141 results about "Collagen molecule" patented technology

The invention relates to a cationic flocculant based on collagen modification and a preparation method thereof. The cationic flocculant is prepared through the following steps of: 1) taking trypsin as hydrolytic agent to hydrolyze industrial gelatine to obtain collagen solution; 2) using glyoxylic acid or gallic acid to modify the obtained collagen solution aiming at increasing the content of carboxyl groups or phenolic hydroxyl groups on collagen molecules to obtain modified collagen solution; and 3) conducting complexation to the obtained modified collagen solution and ferric sulfate or aluminum sulfate according to mass ratio being 1:2 to 1:4 to obtain the cationic flocculant based on the collagen modification. The cationic flocculant can be used for treating waste drilling fluid and solves the problems that the preparation cost of the existing flocculant is high, the synthesized or degraded products are highly toxic and carcinogenic and serious environmental pollution is caused during the current treatment of the waste drilling liquid.

Provided is a collagen structure characterized by: comprising collagen fibers of 1 to 5 µm in average diameter; and has a water content of 0 to 15 (w / w)% and a collagen density of 50 to 800 mg / cm 3 . After generating collagen fibers by neutralizing an acidic collagen solution, the resulting solution is subjected to filtration or the like to form crude collagen fibers having a collagen concentration of 12 to 50 (w / v)%. The thus obtained crude collagen fibers are molded into a prescribed shape and then dried, thereby the collagen structure can be produced. Since the collagen structure is produced using, as raw material, collagen fibers that are formed by association of collagen molecules, the collagen structure has excellent cell infiltration property. Further, since the collagen density of the collagen structure is equivalent to that of in vivo collagen tissue, the collagen structure exhibits excellent tissue regeneration capacity when filled into a defective part in vivo. Therefore, the collagen structure can be preferably used as an artificial material for regenerative medicine and the like.

This invention relates to a collagen polypeptide comprising a tripeptide motif having the formula (ProYaaGly)n, where Yaa is an O-methylated amino acid residue and “n” is the number of motif repeats. Preferred O-methylated amino acid residues at the Yaa position include (2S,4R)-4-methoxyproline. Other suitable amino acid residues at that position include O-mono or O-di-halogenated methylproline. Also, disclosed is a method of making a synthetic or a semi-synthetic collagen polypeptide molecule having increased stability relative to natural collagen. The strengthened collagen molecules are suitable for use in biomaterials for the medical field or in leather-related products prepared by the tanning industry.

The invention discloses a preparation method of a tannin-loaded collagen / cellulose composite biological adsorption material. The method comprises the following steps of dissolving cellulose powder in ion liquid which is adopted as a medium, adding sponge-shaped collagen fibers, dissolving the sponge-shaped collagen fibers, and uniformly mixing the sponge-shaped collage and the cellulose; regenerating and washing the ion liquid containing the cellulose / collagen to obtain a composite material; placing the composite material into an aqueous solution, adding plant tannin, stirring to facilitate the reaction, filtering and washing the unreacted tannin, and adding a cross-linking agent to have reaction; filtering and washing the unreacted cross-linking agent, and freeze drying the reaction product to obtain the tannin-loaded collagen / cellulose composite biological adsorption material. The cellulose is used as a substrate for fixinng little collagen, and the plant tannin is further loaded on the collagen molecules, so that the capacity of the material for adsorbing metal ions can be achieved. Metal ions can be high-efficiently and selectively adsorbed, the stability of the material is also enhanced, and the obtained composite material can be used for treating industrial waste water containing heavy metal ions such as lead and copper.

The invention relates to an extracellular matrix freeze-drying protection liquid. The extracellular matrix freeze-drying protection liquid takes normal saline as a solvent and comprises the following components: chondroitin sulfate, vitamin C, polyethylene glycol, trehalose, glucan, serine and threonine. The invention has the following advantages: the protection liquid has high penetrability, can be vitrified under a low-temperature condition, is rich in negative polar groups and hydroxyl groups, and can be efficiently bonded with collagen molecules; the stable and uniform extracellular matrix freeze-drying effect can be ensured; the protection liquid can be formed into the vitreous state at low temperature, thereby preventing ice crystals from damaging the protein structure; and a protection film is formed at the outer layer of the matrix in stead of hydrogen bonds formed by water molecules and collagen, thereby protecting the collagen function and the stability of the three-dimensional structure. The extracellular matrix freeze-drying protection liquid has low cytotoxicity, and can be used immediately after being rinsed with normal saline or sterilized water; the freeze-dried extracellular matrix can be preserved at normal temperature for more than three years when being packaged in vacuum; and the extracellular matrix freeze-drying protection liquid is suitable for the preservation of purified extracellular matrix such as collagen, elastin, glucoprotein and the like, and is also suitable for various biologic materials constructed by extracellular matrix materials.

A biofabricated material containing a network of crosslinked collagen fibrils is disclosed. This material is composed of collagen which is also a major component of natural leather and is produced by a process of fibrillation of collagen molecules into fibrils, crosslinking the fibrils and lubricating the crosslinked fibrils. Unlike natural leathers, this biofabricated material exhibits non-anisotropic (not directionally dependent) physical properties, for example, a sheet of biofabricated material can have substantially the same elasticity or tensile strength when stretched or stressed in different directions. Unlike natural leather, it has a uniform texture that facilitates uniform uptake of dyes and coatings. Aesthetically, it produces a uniform and consistent grain for ease of manufacturability. It can have substantially identical grain, texture and other aesthetic properties on both sides distinct from natural leather where the grain increases from one side (e.g., distal surface) to the other (proximal inner layers).

A biofabricated material containing a network of crosslinked collagen fibrils is disclosed. This material is composed of collagen which is also a major component of natural leather and is produced by a process of fibrillation of collagen molecules into fibrils, crosslinking the fibrils and lubricating the crosslinked fibrils. Unlike natural leathers, this biofabricated material exhibits non-anisotropic (not directionally dependent) physical properties, for example, a sheet of biofabricated material can have substantially the same elasticity or tensile strength when stretched or stressed in different directions. Unlike natural leather, it has a uniform texture that facilitates uniform uptake of dyes and coatings. Aesthetically, it produces a uniform and consistent grain for ease of manufacturability. It can have substantially identical grain, texture and other aesthetic properties on both sides distinct from natural leather where the grain increases from one side (e.g., distal surface) to the other (proximal inner layers).

The collagen base nano coloring pigment with low infrared emissitivity is in core-shell structure with inner core of nano metal oxide coloring pigment and outer collagen grafting copolymer. Collagen molecule is first adsorbed onto the surface of nano pigment particle to modify and stabilize the nano particle, lipophilic grafting branch chain is then introduced to the collagen molecule via solution grafting copolymerization under the action of initiator and the collagen grafting copolymer coats the coloring pigment. The collagen base nano coloring pigment has relatively low infrared emissivity and good dispersivity in organic solvent or water, and it has pigment particle size smaller than 100 nm and outer shell thickness less than 20 nm. It is used in preparing stealth coating and stealth costume and can realize visible light stealth and infrared stealth without damaging radar stealth.

The invention relates to a method for crosslinking collagen through an oxidized oligosaccharide. The method comprises the steps that the oxidized oligosaccharide with a certain quantity of aldehyde groups serves as a crosslinking agent, a crosslinking reaction occurs between the oxidized oligosaccharide and collagen molecules or an aggregate of the collagen molecules, then cleaning or dialyzing treatment is performed, and finally freeze drying is performed with a freeze drying machine to obtain crosslinked collagen molecules containing the oligosaccharide structure or an aggregate of the crosslinked collagen molecules. According to the crosslinked collagen obtained through the method, not only are the original performances of collagen kept, but also the oligosaccharide structure with the activity is introduced, the formed crosslinked structure can resist a certain degradation function of biological enzymes, all the basic performances are excellent, the biocompatibility is good, and the certain functionality is simultaneously achieved. The method can be applied to biological material and leather industries.

The present invention provides a compression-resistant collagen-based artificial bone repair material that could be used for bone defect repair at human load-bearing sites. Such material has a composition and structure of self-assembled nano-sized calcium phosphate salt and collagen molecules, thereby possessing a biomimetic mineralization structure similar to the natural bone. On the aspect of mechanical properties, such material has a similar mechanical strength to human cortical bone, which makes it suitable for repairing bone defects at human load-bearing sites. The present invention further provides preparation methods of such Compression-resistant collagen-based artificial bone repair material.