The invention relates to a production process for separating and purifying
fiber linking proteins from pig blood. The production process comprises the following steps: (1) separating
blood plasma and blood cells from
whole blood; (2)
salting out: charging an acidic Na2HPO4-NaH2PO4
buffer solution into an upper layer of
plasma, uniformly mixing, charging
ammonium sulfate while stirring the solution so that the saturation degree reaches 20 percent, adjusting pH to be alkaline, placing the
mixed solution into a refrigerator, refrigerating the
mixed solution for 1.5 to 2.5 hours, sufficiently precipitating, finally centrifuging the solution, and obtaining a main
precipitation component, i.e.,
fiber linking proteins; (3) dialyzing and desalting: dissolving precipitates in the step (2) into an acidic PBS
buffer solution, putting the
mixed solution into a
dialysis bag, carrying out the
dialysis and concentration, thereby obtaining a crude product of the
fiber linking proteins; and (4) purifying: carrying out the
affinity chromatography for an upper sample of the fiber linking
protein crude product obtained in the step (3) by virtue of a
gelatin sepharose-4B column, and eluting by virtue of a
specific volume to obtain the purified fiber linking proteins. The production process is simple, low in requirement on needed equipment and suitable for industrialized production.