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Organ model construction method based on organ chip, and organ model

An organ chip and construction method technology, applied in biochemical equipment and methods, biochemical instruments, tissue cell/virus culture devices, etc., can solve the problems of single construction method, single organ model evaluation, and low bionicity of organ models. Achieve the effects of flexible construction methods, more expression of tight junction proteins, and high transmembrane resistance

Active Publication Date: 2021-04-20
BEIJING DAXIANG BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The embodiments of the present disclosure provide a method for constructing an organ model based on an organ chip, an organ model and its application, so as to solve the problem that the organ model constructed by the existing construction method is low in bionicity, has a single construction method, and can only evaluate a single organ model

Method used

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  • Organ model construction method based on organ chip, and organ model
  • Organ model construction method based on organ chip, and organ model
  • Organ model construction method based on organ chip, and organ model

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Experimental program
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Effect test

Embodiment 1

[0135] combine image 3 and Figure 4 As shown, an organ chip includes a chip body, and the chip body includes a plurality of culture modules, and each culture module includes a liquid storage hole 21, a first culture microwell 22, a second culture microwell 23 and two fluid operation Holes 24 (a pair of fluid operation holes 24), and through microchannels 25; from the surface of the chip body to the inside, the liquid storage holes 21, the first culture microholes 22 and the second culture microwells 23 are sequentially communicated with each other, and A thin film is arranged between the first culture microwell 22 and the second culture microwell 23; the two fluid operation holes 24 are also extended from the surface of the chip body to the inside, and are respectively located on both sides of the liquid storage hole 21; the first side The fluid operation hole 2401 communicates with the second culture microwell 23 through the first side through microchannel 251 , and the se...

Embodiment 2

[0145] A method for constructing a blood-brain barrier model based on the organ chip of embodiment 1, comprising the following steps:

[0146] S31 , coating the organ chip to obtain the coated organ chip. Specifically include:

[0147] Prepare the coating solution: dilute type I rat tail collagen with acetic acid to obtain a coating solution with a concentration of type I rat tail collagen of 0.4 mg / mL. Add 40 μL of coating solution to the fluid operation well of the organ chip (so that each fluid operation well has 20 μL of coating solution), add 20 μL of coating solution to the storage well, and incubate dynamically on a shaker for 2 hours. Buffered saline solution (PBS) was used for dynamic washing to obtain coated organ chips.

[0148] S32. Inoculating cells into the second culture microwells coated with the organ chip; specifically including:

[0149] The brain microvascular endothelial cell line hCMEC / D3 was digested to obtain a density of 1×10 7 cells / mL of brain mi...

Embodiment 3

[0159] A method for constructing a blood-brain barrier-brain metastases tumor co-cultured organ model, such as figure 2 shown, including the following steps:

[0160] S41 , coating the organ chip to obtain the coated organ chip. Specifically include:

[0161] Prepare the coating solution: dilute type I rat tail collagen with acetic acid to obtain a coating solution with a concentration of type I rat tail collagen of 0.4 mg / mL. Add 100 μL of coating solution to the fluid operation well of the organ chip (so that each fluid operation well has 50 μL of coating solution), add 50 μL of coating solution to the storage well, and incubate dynamically on a shaker for 2 hours. Buffered saline solution (PBS) was used for dynamic washing to obtain a coated organ chip.

[0162] S42. Inoculating cells into the first culture microwell of the coated organ chip, and then adding culture solution into the liquid storage hole to cultivate the cells in the first culture microwell; specifically...

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Abstract

The invention relates to the technical field of biological tissue engineering, and discloses an organ model construction method based on an organ chip. The organ model construction method comprises the following steps of coating the organ chip; then inoculating cells into first culture micropores and / or second culture micropores; and correspondingly, adding a culture solution into a liquid storage hole and / or a fluid operation hole, and carrying out single-hole culture, co-culture or sequential culture on the cells in the first culture micropores and / or the second culture micropores to obtain the corresponding organ model. According to the embodiment of the invention, on the basis of a specific organ chip, a dynamic culture mode is adopted, external complex equipment is not needed, constant current is realized, the real physiological condition in a body is simulated, and a highly bionic organ model is obtained; the construction mode is flexible, and the construction requirements of different organ models are met; and the constructed organ model has high transmembrane resistance, more tight junction proteins are expressed, and the barrier function is stronger. The invention further discloses the organ model.

Description

technical field [0001] The present application relates to the technical field of biological tissue engineering, for example, to an organ chip-based organ model construction method and an organ model. Background technique [0002] Cancer is a disease caused by the loss of normal regulation and excessive proliferation of body cells. Cancer cells infiltrate surrounding tissues and metastasize to other parts of the body via the circulatory system and / or lymphatic system. There are various types of cancer, and the severity of the disease depends on the location of the cancer cells, the degree of malignancy and whether they have metastasized. Brain metastases are common intracranial tumors secondary to extracranial tumors, and the main primary tumor types are lung cancer, breast cancer, and melanoma. The incidence of brain metastasis of malignant tumors is extremely high, and the prognosis is usually poor. For example, 20% to 30% of patients with non-small cell lung cancer will ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12M3/00C12N5/07
Inventor 肖荣荣周宇
Owner BEIJING DAXIANG BIOTECH CO LTD
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