Preparation method of collagen sponge with recombined fiber structure

A collagen sponge and fiber structure technology, applied in the directions of pharmaceutical formulation, application, medical science, etc., can solve the problems of poor blood effect on collagen sponge, time-consuming dialysis technology, poor hemostatic effect, etc., and achieve the speed of promoting blood coagulation. , Good appearance, the effect of improving mechanical strength

Inactive Publication Date: 2017-11-07
SHENZHEN LANDO BIOMATERIALS
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The technical problem solved by the present invention is to provide a collagen sponge, which overcomes the problem that the traditional collagen sponge can only use the sponge structure to absorb the water in the blood for physical hemostasis, and the hemostatic effect is not good enough, so as to realize microfiber microstructure. The function of biological hemostasis
[0004] Another technical problem solved by the present invention is to provide a preparation method of collagen sponge, which overcomes the disadvantages of the existing preparation method, such as long time-consuming dialysis technology, low efficiency, and poor bleeding effect on the prepared collagen sponge, so as to efficiently prepare the collagen sponge with Collagen sponge with microfibrous structure, can stop bleeding quickly

Method used

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  • Preparation method of collagen sponge with recombined fiber structure
  • Preparation method of collagen sponge with recombined fiber structure
  • Preparation method of collagen sponge with recombined fiber structure

Examples

Experimental program
Comparison scheme
Effect test

specific Embodiment 1

[0067] In this example, the recombined fibrous structure collagen sponge is obtained through the following steps 1-6:

[0068] step 1:

[0069] (1) Re-dissolve the salted-out collagen fibers with acetic acid, and the concentration after re-dissolution is 0.5 mg / mL;

[0070] (2) Using tangential flow filtration technology for collagen:

[0071] a) Purification, the purification solution is 0.01M acetic acid solution, and the molecular weight cut-off of the modified polyethersulfone hollow fiber filter tube is 50KD;

[0072] b) Concentration, this process is carried out at a temperature of 4-13°C, and a modified polyethersulfone hollow fiber filter tube with a pore size of 0.1µm is used for the final collagen concentration;

[0073] Step 2:

[0074] (1) Mix the purified and concentrated collagen solution with a certain volume of biological buffer:

[0075] The concentration of the collagen solution is 1mg / mL, the pH is 2.0; the buffer is 10×PBS, which contains 5ppm glutarald...

specific Embodiment 2

[0081] In this example, the recombined fibrous structure collagen sponge is obtained through the following steps 1-6:

[0082] step 1:

[0083] (1) Redissolve the salted-out collagen fibers with acetic acid, and the concentration after redissolution is 3.5mg / mL;

[0084] (2) Using tangential flow filtration technology for collagen:

[0085] a) Purification, the purification solution is 0.2M acetic acid solution, and the molecular weight cut-off of the modified polyethersulfone hollow fiber filter tube is 70KD;

[0086] b) Concentration, this process is carried out at a temperature of 4-13°C, and a modified polyethersulfone hollow fiber filter tube with a pore size of 0.65µm is used for final collagen concentration;

[0087] Step 2:

[0088] (1) Mix the purified and concentrated collagen solution with a certain volume of biological buffer:

[0089] The concentration of the collagen solution is 3mg / mL, the pH is 3.0; the buffer is 2×TES, which contains 10ppm carbodiimide cro...

specific Embodiment 3

[0095]In this example, the recombined fibrous structure collagen sponge is obtained through the following steps 1-6:

[0096] step 1:

[0097] (1) Re-dissolve the salted-out collagen fibers with acetic acid, and the concentration after re-dissolution is 8 mg / mL;

[0098] (2) Using tangential flow filtration technology for collagen:

[0099] a) Purification, the purification solution is 0.5M acetic acid solution, and the molecular weight cut-off of the modified polyethersulfone hollow fiber filter tube is 100KD;

[0100] b) Concentration, this process is carried out at a temperature of 4-13°C, and a modified polyethersulfone hollow fiber filter tube with a pore size of 1 µm is used for the final collagen concentration;

[0101] Step 2:

[0102] (1) Mix the purified and concentrated collagen solution with a certain volume of biological buffer:

[0103] The concentration of the collagen solution is 5mg / mL, the pH is 3.5; the buffer is 5×HEPES buffered saline solution, which c...

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Abstract

The invention relates to a collagen sponge with a recombined fiber structure and a preparation method of the collagen sponge. The collagen sponge has a recombined fiber structure, the whole structure is in a sponge shape, and the surface of the collagen sponge has a collagen microfiber structure. Collagen molecules exist in a fiber manner and have a three-dimensional helical structure. The preparation method mainly comprises: purifying and concentrating collagen through tangential flow filtering technology, performing in-vitro molecular self-assembly, performing centrifugation, gelating, and repeated pre-freezing, unfreezing and washing, and performing freeze drying. The preparation method is high in production power and stable in process. The prepared collagen sponge with a recombined fiber structure can be widely applied to hemostasis and restoration of trauma and surgical wounds, and has bright application prospects.

Description

technical field [0001] The invention belongs to the technical field of biomedical materials, and in particular relates to a preparation method of a collagen sponge with a recombined fiber structure. Background technique [0002] Type I collagen is the most abundant protein in mammals and is found in tissues such as tendons and ligaments, skin, blood vessels, cornea, and bone. Collagen realizes the different requirements for mechanical properties in different tissues through its unique structure, and plays the role of stability, support and strength. Type I collagen fibers are soluble in acid solutions, and when the pH and temperature are elevated to a certain extent, they will reorganize to form fibers in vitro. Collagen is a special biodegradable material. Collagenase in the human body can degrade collagen into various fragments. At body temperature, these large fragments will spontaneously denature quickly and be further degraded into oligopeptides or amino acids by other...

Claims

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Application Information

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IPC IPC(8): A61L24/10A61L24/00
CPCA61L24/0036A61L24/0042A61L24/102A61L2400/04
Inventor 魏欣苗陈雪霓李丽花谭荣伟佘振定
Owner SHENZHEN LANDO BIOMATERIALS
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