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Effect of Panx1 protein in preparation of medicine for preventing and treating neuropathic pain

A neuropathy, protein inhibitor technology, applied in the field of biomedicine, can solve problems such as little known

Pending Publication Date: 2021-11-09
NANTONG UNIVERSITY
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, Schwann cells can secrete a variety of pain-related pro-inflammatory / anti-inflammatory factors after peripheral nerve injury, but their roles and possible mechanisms in the occurrence, development and maintenance of neuropathic pain are still poorly understood

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  • Effect of Panx1 protein in preparation of medicine for preventing and treating neuropathic pain
  • Effect of Panx1 protein in preparation of medicine for preventing and treating neuropathic pain
  • Effect of Panx1 protein in preparation of medicine for preventing and treating neuropathic pain

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Embodiment 1

[0012] The inventor's previous research found that Schwann cells cultured in vitro mainly express Panx1, which plays an important regulatory role in the process of ATP release induced by hypotonicity. Therefore, the inventors speculate that Schwann cell Panx1 may be a target molecule for the regulation of neuropathic pain.

[0013] In the present invention, the pain model of sciatic nerve chronic compression injury (CCI) mice was firstly used, and the expression changes of Pannexins after injury were detected by qPCR, and the expression changes of Panx1 after injury were further detected by Western blotting and immunofluorescence; 10 days after CCI injury, The Panx1 inhibitor carbenoxolone (CBX), probenecid, and pannexin 1 peptidomimetic were administered subepithelially 10 Panx 1, detect the changes of mechanical pain and thermal pain in mice, and detect the expression changes of Panx1 at different time points after probenecid administration. These help to understand the rol...

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Abstract

The invention discloses effect of connexin Panx1 in preparation of a medicine for preventing and treating neuropathic pain, and belongs to the technical field of biological medicines. Main expression Panx1 in Schwann cells is determined, and the expression quantity of the Panx1 can be remarkably increased due to chronic neuropathic pathological injury; an inhibitor and a mimic peptide of the Panx1 can remarkably inhibit mechanical pain and heat pain caused by chronic neuropathic pathological injury of sciatic nerves, and meanwhile, a Panx1 inhibitor probenecid can remarkably inhibit the expression of the Panx1 caused by injury. Therefore, powerful basic research evidence is provided for treating patients with chronic neuropathic pathological pain by clinically utilizing the Panx1 inhibitor in the future.

Description

technical field [0001] The invention belongs to the technical field of biomedicine, and specifically relates to the role of gap junction protein Panx1 in the preparation of medicines for preventing and treating neuropathic pain. Background technique [0002] Neuropathic pain is a chronic pain caused by primary damage and dysfunction of the nervous system. Its clinical characteristics are stubbornness, high incidence, long course, and hyperalgesia persists in weeks, months or even years. The key problem in the current treatment of chronic neuropathic pain is that its occurrence and development mechanism is still not fully understood. Many years of research have focused on neurons, and found that peripheral sensitization caused by increased excitability of primary sensory neurons and central sensitization caused by inflammatory mediators acting on central neurons are important factors for the occurrence and maintenance of neuropathic pain. Pathological basis. However, in re...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K45/00A61P25/00A61P29/00C12Q1/6883G01N33/68A61K31/56A61K31/195A61K38/17
CPCA61K45/00A61P25/00A61P29/00C12Q1/6883G01N33/6893A61K31/56A61K31/195A61K38/1709C12Q2600/158C12Q2600/136G01N2800/2842G01N2500/04
Inventor 韦中亚陈罡王茜凌卓敏
Owner NANTONG UNIVERSITY
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