A method for evaluating neurodevelopmental toxicity of perfluorinated compounds using zebrafish

A perfluorinated compound and neurodevelopmental technology, applied in the field of toxicology detection, can solve the problems of subjective judgment deviation of deformity, error in experimental results, and zebrafish larvae are not considered, and achieves reduction of experimental cost, efficient data processing, and analysis. clear results

Active Publication Date: 2020-06-26
CHINESE RES ACAD OF ENVIRONMENTAL SCI
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Problems solved by technology

[0005] Hu Qin's paper "Studies on the Toxic Effects of Perfluorooctane Sulfonate (PFOS) on Zebrafish Embryo Development and Adult Fish" judged the effects of perfluorinated compounds on zebrafish embryos from the mortality rate, hatching rate, heart rate, and deformity rate of zebrafish embryos. Embryo toxicity, but this method does not take into account the individual differences in the development of zebrafish juveniles, as well as the subjective bias in judging abnormalities, resulting in large errors in experimental results

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  • A method for evaluating neurodevelopmental toxicity of perfluorinated compounds using zebrafish
  • A method for evaluating neurodevelopmental toxicity of perfluorinated compounds using zebrafish
  • A method for evaluating neurodevelopmental toxicity of perfluorinated compounds using zebrafish

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[0045] In order to make the objectives, technical solutions and advantages of the present invention clearer, the present invention will be further described in detail below with reference to the embodiments. It should be understood that the specific embodiments described herein are only used to explain the present invention, but not to limit the present invention.

[0046] A method for evaluating neurodevelopmental toxicity of perfluorinated compounds using zebrafish, comprising the following steps:

[0047] 1) Using chromatographically pure DMSO as a solvent, PFHxA (perfluorohexanecarboxylic acid) was prepared into a high-concentration compound stock solution with a concentration of 30 mg / mL, and stored at 4°C in the dark;

[0048] 2) Dilute the pre-configured high-concentration stock solution by gradient dilution of the embryo culture solution to obtain culture solutions with concentrations of 0.48 mg / L, 2.4 mg / L and 12 mg / L, respectively. The concentration of DMSO is contr...

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Abstract

The invention discloses a method for evaluating neurodevelopment toxicity of perfluorinated compounds by utilizing zebra fishes. Brain neuron specific fluorescent protein expression quantity (microscopic observation) of the transgenic zebra fishes after acute exposure of the perfluorinated compounds, motion behaviors of wide zebra fish fries, neurotransmitters (acetylcholine, dopamine, 3,4-dihydroxyphenylacetic acid, aminobutyric acid and hydroxylamine, neurodevelopment related genes (chrna7, ache, mbp, a1-tubulin, shha, elavl3, gap43syn2a, gfap, bdnf, TH2, htr1ab, htr1b, htr2a, htr1aa, htr5a,DAT and Nr4a2b) and a protein expression level are measured. The transgenic zebra fishes are combined with the wild zebra fishes, a relation between the indexes and the neurodevelopment toxicity is elaborated, and the neurodevelopment toxicity level of the perfluorinated compounds can be comprehensively and accurately evaluated.

Description

technical field [0001] The invention relates to a method for evaluating the neurodevelopmental toxicity of perfluorinated compounds by using zebrafish, and belongs to the technical field of toxicology detection. Background technique [0002] Perfluorinated compounds (PFCs) are a new class of organic pollutants, mainly including perfluorocarboxylic acids (PFCAs) and perfluorinated sulfonic acids (PFSAs). Since it was developed by 3M in the 1950s, it has been widely used due to its remarkable hydrophobicity and oleophobicity, as well as good surface activity and stability (Ahrens, 2011). However, the development, production, use and disposal of a large number of PFCs-containing commodities make PFCs migrate into environmental media and organisms through various ways, posing a threat to the ecological environment and human health. Toxicological studies have confirmed that PFCs have liver toxicity, cardiovascular toxicity, thyroid toxicity, nervous system toxicity, immune syste...

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Application Information

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IPC IPC(8): C12Q1/6851C12Q1/02G01N33/50
CPCG01N21/6458G01N27/447G01N30/02G01N30/06G01N33/5014
Inventor 国晓春卢少勇郑丙辉张胜男刘晓晖李广宇
Owner CHINESE RES ACAD OF ENVIRONMENTAL SCI
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