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Method for constructing tissue engineering artificial liver-like tissue

A technology of tissue engineering and artificial liver, which is applied in artificial cell constructs, tissue regeneration, tissue culture, etc., to reduce the loss of cell aggregates, not affect the efficiency of material exchange, and restore the polarity

Active Publication Date: 2018-09-14
GUANGDONG UNISUN BIOTECHNOLOGY CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Hepatocytes are derived from epithelial cells. Primary isolated hepatocytes can adhere to the wall and grow under two-dimensional culture conditions in vitro, but their functional status usually only lasts for about 3-10 days.

Method used

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  • Method for constructing tissue engineering artificial liver-like tissue
  • Method for constructing tissue engineering artificial liver-like tissue
  • Method for constructing tissue engineering artificial liver-like tissue

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Embodiment Construction

[0066] The present invention will be described in further detail below in conjunction with the accompanying drawings and specific embodiments.

[0067] The method for constructing tissue engineering artificial liver-like tissue of the present invention comprises the following steps:

[0068] S1: The hepatic cell suspension is subjected to three-dimensional culture to form cell aggregates.

[0069] Prior to three-dimensional culture, hepatocytes need to be routinely subcultured, that is, cultured on a plate, in order to obtain hepatocytes with better viability; or primary hepatocytes are isolated from fresh liver. When preparing for three-dimensional culture, digest and resuspend the hepatocytes that have reached 80% confluence in plate culture to obtain a hepatocyte suspension in the logarithmic growth phase. The so-called "80% fusion state" generally refers to the state in which there are still a small amount of gaps between the adherent hepatocytes observed by the naked eye...

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Abstract

The invention discloses a method for constructing tissue engineering artificial liver-like tissue. The method comprises the steps as follows: three-dimensional culture of a hepatocyte suspension is performed to form cell clustered-balls; the plurality of the cell clustered-balls are assembled around a central reserved hole to form a clustered-ball microarray; a plurality of the clustered-ball microarrays are closely arranged, packaged and fixed to form artificial liver-like tissue. The method for constructing tissue engineering artificial liver-like tissue is convenient to operate and favourable to large-scale production; and the obtained artificial liver-like tissue is relatively high in bionic degree and has a clinical application prospect.

Description

technical field [0001] The invention relates to the field of tissue engineering, in particular to a method for constructing tissue-engineered artificial liver-like tissue. Background technique [0002] Tissue engineering is a new interdisciplinary discipline developed in the late 1980s. In recent years, with the development of life science, material science and engineering science, researchers have made breakthroughs in the research of in vitro reconstruction of various tissues and organs. Among them, some tissue engineering products such as cartilage and skin have been commercialized. Liver tissue engineering is one of the important research directions in the field of tissue engineering research. Its goal is to construct a transplantable liver tissue or organoid to treat patients with impaired liver function. Today, scientists have simulated the natural liver in the body and successfully constructed a tissue-engineered artificial liver system with certain functions and vit...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/38A61L27/20A61L27/50A61L27/52C12N5/071C12N5/02
CPCA61L27/20A61L27/3804A61L27/3895A61L27/50A61L27/52A61L2430/28C12N5/0671C12N2502/14C12N2513/00C12N2533/76C08L5/12C08L5/04
Inventor 高毅张斌斌彭青李阳李海燕
Owner GUANGDONG UNISUN BIOTECHNOLOGY CO LTD
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