Method for extracting micromolecular collagen oligopeptide from fish

A collagen oligopeptide and small molecule technology, applied in fermentation and other directions, can solve the problems of high molecular weight of collagen, incomplete degreasing, unfavorable absorption, etc., and achieve the effect of small molecular weight of collagen, shortening enzymatic hydrolysis time, and improving degreasing effect.

Inactive Publication Date: 2010-11-24
ZHANJIANG ZHONGNANDAO BIOCHEM
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Currently, enzymatic hydrolysis is used to extract small molecule collagen oligopeptides from fish scales. Some processing companies do not perform degreasing treatment before enzymatic hydrolysis, so the enzymatic hydrolysis takes a long time, which affects production efficiency; For the above-mentioned deficiencies, some enterprises have adopted the scheme of degreasing before enzymatic hydrolysis, but due to the use of saline or alcohol solution for degreasing treatment, the degreasing of this scheme is not complete, and the expected effect has not been achieved.
It is also common for the above-mentioned companies to produce collagen with a large molecular weight, which is not conducive to the absorption of the human body.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] The method for extracting small molecule collagen oligopeptides from fish comprises the following steps:

[0022] 1. Lipase degreasing:

[0023] Put the washed and dried fish scales or fish skins into the extraction tank, add water to stir evenly according to 3 times its weight, and add 0.01% lipase for enzymatic hydrolysis;

[0024] 2. Cleaning:

[0025] After letting go of the enzymatic solution in the extraction tank, add water to the extraction tank to rinse the fish scales or skin 3 times;

[0026] 3. Adjust the PH value:

[0027] Add water into the extraction tank, the amount of water added is 3 times the weight of fish scales or fish skin, heat up to 42°C, and adjust the pH value of the liquid in the extraction tank to 6.8 with 3% NaOH and HCL;

[0028] 4. Add protease for enzymatic hydrolysis:

[0029] Then lower the temperature to 38°C, add protease to carry out enzymolysis according to 1% of the weight of fish scale or fish skin, and the enzymolysis time i...

Embodiment 2

[0035] The method for extracting small molecule collagen oligopeptides from fish comprises the following steps:

[0036] 1. Lipase degreasing:

[0037] Put the washed and dried fish scales or fish skins into the extraction tank, add water to stir evenly according to 9 times of its weight, add lipase according to 0.05% of the weight of fish scales or fish skins, and carry out enzymatic hydrolysis;

[0038] 2. Cleaning:

[0039] After letting go of the enzymatic solution in the extraction tank, add water to the extraction tank to rinse the fish scales or skin 4 times;

[0040] 3. Adjust the PH value:

[0041] Add water to the extraction tank, the amount of water added is 3-15 times the weight of fish scales or fish skin, raise the temperature to 42°C-68°C, and adjust the pH value of the liquid in the extraction tank to 8 with 10% NaOH and HCL;

[0042] 4. Add protease for enzymatic hydrolysis:

[0043] Then lower the temperature to 53°C, add protease to carry out enzymolysis...

Embodiment 3

[0049] The method for extracting small molecule collagen oligopeptides from fish comprises the following steps:

[0050] 2. Lipase degreasing:

[0051] Put the washed and dried fish scales or fish skins into the extraction tank, add water to stir evenly according to 15 times of its weight, add lipase according to 1% of the weight of fish scales or fish skins, and carry out enzymatic hydrolysis;

[0052] 2. Cleaning:

[0053] After letting go of the enzymatic solution in the extraction tank, add water to the extraction tank to rinse the fish scales or skin 5 times;

[0054] 3. Adjust the PH value:

[0055] Add water to the extraction tank, the amount of water added is 15 times the weight of fish scales or fish skin, the temperature is raised to 68°C, and the pH value of the liquid in the extraction tank is adjusted to 9.9 with NaOH and HCL with a concentration of 17%.

[0056] 4. Add protease for enzymatic hydrolysis:

[0057] Then lower the temperature to 68°C, add proteas...

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PUM

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Abstract

The invention discloses a method for extracting micromolecular collagen oligopeptide from fish, comprising the following step of: lipase degreasing: putting cleaned and dried scales or fishskin into an extraction pot; evenly mixing by adding water according to weight of 3-15 times; carrying out zymohydrolysis by adding lipase according to 0.01-1 percent of the weight of the scales or the fishskin; and then, cleaning, regulating a pH value, carrying out zymohydrolysis by adding the prolease, blanching, filtering, concentrating and drying to obtain the micromolecular collagen oligopeptide. The prolease comprises the following zymohydrolysis step of: carrying out zymohydrolysis by adding the prolease according to 1-4 percent of the weight of the scales or the fishskin for 1-6 hours at the temperature of 38-68 DEG C. Because the lipase is adopted to degrease the scales or fishskin, the method increases degreasing effect, thereby greatly reducing zymohydrolysis time of the prolease and improving production efficiency; and moreover, the processed collagen has lower molecular weight and is beneficial to being absorbed by a human body.

Description

technical field [0001] The invention relates to a method for extracting collagen oligopeptides, in particular to a method for extracting small molecule collagen oligopeptides from fish by means of enzymatic hydrolysis. Background technique [0002] Collagen protein is an important functional protein, which is closely related to cell proliferation, differentiation, exercise, immunity, joint lubrication, wound healing and so on. Due to the special functions of collagen, its extracts have been widely used in medicine, food, daily chemical industry, biosynthesis and other industrial fields, such as medical capsules, surgical materials, edible gelatin, photographic gelatin, cosmetics, etc. The raw materials of collagen products currently produced mainly come from the skin, bone and tendons of terrestrial mammals such as pigs and cattle. Due to the deterioration of the global ecological environment in recent years, many epidemics such as mad cow disease and foot-and-mouth disease ...

Claims

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Application Information

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IPC IPC(8): C12P21/06
Inventor 林国张嘉强马晓英
Owner ZHANJIANG ZHONGNANDAO BIOCHEM
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