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Antibody against 5-hydroxymethyl furfural

A hydroxymethylfurfural and antibody technology, applied in the biological field, can solve the problems of high cost, complicated and complicated operation, slow analysis speed and the like, and achieve the effects of few reaction steps, simple synthesis method and easy acquisition.

Inactive Publication Date: 2013-05-01
TIANJIN UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although these traditional physical and chemical analysis methods are highly sensitive and accurate, they are generally cumbersome and complicated to operate, high in cost, and slow in analysis speed, so it is difficult to meet the needs of actual analysis. Therefore, it is urgent to develop a simple, fast, sensitive analysis techniques

Method used

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  • Antibody against 5-hydroxymethyl furfural
  • Antibody against 5-hydroxymethyl furfural
  • Antibody against 5-hydroxymethyl furfural

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Embodiment 1

[0033] 1. Hapten Synthesis

[0034] Hapten molecular weight of the present invention is 226, and molecular structural formula is:

[0035]

[0036] The specific preparation method is: add 144.9mg (1.15mmol) of 5-hydroxymethylfurfural into a small amount of newly prepared tetrahydrofuran solution to dissolve it, then add 140.3mg (1.15mmol) of 4-lutidine, triethylamine (2.3 mmol) 0.317ml, succinic anhydride (2.3mmol) 230mg, stirred and reacted overnight (about 9 hours) in an oil bath at 60°C. The solvent was removed under reduced pressure, and the residue was dissolved with methanol / dichloromethane / glacial acetic acid (1:9.5:0.05 V / V / V). The primary product was purified by column chromatography with methanol / dichloromethane / glacial acetic acid (1:9.5:0.05 V / V / V). The obtained product was collected and evaporated under vacuum to obtain a relatively pure light yellow hapten.

[0037] Use 10g of 200-300 mesh silica gel for packing the column, and add 1.0g of the same specific...

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Abstract

The invention relates to preparation of a semiantigen, an artificial antigen and an antibody with a basic structure of 5-hydroxymethyl furfural. According to the invention, the antibody with good specificity to 5-hydroxymethyl furfural is prepared by using an easy and convenient method. The antibody is prepared through the following steps: synthesizing the semiantigen from 5-hydroxymethyl furfural and succinic anhydride; respectively connecting the semiantigen with KLH and HRP to synthesize the artificial antigen and an enzyme-labeled antigen; and subjecting the artificial antigen to animal immunization, blood drawing, separation of antiserum and purification so as to prepare the antibody. The antibody is stable and has good specificity and sensitivity; the synthetic method for the antibody is simple; and the antibody can be used for rapid immunodetection of 5-hydroxymethyl furfural in foodstuffs and has a good application prospect.

Description

technical field [0001] The present invention relates to selecting a compound having -COOH and possibly containing the original structure of 5-hydroxymethylfurfural as the hapten of 5-hydroxymethylfurfural, and making the hapten into an antigen to generate antibodies; and Hapten-like, antigen synthesis and antibody preparation methods. The invention belongs to the field of biotechnology. Background technique [0002] In recent years, with the improvement of living standards, harmful carcinogens in fried foods have attracted widespread attention. 5-Hydroxymethylfurfural is a ubiquitous substance in fried foods. It is a furfural compound with a furan ring structure, mainly produced by decomposing hexose sugar under acid and heating conditions. It widely exists in foods containing carbohydrates, such as bread, potato chips, apple juice, instant coffee, baby dairy products, honey, milk, etc. Relevant studies have shown that 5-HMF may cause and promote abnormal growth of colon...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K14/765C07K16/06C07D307/46G01N33/535
Inventor 方国臻张燕王俊平王硕吕燕颜
Owner TIANJIN UNIV OF SCI & TECH
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