31 results about "Dihydroxyphenylacetic acid" patented technology

The invention provides a preparation method of caffeic acid, which comprises the following steps: step one: 3,4-dihydroxyphenylacetic acid is dissolved in polyethylene glycol 200 mixed with an entrainer, hydrocyanic acid is introduced to carry out the addition reaction under the condition of radiation of microwaves with power of 1,000 to 1,800 W, and then supercritical carbon dioxide is added to extract an addition product; step two: the addition product is hydrolyzed, separated and purified to obtain a hydrolysis product; and step three: the hydrolysis product is dehydrated to obtain caffeic acid. The method has the advantages of mild condition and less side reaction, is favorable to environmental protection, simplifies the production process, shortens the reaction time, reduces the production cost and the production period greatly, is simple and feasible under the industrial production condition, has more than 90% of yield, and is easy to achieve the industrial batch production; and the produced waste is less and easy to treat. Meanwhile, 3,4-dihydroxyphenylacetic acid can be prepared from pyrocatechol through a simple process route, so that the cost is reduced greatly.

The invention discloses a production method of vertical plywood capable of preventing delamination and seepage. The production method comprises the steps that aminated lignin is dissolved in DMF, PEG200 and neopentyl glycol are added for stirring and dissolving, isophorone diisocyanate is then added for stirring uniformly, then bismuth laurate is dropwise added into the mixture, and a first material is obtained by reacting; chitosan is dissolved in hydrochloric acid; 3,4-dihydroxyphenylacetic acid is added to obtain a mixed solution; EDC / NHS is dissolved in an ethanol aqueous solution and thendropwise added into the mixed solution for reaction, dialyzing is carried out in hydrochloric acid with pH of 5, and freeze-drying is carried out to obtain a second material; sesame protein is addedinto a urea aqueous solution for stirring, and then zinc oxide is added for stirring to obtain a third material; and the first material and the second material are added into the third material to beuniformly mixed, then a strong oxidant is added to be uniformly mixed, the surface of a single plate is coated with the mixture, then adjacent single plates are glued and stacked, a plurality of layers of plate blanks are assembled, and hot pressing is carried out to obtain the vertical plywood capable of preventing delamination and seepage.

The invention belongs to the technical field of plant genetic engineering, and particularly relates to a wheat stripe rust resistance related metabolite and a synthesis related gene and application thereof. In the combined analysis of metabolome and transcriptome of wheat infected by stripe rust, 2, 5-dihydroxyphenylacetic acid (HGA) and a synthetic related gene TaHPD thereof are induced to be up-regulated, and the resistance of wheat and corn to rust diseases can be improved and the germination of uredospores of the stripe rust can be inhibited by externally spraying the HGA. Gene silencing and TaHPD overexpression also prove that the change of the HGA content affects the disease resistance of plants. The compound HGA provided by the invention can be processed into a prevention and control agent to realize green prevention and control of crop diseases.

The invention discloses a method of preparing an o-phenol compound by an enzyme method. The method comprises the step of performing a reaction for 1-7h at 15-40 DEG C to obtain the o-phenol compound by taking a carrier-free immobilized tyrosinase as a catalyst, a monophenol compound as a primer, dimethyl sulfoxide as a cosolvent and L-ascorbic acid as a reducing agent. According to the method disclosed by the invention, by taking the carrier-free immobilized tyrosinase as the biological catalyst and monophenol compound as the primer, the o-phenol compound is synthesized. The method has the advantages of being mild in reaction condition, short in period, high in specificity of reaction, simple in process step, safe to operate and the like. The invention provides a novel method of greenly producing piceatannol, 3'-hydroxyl pterostilbene and 3,4-dihydroxyphenylacetic acid.

The invention discloses a method for enzymatically preparing o-phenolic compounds, which uses carrier-free immobilized tyrosinase as a catalyst, a single phenolic compound as a substrate, dimethyl sulfoxide as a cosolvent, and L-ascorbic acid As a reducing agent, react at 15-40°C for 1-7 hours to obtain o-phenolic compounds. The present invention innovatively uses carrier-free immobilized tyrosinase as a biocatalyst and single phenolic compound as a substrate to synthesize o-phenolic compounds, which has the advantages of mild reaction conditions, short cycle, high reaction specificity, simple process steps, The advantages of safe operation provide a new method for the green production of piceatanol, 3'-hydroxy pterostylbene and 3,4-dihydroxyphenylacetic acid.

The invention discloses dopamine and dopamine metabolite based molecularly imprinted magnetic nanospheres as well as a preparation method and an application thereof. The method comprises the followingsteps: synthesizing carboxyl-functionalized magnetic nanospheres with a hydrothermal method; adding the carboxyl-functionalized magnetic nanospheres and two epinephrine metabolites to a phosphate buffer solution in a certain ratio to obtain a dual-template-carrier complex; stirring a separation product, functional monomer hyperbranched polyethyleneimine and crosslinking agent glutaraldehyde in the phosphate buffer solution for polymerization to obtain a polymer; separating a solid polymer out by externally applying a magnetic field; finally, performing reflux elution on the solid polymer, andperforming vacuum drying to obtain the dopamine and dopamine metabolite based molecularly imprinted magnetic nanospheres. The preparation method is simple, conditions are mild, and the prepared molecularly imprinted magnetic nanospheres are uniform in particle size, high in stability and good in water solubility, and can realize selective enrichment and detection of dopamine and 3,4-dihydroxyphenylacetic acid in complicated matrix of human fluid simultaneously and rapidly.

The invention discloses the application of berberine dimer analog derivatives in the preparation of medicines for preventing and treating Parkinson's disease. The structural formula of the berberine dimer analog derivative is shown in formula I. In the present invention, it is found that berberine dimer analogue derivatives or pharmaceutically acceptable salts thereof show better pharmacodynamic activity than the parent compound berberine on the in vitro cell model of Parkinson's disease, and it can obviously Improve the behavior of Parkinson's disease model mice, increase the number of dopaminergic neurons in the substantia nigra compacta, and increase the levels of dopamine and 3,4-dihydroxyphenylacetic acid in the striatum. Therefore, berberine dimer analog derivatives or pharmaceutically acceptable salts thereof can be made into related medicines for preventing and treating Parkinson's disease.

The present invention provides applications of 3,4-Dihydroxyphenylacetic acid (DOPAC) or medically acceptable salt, solvolyte, or polycrystal thereof in preparing drugs for preventing and / or curing tumors. Experiments in vivo and vitro indicate that DOPAC can suppress multiple types of tumors such as lung cancer, breast cancer, cervical carcinoma, nephroblastoma, nasopharyngeal carcinoma, laryngeal carcinoma, and neuroblastoma, and has broad-spectrum antitumor functions. This type of compounds are derived from natural drugs, and hopefully provide new choices for clinical treatment.

The invention discloses a production method of an anti-delamination and anti-seepage water vertical plywood, comprising: dissolving aminated lignin in DMF, adding PEG200 and neopentyl glycol, stirring and dissolving, and adding isophorone diisocyanate Stir evenly, then add bismuth laurate dropwise to it to react to obtain the first material; dissolve chitosan in hydrochloric acid, and then add 3,4-dihydroxyphenylacetic acid to obtain a mixed solution; then dissolve EDC / NHS in ethanol aqueous solution , then added dropwise into the mixed solution for reaction, then dialyzed in hydrochloric acid with a pH of 5, and freeze-dried to obtain the second material; adding sesame protein into the urea aqueous solution and stirring, then adding zinc oxide and stirring to obtain the third material; The first material and the second material are added to the third material and mixed evenly, then a strong oxidant is added to mix evenly, and coated on the surface of the veneer, and then adjacent veneers are glued, stacked, multi-layered, and hot-pressed to obtain anti-delamination and anti-corrosion Water seepage vertical plywood.

The invention discloses application of kurarinone in preparation of a medicine for preventing and treating Parkinson's disease, after 1-methyl-4-phenyl-1, 2, 3, 6-tetrahydropyridine hydrochloride (MPTP) is adopted for modeling, the athletic ability of a PD mouse is reduced, the levels of dopamine (DA) and metabolites 3, 4-dihydroxyphenylacetic acid (DOPAC) and homovanillic acid (HVA) in the brain are reduced, and the level of tyrosine hydroxylase (TH) is reduced. Kurarinone is given to inhibit the activity of soluble epoxide hydrolase (sEH) and improve the content of dopamine (DA) and metabolites of 3, 4-dihydroxyphenylacetic acid (DOPAC) and homovanillic acid (HVA) in the brain, so that MPTP induced PD is improved.

The invention discloses a modified water-soluble chitosan flocculating agent and a preparation method thereof. The preparation method comprises the following steps: a carboxymethyl reaction step: dispersing chitosan into a mixed solution of isopropanol and sodium hydroxide, stirring for alkalization so as to obtain a reaction mixture, dissolving monochloro acetic acid into isopropanol to obtain asolution, dropwise adding the solution into the reaction mixture, reacting for 3-5 hours, adding an ethanol water solution to terminate the reaction, washing, and drying, so as to obtain carboxymethylchitosan; and a cross-linking reaction step: dissolving carboxymethyl chitosan into hydrochloric acid, adjusting the pH value to 5, dissolving 3,4-dihydroxyphenylacetic acid into a certain amount ofwater to obtain a solution, adding the solution into a carboxymethyl chitosan solution, dissolving a mixture of EDC and NHS into mixed liquid of water and ethanol to obtain a solution, adding the solution into a reaction solution, adding hydrogen peroxide into the reaction solution, stirring to maintain the pH value of the reaction solution at 4.5-5.0, and reacting at the room temperature for 9-11hours, so as to obtain the modified water-soluble chitosan flocculating agent. The flocculating agent disclosed by the invention has good water solubility and flocculating property.

The invention provides a preparation method of caffeic acid, which comprises the following steps: step one: 3,4-dihydroxyphenylacetic acid is dissolved in polyethylene glycol 200 mixed with an entrainer, hydrocyanic acid is introduced to carry out the addition reaction under the condition of radiation of microwaves with power of 1,000 to 1,800 W, and then supercritical carbon dioxide is added to extract an addition product; step two: the addition product is hydrolyzed, separated and purified to obtain a hydrolysis product; and step three: the hydrolysis product is dehydrated to obtain caffeic acid. The method has the advantages of mild condition and less side reaction, is favorable to environmental protection, simplifies the production process, shortens the reaction time, reduces the production cost and the production period greatly, is simple and feasible under the industrial production condition, has more than 90% of yield, and is easy to achieve the industrial batch production; and the produced waste is less and easy to treat. Meanwhile, 3,4-dihydroxyphenylacetic acid can be prepared from pyrocatechol through a simple process route, so that the cost is reduced greatly.

The invention provides a high-efficiency separation method of polyphenol components in dandelion, which adopts a mode of combining pH zone counter-current chromatography (pH-ZRCCC) and high-speed counter-current chromatography (HSCCC) to separate six polyphenol compounds from dandelion. In pH-ZRCCC separation, a solvent system is selected as ethyl acetate-acetonitrile-water (4: 1: 5, v / v / v), trifluoroacetic acid (10mM) is added into an upper phase as a stationary phase, and ammonia water (10mM) is added into a lower phase as a mobile phase. Caffeic acid, hydroxycinnamic acid and a mixture A are separated from the dandelion ethyl acetate crude extract; hSCCC is further utilized, and a solvent system is selected as petroleum ether-ethyl acetate-methanol-water (1: 4: 1: 4, v / v / v / v). 1-O-caffeoylglycerol, 3, 4-dihydroxyphenylacetic acid, protocatechuic acid and p-hydroxyphenylacetic acid are obtained through separation from 400 mg of the mixture A. The method has the advantages of large preparation amount and good reproducibility, and is suitable for separation and purification of dandelion polyphenol compounds.

The invention discloses a porous concrete waterproofing agent and a preparation method thereof. The raw materials of each component of the waterproofing agent include: by weight, 25-40 parts of modified gel, 4-6 parts of oxidizing agent, and 5-9 parts of crosslinking agent 3-5 parts, photoinitiator; The raw materials of each component of the modified gel include: by weight, 30-40 parts of pretreated microspheres, 25-30 parts of 3,4-dihydroxyphenylacetic acid, N- Hydroxysuccinimide 15‑20 parts, 1‑(3‑dimethylaminopropyl)‑3‑ethylcarbodiimide hydrochloride 15‑20 parts, sodium alginate 18‑25 parts, propyne 10‑ 14 servings. The invention discloses a porous concrete waterproofing agent and a preparation method thereof. The process design is simple, and the component distribution ratio is reasonable. Chitosan and sodium alginate are modified, and Michael addition reaction and chemical click reaction are used to form a dense waterproof agent. The dual-gel network can not only effectively improve the waterproof and anti-seepage performance of concrete, but also self-repair the waterproofing agent. It can be widely used in concrete construction and has high practicability.

The invention discloses a new method for preparing high-purity hydroxytyrosol, which belongs to the technical field of pharmaceutical chemical synthesis. The specific steps of the method are: uniformly mixing lithium aluminum hydride with a solvent, and then adding 3,4-dihydroxyphenylacetic acid in batches Carry out the reaction, place the reaction solution at 0°C after the reaction is completed, slowly add water and hydrochloric acid solution dropwise, extract with ethyl acetate after dropping, combine the organic phases, wash with aqueous sodium bicarbonate solution, and dry over anhydrous magnesium sulfate After that, the salt was removed by suction filtration, and the solvent was evaporated under reduced pressure to obtain a light yellow oily substance which was hydroxytyrosol. The beneficial effects are: the raw materials of the new preparation method of the present invention are easy to obtain, the cost is low, the operation is simple, and 3,4-dihydroxyphenylacetic acid can be reduced to hydroxytyrosol in one step, the reaction rate is fast, the yield of the target product is high, and no column chromatography is required Purification can produce high-purity hydroxytyrosol, which is suitable for industrial production.

The invention discloses a preparation method and a product of tin dioxide doped hydrosol and application thereof in self-cleaning of cotton fabrics. The preparation method comprises the following steps: weighing tin powder and crystalline tin tetrachloride; adding distilled water for dissolving, stirring and then adding into a stainless steel reaction kettle with a polytetrafluoroethylene lining;baking in an oven at the high temperature of 180 DEG C for 24 h, and cooling for 5 h to remove a supernatant; placing precipitates on the lower layer in a centrifuge tube and centrifuging at the speedof 10000 rpm for 5 min; washing the precipitates once, and adding absolute ethyl alcohol for washing three times; centrifuging for 5 min, taking the precipitates and drying the precipitates in the oven; grinding to obtain tin dioxide doped crystals; weighing 8 mg of 3, 4-dihydroxyphenylacetic acid, dissolving the 3, 4-dihydroxyphenylacetic acid into 50mL of distilled water, carrying out ultrasonic treatment for 5 min, stirring by a magnetic stirrer for 10 min, adding 0.5g of the tin dioxide doped crystals, and stirring for 10 min to obtain tin dioxide doped hydrosol which is used for finishing the cotton fabrics. While the cotton fabrics have remarkable self-cleaning performance, the force of binding of nanoparticles and the fabrics can be increased, and the fastness is improved.