93results about How to "Activity does not affect" patented technology

The invention relates to a water-soluble active extract derived from fruit peels, the preparation method and the application thereof. The fruit peel is treated by the steps of drying, pulverization, extraction, flocculation, separation and concentration, to prepare a water-soluble active extract. Then, the extract is added in cosmetic matrixes to allow a cosmetic to have skin-whitening and anti-skin ageing functions. The fruit peel extract is obtained after the screening of tyrosinase-inhibiting, antioxidant and free radical-scavenging activities in the water-soluble extracts of fruit peels such as Citrus peels, and has skin-whitening and anti-skin ageing effects. The water-soluble active extract has the advantages of easily-accessible raw materials, renewable resources and simple preparation method. The obtained extract has good stability and durable effect and serves as cosmetic additives in any stage of cosmetic production, without the risks of solvent residue and microbial contamination.

The invention relates to the pesticide remains fast detecting reagent box and its using method. The reagent comprises the extracting reagent, the base material, the color-showing agent and enzyme. It judges the whether the pesticide remains surpass the standard amount through the color-comparison reaction. The manufacturing of the box is very simple, and cost is very low and the detection process is fast.

The invention relates to corrosion inhibitor for hydrogenation device with constituent as: 10-30% fat nitrogen-containing compound, 10-20% aromatic sulfur-containing compound, 0-25% organic acid esters, 15-70% aromatics, and 0-25% imidazoline derivative. A little this product can effective inhibiting corrosion and salt dirty without any negative effect to product property and successive process fit to high-concentration H2S-HCl-H2O acidic or H2S-HCl-NH3-H2O basic system. It can form dense monomolecular layer protection membrane on metal surface to prevent hydrogen embrittlement phenomenon and fit to heat exchanger, air cooler and others.

The invention discloses a natto nutritional health food. The natto nutritional health food is prepared from the following raw materials in parts by weight: 20-40 parts of natto powder, 10-25 parts of soybean peptide powder, 10-20 parts of spirulina, 10-15 parts of soybean phospholipids, 10-15 parts of isomalt, 5-10 parts of kudzu vine root extract, 5-10 parts of chitosan oligosaccharide, 5-10 parts of soybean dietary fiber powder, 5-10 parts of arabinose, 2-5 parts of maltodextrin and 1-3 parts of microcrystalline cellulose. The natto nutritional health food is easily absorbed by a human body, and also has the effects of preventing and curing diseases and beatifying in addition to relatively high nutritive value; as a result, the waste generated in the body can be eliminated immediately and domestic and overseas polypeptide food varieties can be enriched.

The invention discloses plectasin as well as a gene and a preparation method thereof. A nucleotide sequence of the gene of plectasin is shown in SEQ ID No.1 in a sequence table. The invention discloses a gene sequence of plectasin fusion protein, an amino acid sequence, a recombinant expression vector containing the gene of plectasin and a recombinant expression transformant containing a gene coding the plectasin fusion protein, wherein the gene sequence of the plectasin fusion protein contains enzyme digestion sites of His-tag and TEV enzymes. The preparation method of the plectasin comprises the following steps of: cultivating the recombinant expression transformant; separating and purifying the obtained plectasin fusion protein; adding TEV enzyme and carrying out enzyme digestion; and separating and purifying to obtain plectasin protein. By adopting the preparation method of the plectasin, expression quantity of the plectasin is greatly increased, and the plectasin is simpler and easier to separate and purify. The purified plectasin can effectively inhibit growth of gram positive bacteria, bacillus subtilis and methicillin-resistant staphylococcus epidermidis golden yellow staphylococci.

The invention provides a sponge loaded photocatalyst, which is prepared from sponge, a Linker reagent and a photocatalyst, wherein the sponge contains surface groups; the Linker reagent at least contains two active functional groups; the photocatalyst is an organic photocatalyst or a metal complex photocatalyst; the Linker reagent is chemically bonded with the surface groups of the sponge through the active functional groups, and is then loaded on the surface of the sponge; and the photocatalyst is connected onto the surface of the sponge through being bonded with the active functional groups of the Linker reagent.

The method for preparing electrochemical biological transducer with TiO2 gel film includes the following steps: a) preparing TiO2 film, soaking ITO electrode in 2-8 mol / L of sodium hydroxide solution for 5-12 hour, immersing the electrode in 50-300 mM butyl exter titanate for 2-5 minute, immersing the electrode in anhydrous alcohol for 1-3 minute and then in secondary water for 1-3 minute; b) immersing electrode obtained from step a) in aqueous solution of zyme (concentration of 2-4 mg / L) for 30-120 minute. The prepared transducer can hold its activity for 14 days and it can be reused.

The invention discloses a polyethylene glycol modified tumor angiogenesis inhibitor HM-1 and application thereof, and belongs to the field of polypeptide drugs. The peptide sequence adopted in the polyethylene glycol modified tumor angiogenesis inhibitor HM-1 is mPEG-Arg-Gly-Ala-Asp-Arg-Ala-Gly-Gly-Gly-Gly-Arg-Gly-Asp, wherein mPEG is mPEG-SC, mPEG2-NHS, mPEG-ALD or mPEG-bALD, and the molecular weight range is 500 to 40000. The polypeptide modified by polyethylene glycol has the capacity of restraining endothelial cell migration and integrin affinity and binding, and can be applied to prevention and treatment of tumours, various phlegmonosis and neovascular eye diseases. The polyethylene glycol modified tumor angiogenesis inhibitor is prepared through an artificial synthesis method.

The invention provides an umbilical cord tissue freeze preservation and unfreezing method. According to the umbilical cord tissue freeze preservation and unfreezing method, cell aggregate freeze preservation is adopted instead of single cell freeze preservation, so that cell damage apoptosis rate is reduced, and survival rate is increased; cell adhesion molecules are added to promote bonding of cells, reduce the amount of single cells, and increase cell survival rate; ultrasonic treatment is adopted so as to ensure full penetration of a refrigeration protective solution and an unfreezing protective solution into intercellular spaces, solutions are dispersed into small liquid drops, and the permeability is improved; a cell activity inhibitor is adopted for reversible inhibition of physiological activities in cells, and improving of freeze preservation effect without influencing cell activity. The umbilical cord tissue freeze preservation and unfreezing method is capable of reducing physical and chemical damages on cells in freezing and unfreezing process, and increasing cell survival rate and unfreezing rate, so it is convenient for subsequent using.

The invention discloses a device and a method for quickly starting and maintaining short-cut nitrification of municipal sewage based on p-chloro-m-xylenol, and belongs to the field of biological sewage treatment. The device mainly comprises a municipal sewage raw water tank, a municipal sewage short-cut nitrification device, a p-chloro-m-xylenol sludge treatment device and a sludge reflux device,the method comprises the following steps: firstly, adding 60mg / L-70mg / L of the p-chloro-m-xylenol into a reactor in a first period; stirring for 15-20 hours to inhibit the activity of nitrifying bacteria, and then laterally treating part of activated sludge instead of adding an inhibitor into the reactor, so that on one hand, the activity of AOB is rapidly recovered, and on the other hand, the activity of NOB is continuously inhibited, and accumulation of nitrite and starting normal-temperature short-cut nitrification of municipal sewage can be realized. According to the method, the activity of the AOB can be recovered at the highest speed, the NOB can be elutriated step by step, and stable short-cut nitrification can be maintained.

The invention discloses novel waterborne epoxy resin anticorrosive coating. The novel waterborne epoxy resin anticorrosive coating comprises surface coating and backing coating. The surface coating comprises, by weight, 38-42 parts of waterborne epoxy resin emulsion, 15-17 parts of waterborne epoxy resin curing agents, 10-12 parts of deionized water, 8-10 parts of nanometer-sized titanium dioxide,6-8 parts of activated carbon, 2-4 parts of UV (ultraviolet) varnish, 1-2 parts of colloidal copper catalysts and 2-4 parts of No.1 antirust grease. The novel waterborne epoxy resin anticorrosive coating has the advantages that the nanometer-sized titanium dioxide is added into the surface coating, biological enzymes are added into the backing coating, accordingly, harmful substances such as formaldehyde on brushed objects can be removed, and indoor formaldehyde further can be removed; the UV varnish added in the surface coating needs to be baked by ultraviolet rays, accordingly, the nanometer-sized titanium dioxide can be conveniently photocatalyzed by the ultraviolet rays, formaldehyde removal can be accelerated, influence on the activity of the biological enzymes in the backing coatingcan be prevented, and the integral novel waterborne epoxy resin anticorrosive coating has diversified functions and is high in practicality.

The invention relates to the technical field of immunoassay, in particular to a kit for preparing a CA125 surface Tn antigen by magnetic particle chemiluminescence immunoassay and a preparation methodthereof. The kit comprises a magnetic separation reagent coupled with a Fab fragment of a CA125 antibody, a biotin-lectin reagent, and a streptavidin-biotin-alkaline phosphatase coupling reagent, wherein the biotin-lectin reagent containing sialidase is capable of identifying the Tn antigen. The magnetic separation reagent is specifically bound to the CA125 antigen; the biotin-lectin specificallyrecognizes the Tn antigen of the CA125 antigen surface; the streptavidin-biotin-alkaline phosphatase coupling reagent is specifically bound to the biotin-lectin reagent to realize amplification of the detection signal; the sialidase hydrolyzes a glucosidic bond connected with the sialic acid on the Tn antigen surface and the sialic acid is released to expose the Tn antigen. Therefore, the detection accuracy, sensitivity and linear range are improved. The kit has advantages of high specificity and high sensitivity and is capable of realizing fully automated testing.

The invention discloses a biological enzyme-catalyzed wall-breaking microalgae grease extraction method. The method comprises the following four steps: microalgae culture centrifugal screening, protease treatment and boiling, ultrasonic treatment after combined enzyme treatment and distillation extraction. Through change of the addition order of enzymes and control on the temperature of a solution, the activity of the enzymes is maximized, the use amount of the enzymes is reduced, and decomposition of whole microalgae cells is accelerated finally through short-term ultrasonic treatment; meanwhile, the decomposition products is isolated from grease on microalgae, and subsequent grease extraction is facilitated; and not only can the extraction rate of internal grease of the microalgae be improved, but also the use amount of the enzymes is reduced, the extraction cycle is shortened, and the extraction cost is reduced greatly.

The invention discloses a method for preventing W loss by capturing a W catalyst in oxidative cracking reaction. The method particularly includes the steps: uniformly mixing a solid adsorbent, the W catalyst, hydrogen peroxide and reaction substrates; performing stirring reaction on mixture; centrifugally separating solid after reaction is completed; recycling the solid by a post-processing step;mixing the solid, the hydrogen peroxide and the reaction substrates; repeating catalytic reaction. W dissolved in reaction solution is adsorbed by a SnO2 solid adsorbing agent, a carrier of the W is formed, activity of catalytic reaction cannot be reduced, the W is recycled and then can be repeatedly used for the catalytic reaction, the activity of catalytic reaction cannot be affected by the solid adsorbing agent, the solid adsorbing agent can be repeatedly used, SnO2 cannot decompose the hydrogen peroxide when the SnO2 has a high PZC value, the SnO2 added into WO3 / H2O2 and H2WO4 / H2O2 systems, tungsten-containing negative ions can be effectively dissolved, a new catalyst is combined, the W loss is prevented, and the service life of the catalyst is prolonged.

The invention discloses a method for extracting crystals of urease in canavalia ensiformis. The method comprises the following steps of 1, extraction, wherein canavalia ensiformis is ground into powder, an extracting solution is added after screening is carried out, full stirring is carried out, urease in the canavalia ensiformis powder is all digested, the leaching liquor is centrifugalized at room temperature, and the supernate is collected; 2, rough precipitating, wherein the supernate stands and refrigerated, centrifugalization is carried out, precipitate is collected, and rough urease precipitate is obtained; 3, cooling crystallization, wherein the rough urease precipitate is dissolved in a sodium citrate solution and stirred to be fully dissolved, centrifugalization is carried out, supernate is collected, a protein solution is obtained, a precipitator solution is slowly added to the protein solution, crystallization starts to be carried out after the materials are mixed to be uniform, a crystal solution is obtained, the crystal solution is subjected to centrifugal separation, urease crystal precipitate is collected and freeze-dried, and a urease product with the purity exceeding 96% is obtained. The method is easy to operate, short in technological process and low in energy consumption, a conventional solid-liquid separation means is adopted in the preparation process, and the urease gross activity recovery rate reaches 60% or above.

The invention discloses a benzofuran compound and a medical application thereof, and relates to a compound and an application thereof. A general formula of the compound is as shown in the specification, wherein R and R' are independently selected from hydrogen, methyl, ethyl or tertiary butyl; or the R and R', together with nitrogen atoms connected to the R and R', form pyrrolidyl, piperidyl or morpholinyl. The compound, disclosed by the invention, through an enzyme inhibiting property efficacy and other biochemical parameters thereof, can achieve an effective treatment effect by an expected usable dosage range which is about 0.01-10mg / kg per day; the compound is bound or embedded in a soluble and / or biodegradable polymer and is coated on a Stent's impression material; such polymer contains polyvinylpyrrolidone, polyhydroxy-propyl isobutylene amide-phenol, and a cross-linked or amphiphilic block copolymer of hydrogel.

The invention provides a recovery process for purifying sulfur with coal-to-methanol synthesis gas. The process comprises the following steps: performing replacement on a reactor and a system nitrogentemperature rise system, and heating a sulfur recovery catalyst bed by adopting nitrogen; clearing sulfur after stopping sulfur recovery, controlling the temperature of a heater at the inlet of the reactor, heating by medium pressure steam, ensuring the temperature of the inlet of the bed to be 120-260 DEG C, and clearing sulfur while controlling the bed temperature to 120-260 DEG C. The volume content of H2S and S at the outlet of the bed is less than 0.01%, and when no liquid sulfur is discharged during drain recovery of a system condenser, the operation of clearing sulfur with nitrogen isended. According to the process disclosed by the invention, catalyst starting can be completed, the activity temperature is met, and in the processes of raising the temperature of the sulfur recoverycatalyst and stopping sulfur clearing, incineration side-reactions are fundamentally avoided, so that the influences of carbon deposit and water vapor on the catalyst activity can be eliminated. In addition, the inert gas nitrogen is taken as the sulfur-clearing carrier, so that unsafe factors brought by leakage are fundamentally eliminated, and intrinsic safety is realized.

The invention relates to a capsule shell and a preparation method thereof. The capsule shell comprises 1-50wt% of L-arabinose. The capsule shell is conductive to relieving irritation of drugs to intestinal tracts and shortening the drug absorption time.

The invention discloses a bioadhesive temperature-sensitive material, a preparation method thereof and application of a drug carrier. The material does not need to be additionally provided with a cross-linking agent or subjected to other treatment, an aqueous solution of the material is dehydrated and gelled by polyoxyethylene-polyoxypropylene-polyoxyethylene chains along with temperature rise, the material has good temperature sensitivity, and meanwhile, catechol terminal groups of the material can be spontaneously and chemically cross-linked with one another or can be spontaneously and chemically cross-linked with amino on the surface of a living body to generate super-strong bioadhesive force. The bioadhesive temperature-sensitive material is combined with a temperature-sensitive gel matrix and a stabilizer and can be used as a delivery carrier of bioactive factors. Bioadhesive temperature-sensitive drug-loaded hydrogel can be directly used for perfusion and administration in a cavity, can quickly respond to body temperature change for gelling, meanwhile can be uniformly adhered to the surface of mucosa of the cavity, slowly releases the bioactive factors and promotes repair ofthe ulcerative mucosa.

The invention belongs to the field of daily chemicals, and concretely relates to an antibiosis detergent powder, and a preparation method and an application thereof. The antibiosis detergent powder includes an anionic surfactant, a nonionic surfactant, sodium carbonate, sodium silicate, carboxymethylcellulose sodium, sodium hydroxide, 4A zeolite, a whitener, biological enzyme, an essence, nano-silver and sodium sulfate. The detergent powder prepared in the invention has the advantages of low cost, good decontamination effect, and effective inhibition and elimination of common bacteria on clothes and textiles, and is especially suitable for washing clothes in hotels, hospitals and other public places.

The invention discloses a method for constructing tissue engineering artificial liver-like tissue. The method comprises the steps as follows: three-dimensional culture of a hepatocyte suspension is performed to form cell clustered-balls; the plurality of the cell clustered-balls are assembled around a central reserved hole to form a clustered-ball microarray; a plurality of the clustered-ball microarrays are closely arranged, packaged and fixed to form artificial liver-like tissue. The method for constructing tissue engineering artificial liver-like tissue is convenient to operate and favourable to large-scale production; and the obtained artificial liver-like tissue is relatively high in bionic degree and has a clinical application prospect.

The invention provides a membrane electrode and a preparation method thereof, a sensor by employing a membrane electrode, an electrochemical workstation and application thereof. The membrane electrodecomprises an electrode and a modified membrane on the surface of the electrode, the modified membrane is a mixture of chitosan and alizarin red S; the modified membrane of the chitosan and the alizarin red S is deposited at the surface of the electrode to allow the obtained membrane electrode to have high sensitivity, high sensing effect and have responsiveness to the current change; the preparation method of the membrane electrode is simple, easy to obtain raw materials, low in cost and easy to implement and can be suitable for industrial large-scale production application; and the sensor comprising the membrane electrode is high in detection sensitivity and good in accuracy; the electrochemical workstation comprising the sensor can achieve the qualitative and quantitative detection of aluminum ions, and is high in sensitivity, small in relative standard deviation and high in accuracy.

A multi-phase bimetal catalyst system for carbonylating reaction of methanol to obtain acetic acid and methyl acetate is composed of cocatalyst (iodomethane) and primary catalyst Ni-La / Ys, where Ys is the carbonified high polymer. Its preparing process includes preparing carrier of primary catalyst, carbonifying carrier, and preparing primary catalyst.

The invention provides a process for preparing an SCR reducing agent from a coking residual ammonia wastewater as a raw material. The process is characterized by comprising the following steps: condensing ammonia vapor generated by a dephlegmator of an ammonia stripping system of a gas purification workshop in a coking plant to obtain coking strong ammonia water; removing impurities from the coking strong ammonia water by a filter and deoiling by an adsorption tower; adding an NaOH alkali liquor to the ammonia water, mixing evenly, and then conveying to a distillation tower to carry out secondary distillation; processing the ammonia vapor at the tower top through the dephlegmator and then processing by the condenser, so as to obtain refined strong ammonia water; adopting coking ammonia water generated by ammonia distillation of the coking residual ammonia wastewater as a preparation raw material of the SCR reducing agent, and refining the coking strong ammonia water to reach the requirements of the reducing agent used for SCR catalytic reduction, so that the denitration cost is reduced when the denitration efficiency is ensured.

The invention discloses a method for breeding, culturing and preserving paratylenchus by using carrot callus, belonging to the field of plant-parasitic nematode culture. The method comprises the following steps of: (1) under a sterile condition, soaking carrots with alcohol, scorching, peeling and slicing the carrots, putting carrot slices into a sterilized culture dish, sealing an opening of the sterilized culture dish, putting into a culture box, and culturing till the carrot slices grow callus; (2) sterilizing the paratylenchus with a streptomycin sulfate solution, centrifuging and collecting, eluting with sterile water, centrifuging and collecting again, preparing a paratylenchus suspension with sterile water, and dripping the suspension on the carrotcallus; and (3) putting the carrot callus inoculated with the paratylenchus into the culture box, and culturing. In the method, a culture medium is not required for culturing, so that the operation is simple and convenient; and sterilization of the streptomycin sulfate solution does not affect the activity of the paratylenchus, so that the aim of breading the paratylenchus simply, efficiently and largely is achieved, the paratylenchus can be preserved for a long time, and parasitism and pathogenicity of the paratylenchus on a plant are kept.

The invention discloses an anaerobic digestion device and application thereof to treating organic wastewater. The anaerobic digestion device comprises a first reactor and a second reactor which are different in size and are sequentially connected in series; the first reactor is divided into a hydrolytic acidification functional area and an anaerobic digestion functional area, which are connected in an overflow manner; the bottom of the first reactor is provided with a water inlet pipe, and inflow speed is controlled by a water inlet pump; the upper side wall of the anaerobic digestion functional area of the first reactor is provided with a reflux pipe and connected with the bottom water inlet pipe of the hydrolytic acidification functional area of the first reactor, and the reflux speed iscontrolled by a reflux pump; the overflow port of the upper reaction area of the first reactor is connected with the bottom water inlet pipe of the second reactor, wastewater processed by the first reactor flows into the second reactor for reprocessing and is then discharged out of the water outlet of the upper reaction area of the second reactor. The anaerobic digestion device is high in processstaring speed, impact resistance and processing efficiency and low in processing cost.

The invention provides a biomass-based activated carbon coated iron carbide three-dimensional porous microbial fuel cell anode material, which is composed of biomass-based activated carbon and iron carbide, wherein iron carbide is distributed in the biomass-based activated carbon and is coated by the biomass-based activated carbon to form micron-sized activated carbon-coated iron carbide particles, and the anode material has a three-dimensional porous network structure consisting of micron and nano composite holes. The invention also provides a microbial fuel cell anode based on the anode material, and preparation methods of the anode material and the anode. According to the invention, the problems of unfavorable attached growth of microorganisms and high modification cost of the existingmodified three-dimensional carbon-based electrode are solved, and the attached growth of an anode biological membrane can be promoted and the electron transfer of an extracellular interface can be accelerated while the cost of the microbial fuel anode is reduced.

The invention discloses plectasin as well as a gene and a preparation method thereof. A nucleotide sequence of the gene of plectasin is shown in SEQ ID No.1 in a sequence table. The invention discloses a gene sequence of plectasin fusion protein, an amino acid sequence, a recombinant expression vector containing the gene of plectasin and a recombinant expression transformant containing a gene coding the plectasin fusion protein, wherein the gene sequence of the plectasin fusion protein contains enzyme digestion sites of His-tag and TEV enzymes. The preparation method of the plectasin comprises the following steps of: cultivating the recombinant expression transformant; separating and purifying the obtained plectasin fusion protein; adding TEV enzyme and carrying out enzyme digestion; and separating and purifying to obtain plectasin protein. By adopting the preparation method of the plectasin, expression quantity of the plectasin is greatly increased, and the plectasin is simpler and easier to separate and purify. The purified plectasin can effectively inhibit growth of gram positive bacteria, bacillus subtilis and methicillin-resistant staphylococcus epidermidis golden yellow staphylococci.