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Dopamine content detection method

A dopamine and content technology, applied in fluorescence/phosphorescence, material excitation analysis, etc., can solve the problem that the application research of metal nanoparticle composite ICP materials has not been reported, and achieve the effect of easy on-site measurement, small sample demand and high sensitivity

Active Publication Date: 2014-08-13
INST OF CHEM CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

So far, the properties of this metal nanoparticle composite ICP material and its application in in vivo analytical chemistry have not been reported.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0051] Embodiment 1, preparation AgZMZ

[0052] AgNO at a concentration of 12.9mM 3 The ethanol solution of the infinite coordination polymer ZMZ and the ethanol dispersion of the infinite coordination polymer ZMZ (the dosage ratio of ZMZ and ethanol is 1mg: 1mL) were mixed and stirred overnight at room temperature to carry out the reduction reaction, and the metal nanoparticle composite AgZMZ material was obtained after the reaction was completed;

[0053] Among them, the infinite coordination polymer ZMZ can be prepared according to the steps reported in the literature (Adv. Mater. The solution and the dimethyl sulfoxide solution of zinc salt dihydrate zinc acetate dihydrate with a concentration of 0.023mM are mixed by super-vibration in an equimolar ratio (that is, the molar ratio of the ligand MSB shown in formula II to the zinc salt), and then left to stand. Diethyl ether was then added dropwise. After 1 hour, a yellow precipitate was generated, which was washed with di...

Embodiment 2

[0059] Embodiment 2, AgZMZ to the fluorescence response analysis performance of different concentrations of dopamine artificial cerebrospinal fluid samples

[0060] A series of dopamine artificial cerebrospinal fluid standards with different molar concentrations were added to the metal nanoparticle composite ICP material obtained in Example 1, that is, the ethanol dispersion of AgZMZ, and the final concentrations of dopamine added were 0nM, 10nM, 20nM, 40nM, 80nM, 100nM, measured with a fluorescence instrument, taking the molar concentration of dopamine in the dopamine artificial cerebrospinal fluid standard as the abscissa, and the fluorescence response value at 365nm as the ordinate (excitation wavelength: 287nm), draw a standard curve, such as Figure 5 As shown, the corresponding unary linear equation is Y=49.6936+11.525X, wherein, Y is the fluorescence response value, unitless, X is the molar concentration of dopamine, the unit is nM; the linear range of the standard curve...

Embodiment 3

[0065] Example 3. Fluorescence analysis performance of AgZMZ on various interfering substances that may exist in the rat brain.

[0066] The fluorescence analysis performance of AgZMZ on various interfering substances (glutathione, lactic acid, glucose, uric acid, glutamic acid, glycine, serotonin and dihydroxyphenylacetic acid) that may exist in the rat brain was analyzed as follows:

[0067] Add 10 μL of prepared dopamine, glutathione, lactic acid, glucose, uric acid, glutamic acid, glycine, 5-hydroxytryptamine and dihydroxyphenylacetic acid to 2.0 mL of AgZMZ ethanol suspension with a concentration of 1 mg / mL. In the artificial cerebrospinal fluid solution, the final concentration of the added interfering substances was 10 μM, which was directly measured with a fluorescence instrument.

[0068] Figure 6 It is the fluorescence response of AgZMZ to a variety of interfering substances that may exist in the rat brain. The fluorescence emission wavelength is taken as the absci...

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Abstract

The invention discloses a metal nanoparticle composite ICP (inductively coupled plasma) material and application thereof in dopamine content detection. The metal nanoparticle composite ICP material is an infinite coordination polymer comprising an Ag nanoparticle-modified repeating structural unit shown as formula I. By use of highly-sensitive and highly-selective fluorescent recognition functions of the surface modified rough Ag nanoparticle infinite coordination polymer ICP material on dopamine, direct fluorescence detection of the dopamine content in rat brain dialysis fluid can be realized. The method has the advantages of high sensitivity, good selectivity, fast response, low cost, less sample demand, and the like, and more importantly, the method has the advantages of simple operation and high stability, and can be applied to the determination of dopamine basic values in living body.

Description

technical field [0001] The invention relates to a method for measuring dopamine content. Background technique [0002] Dopamine is an important neurotransmitter in the brain. It is mainly responsible for the brain's desire and feeling, and transmits information of excitement and happiness. The feeling of love is actually the result of a large amount of dopamine in the brain. It is also related to addiction. Both smoking and drug use can increase the secretion of dopamine, making addicts feel happy and excited. Insufficient or imbalanced dopamine can make people unable to concentrate or lose the ability to control muscles. In severe cases, it can lead to involuntary trembling of hands and feet, and even Parkinson's disease. Therefore, the determination of the content of dopamine in the brain has important physiological and pathological significance. [0003] The static content of dopamine in the brain is very low (less than 100nM), and there are many interfering substances....

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08L85/00C08K3/08G01N21/64
Inventor 毛兰群任琳杨丽芬于萍
Owner INST OF CHEM CHINESE ACAD OF SCI
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