Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Biological enzyme-catalyzed wall-breaking microalgae grease extraction method

A microalgae oil and bio-enzyme technology, applied in the field of microbial engineering, can solve the problems of low compound enzyme content, difficulty in achieving beneficial effects, and long oil extraction cycle, so as to reduce the amount of enzyme used, reduce extraction costs, shorten The effect of the extraction cycle

Inactive Publication Date: 2019-04-12
HEXI UNIV
View PDF4 Cites 4 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although the method is easy to operate and easy to operate, it is aimed at the efficient oil extraction of oleaginous microalgae, especially photoautotrophic oleaginous microalgae, which reduces the energy input of the microalgae oil extraction process and improves the energy efficiency ratio of microalgae energy. , thereby greatly reducing the comprehensive production cost of microalgae biomass energy; however, the entire oil extraction cycle is too long, and the content of the compound enzyme added cannot be too low, otherwise it will be difficult to achieve the beneficial effect described above, which virtually increases production cost

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] A method for bio-enzyme-catalyzed wall-breaking to extract oil from microalgae, comprising the following steps:

[0027] 1) Take an appropriate amount of chlorella and place it in a solid medium, culture it for 13 days under the conditions of a culture temperature of 25°C and a light intensity of 5500lx, then scrape the microalgae from the surface of the solid medium and put it into warm water, and heat it at 1500rpm High-purity chlorella was obtained by centrifuging at a high speed for 15 minutes;

[0028] 2) Put 500g of high-purity chlorella into purified water, then add a phosphate buffer with a volume of 5% of the volume of pure water and a protease with a weight of 0.5% of the high-purity chlorella, wherein the enzyme activity of the protease is 35000U / g, stir the reaction system and heat to 45°C, continue to heat up to 100°C and boil for 10min after reacting for 0.5h;

[0029] 3) rapidly cooling the boiled reaction solution to 50° C., and adding a compound enzym...

Embodiment 2

[0032] A method for bio-enzyme-catalyzed wall-breaking to extract oil from microalgae, comprising the following steps:

[0033] 1) Take an appropriate amount of chlorella and place it in a solid medium, culture it for 15 days at a culture temperature of 25°C and a light intensity of 5000lx, then scrape the microalgae from the surface of the solid medium and put it into warm water, and heat it at 1500rpm High-purity chlorella was obtained by centrifuging at a high speed for 15 minutes;

[0034] 2) Put 500g of high-purity chlorella into purified water, then add a phosphate buffer with a volume of 5% of the volume of pure water and a protease with a weight of 0.5% of the high-purity chlorella quality, wherein the enzyme activity of the protease is 40000U / g, stir the reaction system and heat to 50°C, continue to heat up to 100°C and boil for 10min after reacting for 0.5h;

[0035] 3) Rapidly cooling the boiled reaction solution to 55° C., and adding a compound enzyme composed of...

Embodiment 3

[0038] A method for bio-enzyme-catalyzed wall-breaking to extract oil from microalgae, comprising the following steps:

[0039] 1) Take an appropriate amount of chlorella and place it in a solid medium, culture it for 15 days at a culture temperature of 25°C and a light intensity of 5000lx, then scrape the microalgae from the surface of the solid medium and put it into warm water, and heat it at 1500rpm High-purity chlorella was obtained by centrifuging at a high speed for 15 minutes;

[0040] 2) Put 500g of high-purity chlorella into purified water, then add a phosphate buffer with a volume of 5% of the volume of pure water and a protease with a weight of 0.5% of the high-purity chlorella, wherein the enzyme activity of the protease is 35000U / g, stir the reaction system and heat to 48°C, continue to heat up to 100°C and boil for 10min after reacting for 0.5h;

[0041] 3) Rapidly cooling the boiled reaction solution to 55° C., and adding 0.8% by weight of high-purity chlorel...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a biological enzyme-catalyzed wall-breaking microalgae grease extraction method. The method comprises the following four steps: microalgae culture centrifugal screening, protease treatment and boiling, ultrasonic treatment after combined enzyme treatment and distillation extraction. Through change of the addition order of enzymes and control on the temperature of a solution, the activity of the enzymes is maximized, the use amount of the enzymes is reduced, and decomposition of whole microalgae cells is accelerated finally through short-term ultrasonic treatment; meanwhile, the decomposition products is isolated from grease on microalgae, and subsequent grease extraction is facilitated; and not only can the extraction rate of internal grease of the microalgae be improved, but also the use amount of the enzymes is reduced, the extraction cycle is shortened, and the extraction cost is reduced greatly.

Description

technical field [0001] The invention relates to the technical field of microbial engineering, in particular to a method for extracting oil from microalgae by catalyzing bio-enzyme breaking walls. Background technique [0002] Algae biofuels are an important part of the global renewable resource system. As the main form of biofuel, the oil in microalgae is biodiesel prepared by using the oil in microalgae to react under catalyst conditions. Compared with traditional biofuels, algae biofuel has more advantages. Among them, some microalgae are most useful because their stem cells are rich in microalgae oil of more than 70%, which is the best raw material for subcritical biotechnology to synthesize biodiesel, and is an ideal renewable energy source. [0003] At present, the techniques for extracting the internal oil of microalgae mainly include traditional physical methods such as mechanical crushing, ultrasonic wave, colloid mill, and biological enzymatic hydrolysis methods. T...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C11B1/02C11B1/10C11B3/12
CPCC11B1/02C11B1/025C11B1/106C11B3/12
Inventor 王丽娟罗光宏陈天仁杨宋琪
Owner HEXI UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com