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Kit for testing pesticide residue rapidly and use method thereof

A technology for detecting kits and pesticide residues, which can be used in the preparation of test samples, material analysis by observing the impact on chemical indicators, and analysis by making materials undergo chemical reactions. It can solve the problem of inaccurate pesticide varieties and Quantitative, can not determine the type of pesticides and other issues, to achieve the effect of low cost, easy production, high sensitivity

Inactive Publication Date: 2005-10-26
NANJING AGRICULTURAL UNIVERSITY +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The enzyme inhibition method and the rapid test instrument and quick test card developed based on the principle of the enzyme inhibition method are usually aimed at organophosphorus and carbamate pesticides. Carbamate pesticides, etc.

Method used

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  • Kit for testing pesticide residue rapidly and use method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] Example 1 Type 20 Pesticide Residue Rapid Detection Plate Colorimetric Kit

[0042] One ice bottle, 20cm in diameter and 30cm in height, containing 1 part (1mg) of esterase freeze-dried powder that can detect 20 samples. 1 carton, 11cm long x 8cm wide x 13cm high, inside: 1 white ceramic plate, 12cm long x 10cm wide x 1cm high, with 12 holes in total. One 100mL beaker, one 10mL beaker, one 25mL measuring cylinder, one glass rod. 5 droppers. Extraction reagent 1 bag, including 2.1gNa 2 HPO 4 , 0.1g KH 2 PO 4 and 1g Triton X-100 in a plastic bag. 1 bottle of substrate, 0.0125% thioacetylcholine iodide solution, 2 mL. Chromogen 1 # 1 bottle, developer 2 # 1 bottle, 2mL each.

[0043] The following reagents can meet the rapid detection of organophosphorus and carbamate pesticide residues in 20 samples:

[0044] Extraction reagents include 2.1gNa 2 HPO 4 , 0.1g KH 2 PO 4 and 1g Triton X-100. It can be put in a plastic bag, or in a 100mL volumetric flask or a ...

Embodiment 2

[0062] Example 2 Type 100 Pesticide Residue Rapid Detection Plate Colorimetric Kit

[0063] 1 ice bottle, 15cm in diameter and 30cm in height, containing 1 part (5mg) of esterase freeze-dried powder that can detect 100 samples. 1 carton, 11cm long x 8cm wide x 13cm high, inside: 2 white ceramic plates, 12cm long x 10cm wide x 1cm high, with 12 holes in total. One 100mL beaker, two 10mL beakers, one 25mL measuring cylinder, and one glass rod. 5 droppers. 5 bags of extraction reagents, each containing 2.1gNa 2 HPO 4 , 0.1g KH 2 PO 4 and 1g Triton X-100 in a plastic bag. 1 bottle of substrate, 0.0125% thioacetylcholine iodide solution, 10 mL in total. Chromogen 1 # 1 bottle, developer 2 # 1 bottle, each 10mL.

[0064] The following reagents can meet the rapid detection of organophosphorus and carbamate pesticide residues in 100 samples:

[0065] Extraction reagents include 10.5g Na 2 HPO 4 , 0.5g KH 2 PO 4 and 5g Triton X-100. It can be placed in a plastic bag, or...

Embodiment 3

[0069] Embodiment 3 minimum detection limit determination

[0070] Pesticide varieties

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PUM

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Abstract

The invention relates to the pesticide remains fast detecting reagent box and its using method. The reagent comprises the extracting reagent, the base material, the color-showing agent and enzyme. It judges the whether the pesticide remains surpass the standard amount through the color-comparison reaction. The manufacturing of the box is very simple, and cost is very low and the detection process is fast.

Description

1. Technical field [0001] The invention relates to a rapid detection kit for pesticide residues and its usage. It is dedicated to the rapid detection of organophosphorus and carbamate pesticide residues in agricultural products, food, water, soil, poisonous organisms, especially grain, vegetables, fruits, Chinese medicinal materials and meat. 2. Background technology [0002] With the rapid development of my country's industry and the strategic adjustment of agricultural and industrial structures, especially the transformation of cultivation methods, the occurrence and harm of harmful organisms such as pest weeds, which were originally secondary or even quarantine, are becoming more and more serious, and drug resistance continues to rise. , leading to an increase in the amount of pesticides and the frequency of application. The abuse of highly toxic and high-residue pesticides has been repeatedly prohibited. Some use pesticides not in accordance with the regulations, and som...

Claims

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Application Information

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IPC IPC(8): G01N21/79
Inventor 杨春龙张德琴胡秋辉王海峰杨红陈守勇李燕安辛欣
Owner NANJING AGRICULTURAL UNIVERSITY
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