTG-DAPI double-staining of semi-thin sections of cucumber pollen

A technology of semi-thin slices and cucumber pollen, applied in the biological field, can solve problems such as no cytological evidence, and achieve a strong scientific effect

Active Publication Date: 2014-06-11
NANJING AGRICULTURAL UNIVERSITY
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Benefits of technology

In this patented technology, we discovered how certain parts of plants called chloroplasts (a type of photosynthetic pigment found inside red algae), known commonly through their role in making sugar from glucose, help us better identify what types of things they've been exposed to more accurately than before. By studying these areas over many generations, scientists were able to discover new ways to improve crops such as citrus fruits.

Problems solved by technology

This patented technology describes how certain chemical substances called guanine have special properties that allow them to attach themselves to other molecules like proteins inside plants' bodies during photosynthesis process. These attached compounds help scientists detect changes caused by these processes without being affected by any external factors involved with their production.

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  • Mitochondria DNA observation through MTG-DAPI double-staining of semi-thin sections of cucumber pollen
  • Mitochondria DNA observation through MTG-DAPI double-staining of semi-thin sections of cucumber pollen
  • Mitochondria DNA observation through MTG-DAPI double-staining of semi-thin sections of cucumber pollen

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Embodiment Construction

[0043] As a mitochondrial membrane-specific fluorescent dye, MTG is applied to the staining of mitochondria in living cells and fixed cell suspensions. The present invention applies MTG to the fluorescent staining of mitochondria in resin semi-thin sections for the first time, which expands the application of MTG and can be used for other materials. Provide basis for relevant research. As we all know, most of the inheritance of plant organelles is maternal inheritance, that is, mitochondria follow the mother cell into the fertilized egg. The related research of cucumber shows that its mitochondrial genes are inherited from the paternal line, that is, the mitochondria follow the sperm into the fertilized egg, but it has not been verified by cytological observation. The present invention conducts double fluorescent staining observation on the mitochondria in the pollen sperm cells of the cucumber adherence period, thereby confirming that the mitochondrial genome is scattered arou...

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Abstract

The invention discloses a method for performing MTG (Mito Tracker Green) and DAPI bifluorescence staining observation on resin semi-thin sections of cucumber pollen and relates to plant tissue fixation, plant anther Technovit7100 resin semi-thin sections and MTG-DAPI bifluorescence staining, belonging to the technical field of biology. The mitochondria and contained DNA in pollen reproductive cells in the cucumber adherence period are observed at the first time, the method can be used for exploring the change rule of mitochondria number in the cucumber pollen development process, and a foundation is laid for further researching related mechanisms of cucumber mitochondria paternal descent. The method also can be applied to mitochondria observation in other plant materials and is used for determining whether the plant materials have mitochondria genome paternal descent or biparental inheritance potentials.

Description

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Application Information

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Owner NANJING AGRICULTURAL UNIVERSITY
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