Device and method for classifying microalgae in ship ballast water

Abstract

The invention discloses a device and a method for classifying microalgae in ship ballast water. The device comprises a micro-fluidic chip carrying platform, a micro-fluidic chip, direct current driving assemblies, an excitation light source, light filtering assemblies, light detection assemblies and data processing assemblies, wherein the micro-fluidic chip comprises a glass slide, a liquid storage hole A, a liquid storage hole B, a liquid storage hole C, a liquid storage hole D, a liquid storage hole E, a main channel, a first focusing channel, a second focusing channel, a detection channel, an impedance pulse sensing upstream detection channel and an impedance pulse sensing downstream detection channel. By using the micro-fluidic chip as a ship ballast water microalgae classification micro-platform, the device is convenient to carry and high in detection speed. Relevant photoelectric detection equipment can be in a small-size structural form and can be conveniently transported to the field. Therefore, compared with existing large classification equipment, the device has the advantages of small size, light weight, convenience in carrying and the like, and can be handheld for quick field classification.

Description

technical field [0001] The invention relates to a technique for classifying microalgae living in ship's ballast water, in particular to a classification device and method for microalgae in ship's ballast water. Background technique [0002] The ship's ballast water is used to provide stability for the ship and adjust the draft of the ship to meet the requirements of good maneuverability of the ship. Ship ballast water contains a large number of aquatic organisms, and the invasion of alien marine organisms caused by ship ballast water has been recognized by the Global Environment Fund (GEF) as one of the four major threats to the ocean. In order to effectively deal with the problem of biological invasion caused by ballast water, the 2007 "Notice of the State Council on Printing and Distributing the "Eleventh Five-Year" Plan for National Environmental Protection" (Guofa [2007] No. A general survey of the biodiversity of key sea areas, a clear understanding of the status of in...

AI Technical Summary

Problems solved by technology

Due to the huge consumption of financial resources, material resources and manpower, this method is not realistic at present, and it is only applied to some species that are important in scientific research (such as the Human Genome Project, Rice Genome Project, etc.)

[0007] To sum up, the existing microalgae classification methods in ship ballast water have problems such as inability to quickly classify on-site, expensive equipment, huge workload, time-consuming and laborious, restricted by many external conditions, and unstable identification indicators. Algae classification and identification method is a key problem that needs to be solved urgently in the field of ship ballast water microalgae classification

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