31 results about "Dihydroxyphenylacetic acid" patented technology

The invention discloses a mussel-imitated underwater high-viscosity hydrogel preparation method, which comprises: (1) dissolving chitosan in acetic acid to prepare a chitosan acetic acid solution; (2)adding 3,4-dihydroxyphenylacetic acid, EDC and NHS into the solution prepared in the step (1), adjusting the pH value, and carrying out a reaction for a certain time; (3) dialyzing the solution obtained in the step (2) in water with the pH value of 5, taking the trapped fluid, and carrying out freeze-drying; (4) preparing the sample obtained in the step (3) into a solution; (5) dissolving glucanin water, adding an oxidant, reacting for a certain time in a dark place, and then adding ethylene glycol to terminate the reaction; (6) dialyzing the solution obtained in the step (5) in water, taking the trapped fluid, and carrying out freeze-drying; (7) preparing the sample obtained in the step (6) into a solution; and (8) mixing the solutions obtained in the step (4) and the step (7) to obtainthe hydrogel. According to the preparation method provided by the invention, the prepared hydrogel can be used as an underwater adhesive.

The invention discloses dopamine and dopamine metabolite based molecularly imprinted magnetic nanospheres as well as a preparation method and an application thereof. The method comprises the followingsteps: synthesizing carboxyl-functionalized magnetic nanospheres with a hydrothermal method; adding the carboxyl-functionalized magnetic nanospheres and two epinephrine metabolites to a phosphate buffer solution in a certain ratio to obtain a dual-template-carrier complex; stirring a separation product, functional monomer hyperbranched polyethyleneimine and crosslinking agent glutaraldehyde in the phosphate buffer solution for polymerization to obtain a polymer; separating a solid polymer out by externally applying a magnetic field; finally, performing reflux elution on the solid polymer, andperforming vacuum drying to obtain the dopamine and dopamine metabolite based molecularly imprinted magnetic nanospheres. The preparation method is simple, conditions are mild, and the prepared molecularly imprinted magnetic nanospheres are uniform in particle size, high in stability and good in water solubility, and can realize selective enrichment and detection of dopamine and 3,4-dihydroxyphenylacetic acid in complicated matrix of human fluid simultaneously and rapidly.