Efficient separation method of polyphenol components in dandelion

A separation method, the technology of dandelion, applied in the field of chemical analysis, can solve the problem of less separation, and achieve the effect of simple operation, shortening the time of separation and preparation, and efficient preparation method

Active Publication Date: 2022-04-05
QIANFOSHAN HOSPITAL OF SHANDONG
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

At present, there are relatively few research reports on the separation of dandelion polyphenols by countercurrent chromatography

Method used

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  • Efficient separation method of polyphenol components in dandelion
  • Efficient separation method of polyphenol components in dandelion
  • Efficient separation method of polyphenol components in dandelion

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Embodiment 1

[0023] 1 Materials and methods

[0024] 1.1 Main raw materials

[0025] Dandelion was purchased from the local medicinal materials market in Shandong, and was identified as Taraxacum mongolicum Hand.-Mazz. by experts; n-butanol, n-hexane, methanol, absolute ethanol, ethyl acetate, petroleum ether, ammonia water, trifluoroacetic acid and other reagents were used in the experiment. It is analytically pure; methanol used for HPLC analysis is chromatographically pure.

[0026] 1.2 Instruments and equipment

[0027] The high-speed countercurrent chromatography system is equipped with an infusion pump, a cryogenic constant temperature tank, an ultraviolet detector, and a portable recorder; other equipment includes a pH meter, a rotary evaporator, a high-performance liquid chromatography, and a nuclear magnetic resonance spectrometer.

[0028] 2 Experimental methods

[0029] 2.1 Extraction of dandelion polyphenols

[0030] Take 1 kg of dandelion and grind it, use 60% ethanol, the...

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Abstract

The invention provides a high-efficiency separation method of polyphenol components in dandelion, which adopts a mode of combining pH zone counter-current chromatography (pH-ZRCCC) and high-speed counter-current chromatography (HSCCC) to separate six polyphenol compounds from dandelion. In pH-ZRCCC separation, a solvent system is selected as ethyl acetate-acetonitrile-water (4: 1: 5, v / v / v), trifluoroacetic acid (10mM) is added into an upper phase as a stationary phase, and ammonia water (10mM) is added into a lower phase as a mobile phase. Caffeic acid, hydroxycinnamic acid and a mixture A are separated from the dandelion ethyl acetate crude extract; hSCCC is further utilized, and a solvent system is selected as petroleum ether-ethyl acetate-methanol-water (1: 4: 1: 4, v / v / v / v). 1-O-caffeoylglycerol, 3, 4-dihydroxyphenylacetic acid, protocatechuic acid and p-hydroxyphenylacetic acid are obtained through separation from 400 mg of the mixture A. The method has the advantages of large preparation amount and good reproducibility, and is suitable for separation and purification of dandelion polyphenol compounds.

Description

technical field [0001] The invention belongs to the technical field of chemical analysis, and in particular relates to a high-efficiency separation method for polyphenol components in dandelion. Background technique [0002] Dandelion (Taraxacum mongolicum Hand.-Mazz.) is a perennial herb of Asteraceae, which has the functions of clearing away heat and detoxifying, eliminating carbuncle and dissipating stagnation. Studies have shown that dandelion polyphenols have pharmacological activities such as anti-oxidation, anti-cancer, antibacterial, anti-inflammatory, and hypoglycemic. In order to further study the pharmacological activity of dandelion polyphenols, it is necessary to separate and prepare more monomeric compounds. Dandelion polyphenols have complex components and are difficult to separate and prepare. Currently, the commonly used separation and preparation methods are column chromatography, which has low sample preparation efficiency and takes a long time. Counterc...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/42G01N30/34G01N30/36G01N30/32G01N30/74
Inventor 相宏杰刘炬希达悦·侯赛因
Owner QIANFOSHAN HOSPITAL OF SHANDONG
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