The invention relates to the technical field of biological tissue engineering, and discloses an organ model construction method based on an organ chip. The organ model construction method comprises the following steps of coating the organ chip; then inoculating cells into first culture micropores and / or second culture micropores; and correspondingly, adding a culture solution into a liquid storage hole and / or a fluid operation hole, and carrying out single-hole culture, co-culture or sequential culture on the cells in the first culture micropores and / or the second culture micropores to obtain the corresponding organ model. According to the embodiment of the invention, on the basis of a specific organ chip, a dynamic culture mode is adopted, external complex equipment is not needed, constant current is realized, the real physiological condition in a body is simulated, and a highly bionic organ model is obtained; the construction mode is flexible, and the construction requirements of different organ models are met; and the constructed organ model has high transmembrane resistance, more tight junction proteins are expressed, and the barrier function is stronger. The invention further discloses the organ model.