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Method for preparing titania dioxide gel film electrochemical biological sensor

A biosensor, titanium dioxide technology, applied in biochemical equipment and methods, microbial measurement/inspection, scientific instruments, etc., can solve problems such as fixation, and achieve the effects of simplifying the preparation process, avoiding inactivation, and controllable film thickness

Inactive Publication Date: 2004-02-04
NANJING UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

TiO has not been immobilized in sol-gel by molecular self-assembly 2 membranes, and have been used to immobilize biomolecules as reported

Method used

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  • Method for preparing titania dioxide gel film electrochemical biological sensor
  • Method for preparing titania dioxide gel film electrochemical biological sensor
  • Method for preparing titania dioxide gel film electrochemical biological sensor

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Example 1. Self-assembly to form a titanium dioxide film and preparation of a hydrogen peroxide biosensor

[0025] 1. Cut the ITO electrode into 0.5×1.0cm size, then put it in the detergent and ultrasonically clean it twice for 15 minutes, then ultrasonically clean it in absolute ethanol for 10 minutes, and finally ultrasonically clean it in water for 15 minutes. End After that, dry the electrode and let it stand for use.

[0026] 1.1. Soak the ITO electrode in a 5mol / L sodium hydroxide solution for 10 hours, take it out, and rinse it thoroughly with secondary water and dry it with nitrogen;

[0027] 1.2. Immerse the electrode in 100mM butyl titanate solution for 3 minutes, the solvent is a mixture of ethanol and toluene with a volume ratio of 1:1; then put the electrode in absolute ethanol for one minute, and finally soak the electrode in secondary water One minute.

[0028] 2. Immerse the above modified electrode in a 2mg / mL horseradish peroxidase aqueous solution for 30 ...

Embodiment 2

[0031] Example 2. Self-assembly to form a titanium dioxide film and preparation of a hydrogen peroxide biosensor

[0032] 1. Cut the ITO electrode into 0.5×1.0cm size, then put it in the detergent and ultrasonically clean it twice for 15 minutes, then ultrasonically clean it in absolute ethanol for 10 minutes, and finally ultrasonically clean it in water for 15 minutes. End After that, dry the electrode and let it stand for use.

[0033] 1.1. Soak the ITO electrode in a 2mol / L sodium hydroxide solution for 12 hours, take it out and rinse it thoroughly with secondary water, and dry it with nitrogen;

[0034] 1.2. Immerse the electrode in a 200mM butyl titanate solution for 2 minutes, the solvent is a mixture of ethanol and toluene with a volume ratio of 3:1;

[0035] 1.3. Soak the electrode in absolute ethanol for 3 minutes;

[0036] 1.4. Soak the electrode in the secondary water for 3 minutes;

[0037] 2. The modified electrode is immersed in a 2mg / mL horseradish peroxidase aqueous ...

Embodiment 3

[0039] Example 3. Self-assembly to form a titanium dioxide film and preparation of a hydrogen peroxide biosensor

[0040] The cleaning and surface -OH process of the electrode are the same as in Example 1. After -OH, the electrode can be immersed in a 10% silane coupling agent KH550 aqueous solution for 25 minutes to make the electrode surface -NH 2 The remaining steps are the same as in Example 1.

[0041] The obtained biosensor has better performance stability and is stored for one month without deactivation. The choice of experimental conditions is the same

[0042] Example 1.

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Abstract

The method for preparing electrochemical biological transducer with TiO2 gel film includes the following steps: a) preparing TiO2 film, soaking ITO electrode in 2-8 mol / L of sodium hydroxide solution for 5-12 hour, immersing the electrode in 50-300 mM butyl exter titanate for 2-5 minute, immersing the electrode in anhydrous alcohol for 1-3 minute and then in secondary water for 1-3 minute; b) immersing electrode obtained from step a) in aqueous solution of zyme (concentration of 2-4 mg / L) for 30-120 minute. The prepared transducer can hold its activity for 14 days and it can be reused.

Description

1. Technical Field [0001] The invention relates to an electrochemical biosensor prepared by using a self-assembly method to prepare a titanium dioxide gel film on the surface of an electrode and fixing a biologically active substance. 2. Background technology [0002] Biosensors are currently one of the frontier areas of biology and analytical chemistry research. Biosensors use highly selective enzymes, antibodies, antigens and other biologically active substances as receptors to perform highly selective and sensitive analysis and detection of target analytes. In the development of sensors, the immobilization of biological substances on the surface of the carrier is very important. [0003] The current methods for fixing biological substances on the surface of the carrier include adsorption, covalent bonding, cross-linking and embedding. The adsorption method is a simple method of immobilization, usually through the action of electrostatic attraction to immobilize biological subs...

Claims

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Application Information

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IPC IPC(8): C12Q1/26G01N27/327
Inventor 朱俊杰傅瑞玲徐锦忠陈洪渊
Owner NANJING UNIV
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