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Method for extracting collagen from crucian carp skin

A collagen and fish skin technology, applied in chemical instruments and methods, animal/human proteins, connective tissue peptides, etc., can solve the problems of low collagen extraction rate, unsuitable for factory production, low collagen activity, etc. The effect of high extraction rate, less equipment and high biological activity

Inactive Publication Date: 2017-10-10
兰溪市沉默生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The extraction rate of the above-mentioned collagen is low, the activity of the obtained collagen is low, the product quality is relatively poor, and it is not suitable for factory production

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] The method for extracting collagen from crucian carp skin includes pretreatment, washing, enzymatic extraction, salting out, dialysis and freeze-drying. The above method has a high extraction rate of collagen, which can reach more than 97.8%. The preparation steps are simple, and the amount of equipment required is small. Each step has accurate data, good reproducibility, and is suitable for large-scale production; The raw material is prepared from fish skin, the cost is low, the resource utilization rate of crucian carp is improved, and the added value of crucian carp is increased; the prepared collagen has high biological activity and can be used in the fields of medicine, food, health care products and cosmetics. The enzymatic extraction step is to add pepsin, active polypeptide and acetic acid solution to the fish skin, enzymatically hydrolyze, and centrifuge to obtain the supernatant. The triple helix structure of collagen is quite stable to pepsin, pepsin can sele...

Embodiment 2

[0026] The method for extracting collagen from crucian carp skin, comprises the following steps:

[0027] 1) Pretreatment: soak crucian carp skin in 0.04mol / L sodium hydroxide solution containing 1% hydrogen peroxide for 24 hours, and the solution temperature is 10°C;

[0028] 2) Washing: Stir the pretreated solid-liquid mixture with a food mixer at a high speed of 6000r / min for 5 minutes, filter the precipitate with gauze, first wash the precipitate with 10% n-butanol, then rinse with tap water, and finally rinse with distilled water until neutral ;

[0029] 3) Enzymatic extraction: add pepsin, active polypeptide and acetic acid solution to the fish skin, enzymatically hydrolyze, centrifuge to get the supernatant. The volume ratio of fish skin to acetic acid solution is 1:50, the pepsin:fish skin dry weight ratio is 1:10, the active polypeptide is 4% of the weight of pepsin, and the concentration of acetic acid solution is 0.5mol / L. The amino acid sequence of the active pol...

Embodiment 3

[0034] The method for extracting collagen from crucian carp skin, comprises the following steps:

[0035] 1) Pretreatment: soak crucian carp skin in 0.05mol / L sodium hydroxide solution containing 1% hydrogen peroxide for 20 hours, and the solution temperature is 4°C;

[0036] 2) Washing: Stir the pretreated solid-liquid mixture with a food mixer at a high speed of 8000r / min for 4 minutes, filter the precipitate with gauze, first wash the precipitate with 10% n-butanol, then rinse with tap water, and finally rinse with distilled water until neutral ;

[0037] 3) Enzymatic extraction: add pepsin, active polypeptide and acetic acid solution to the fish skin, enzymatically hydrolyze, centrifuge to get the supernatant. The volume ratio of fish skin to acetic acid solution is 1:45, the pepsin:fish skin dry weight ratio is 1:8, the active polypeptide is 3.5% of the weight of pepsin, and the concentration of acetic acid solution is 0.5mol / L. The amino acid sequence of the active pol...

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Abstract

The invention discloses a method for extracting collagen from crucian carp skin. The method comprises steps of preprocessing, washing, enzymatic extraction, salting out, dialysis and freeze drying. The method has the following beneficial effects: the method adopts simple preparation steps andrequires a small quantity of devices, accurate data is provided for each step of operation, the reproducibility is good, and the method is suitable for large-scale production; the collagen is prepared from the crucian carp skin, namely, the crucian carp processing leftover as a raw material, the cost is low, the rate of utilization of the crucian carp is increased, and added value of the crucian carp is increased; the prepared collagen is high in biological activity and can be applied to the fields of drugs, food, health care products, cosmetics and the like; the speed for extracting the collagen with an enzymatic hydrolysis method is high, non-helical telopeptide can be selectively removed by pepsin, so that collagen molecules can be dissolved out, the triple helix structure of the collagen is retained, and the biological activity of the collagen is maintained.

Description

technical field [0001] The invention relates to the technical field of collagen preparation, in particular to a method for extracting collagen from crucian carp skin. Background technique [0002] Collagen, also known as collagen, is a fibrous protein formed by intertwining three peptide chains. It is a glycoprotein that contains a large amount of glycine, proline, hydroxyproline and sugar. It is widely found in animal skin, bones, In tissues such as cartilage and tendon, it is the most abundant type of protein in the body. There are 19 types of collagens that have been discovered, among which type I collagen, type II collagen and type III collagen are the main components of collagen fibers, which mainly exist in the connective tissue of animals, and play the functions of supporting organs and protecting the body. structural proteins. [0003] Animal skin collagen is mainly type I collagen, which is usually a colorless, transparent, unbranched fibril with a molecular weigh...

Claims

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Application Information

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IPC IPC(8): C12P21/06C07K14/78C07K1/36C07K1/34C07K1/30
CPCC12P21/06C07K14/78
Inventor 周建波
Owner 兰溪市沉默生物科技有限公司
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