Preparation method of collagen sponge with recombined fiber structure
A collagen sponge and fiber structure technology, applied in the directions of pharmaceutical formulation, application, medical science, etc., can solve the problems of poor blood effect on collagen sponge, time-consuming dialysis technology, poor hemostatic effect, etc., and achieve the speed of promoting blood coagulation. , Good appearance, the effect of improving mechanical strength
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specific Embodiment 1
[0067] In this example, the recombined fibrous structure collagen sponge is obtained through the following steps 1-6:
[0068] step 1:
[0069] (1) Re-dissolve the salted-out collagen fibers with acetic acid, and the concentration after re-dissolution is 0.5 mg / mL;
[0070] (2) Using tangential flow filtration technology for collagen:
[0071] a) Purification, the purification solution is 0.01M acetic acid solution, and the molecular weight cut-off of the modified polyethersulfone hollow fiber filter tube is 50KD;
[0072] b) Concentration, this process is carried out at a temperature of 4-13°C, and a modified polyethersulfone hollow fiber filter tube with a pore size of 0.1µm is used for the final collagen concentration;
[0073] Step 2:
[0074] (1) Mix the purified and concentrated collagen solution with a certain volume of biological buffer:
[0075] The concentration of the collagen solution is 1mg / mL, the pH is 2.0; the buffer is 10×PBS, which contains 5ppm glutarald...
specific Embodiment 2
[0081] In this example, the recombined fibrous structure collagen sponge is obtained through the following steps 1-6:
[0082] step 1:
[0083] (1) Redissolve the salted-out collagen fibers with acetic acid, and the concentration after redissolution is 3.5mg / mL;
[0084] (2) Using tangential flow filtration technology for collagen:
[0085] a) Purification, the purification solution is 0.2M acetic acid solution, and the molecular weight cut-off of the modified polyethersulfone hollow fiber filter tube is 70KD;
[0086] b) Concentration, this process is carried out at a temperature of 4-13°C, and a modified polyethersulfone hollow fiber filter tube with a pore size of 0.65µm is used for final collagen concentration;
[0087] Step 2:
[0088] (1) Mix the purified and concentrated collagen solution with a certain volume of biological buffer:
[0089] The concentration of the collagen solution is 3mg / mL, the pH is 3.0; the buffer is 2×TES, which contains 10ppm carbodiimide cro...
specific Embodiment 3
[0095]In this example, the recombined fibrous structure collagen sponge is obtained through the following steps 1-6:
[0096] step 1:
[0097] (1) Re-dissolve the salted-out collagen fibers with acetic acid, and the concentration after re-dissolution is 8 mg / mL;
[0098] (2) Using tangential flow filtration technology for collagen:
[0099] a) Purification, the purification solution is 0.5M acetic acid solution, and the molecular weight cut-off of the modified polyethersulfone hollow fiber filter tube is 100KD;
[0100] b) Concentration, this process is carried out at a temperature of 4-13°C, and a modified polyethersulfone hollow fiber filter tube with a pore size of 1 µm is used for the final collagen concentration;
[0101] Step 2:
[0102] (1) Mix the purified and concentrated collagen solution with a certain volume of biological buffer:
[0103] The concentration of the collagen solution is 5mg / mL, the pH is 3.5; the buffer is 5×HEPES buffered saline solution, which c...
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