Wheat stripe rust resistance related metabolite as well as synthesis related gene and application thereof

A technology related to wheat stripe rust and resistance is applied in the field of plant genetic engineering, which can solve the problems of loss of stripe rust resistance, population destruction, and wheat yield reduction of main wheat varieties, and achieve green prevention and control of wheat stripe rust and corn rust. The effect of germination and ability improvement

Pending Publication Date: 2022-03-15
SOUTHWEST UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, due to the influence of climate, variety and other reasons, wheat is always threatened by various diseases, resulting in reduced production, and wheat stripe rust is one of the very important diseases
[0003] Wheat stripe rust is a disease caused by Puccinia striiformis f.sp.tritici (Pst), due to its rapid pathogenic variation and frequent variation, outbreaks, epidemics, and long-term , recurrence and other characteristics, it is easy to cause the frequent "loss" of resistance to stripe rust in the main wheat varieties, leading to the outbreak of wheat stripe rust, which seriously threatens the safe production of wheat worldwide
[0004] At present, the prevention and control of this disease is mainly chemical control, but the long-term and excessive use of chemical agents has caused the destruction of beneficial microbial populations in the soil, and environmental pollution problems such as excessive heavy metals

Method used

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  • Wheat stripe rust resistance related metabolite as well as synthesis related gene and application thereof
  • Wheat stripe rust resistance related metabolite as well as synthesis related gene and application thereof
  • Wheat stripe rust resistance related metabolite as well as synthesis related gene and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Example 1 Analysis of metabolites after stripe rust fungus CYR23 infects wheat

[0028] (1) Material handling

[0029] Disinfect the wheat seeds of Suwon11 (Suwon11) with 75% alcohol, wash them with ultrapure water 4-5 times, soak the seeds to accelerate germination, sow them in a nutrient bowl, and cultivate them in an environment of 12°C±2°C until the wheat seedlings grow to the 1-leaf stage At the same time, the physiological race CYR23 of stripe rust was smeared on the leaves, kept moist for 24 hours in the dark, and the normal growing wheat was used as the control CK. The samples were collected at 12h, 24h, and 48h after inoculation.

[0030] (2) Detection of metabolites by ultra-high performance liquid chromatography and tandem mass spectrometry

[0031]The metabolites of the materials obtained in step (1) were detected by ultra-high performance liquid chromatography and tandem mass spectrometry. The specific operation method is: S1, Standards and reagents, all...

Embodiment 2

[0033] Anti-stripe rust experiment after embodiment 2HGA foliar spraying of wheat and corn

[0034] (1) Material cultivation

[0035] Disinfect the wheat seeds of Shuiyuan 11 with 75% alcohol, wash them with ultrapure water 4-5 times, soak the seeds to accelerate germination, sow them in a nutrient bowl, and cultivate them in a greenhouse at 12°C±2°C, with 16 hours of light and 8 hours of darkness. After disinfecting the corn seeds with 75% alcohol, wash them with ultrapure water 4-5 times, soak the seeds to accelerate germination, sow them in a nutrient bowl, and cultivate them in a greenhouse at 25°C±2°C with 16 hours of light and 8 hours of darkness.

[0036] (2) Material handling

[0037] Spray HGA solutions with concentrations of 2.5mmol, 5mmol, and 10mmol on the leaves of wheat respectively. Each treatment is repeated 3 times. The control group CK is not sprayed. After 12 days, the degree of disease on the wheat leaves was observed.

[0038] Spray HGA solutions with a...

Embodiment 3

[0040] Embodiment 3 HGA is to the influence experiment of wheat stripe rust spore and common rust spore germination rate of corn

[0041] Prepare HGA solutions with concentrations of 1mmol / L, 2mmol / L, 5mmol / L, and 10mmol / L respectively. Take a clean glass slide, add a drop of the above-mentioned HGA solution of different concentrations, and add a drop of distilled water to the control group CK, pick a small amount of stripe rust spores with a dissecting needle and place them in the above-mentioned solutions of different concentrations and distilled water respectively. Adjust the spore concentration under the microscope, about 40-60 spores / field of vision. Place the slides in a petri dish lined with absorbent paper, and then culture them in an incubator at 12°C±2°C. After 24 hours, it was taken out for observation, and each treatment was repeated 3 times. The corn rust spore inhibition experiment method was the same as above, and the culture temperature was 20°C±2°C.

[0042]...

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Abstract

The invention belongs to the technical field of plant genetic engineering, and particularly relates to a wheat stripe rust resistance related metabolite and a synthesis related gene and application thereof. In the combined analysis of metabolome and transcriptome of wheat infected by stripe rust, 2, 5-dihydroxyphenylacetic acid (HGA) and a synthetic related gene TaHPD thereof are induced to be up-regulated, and the resistance of wheat and corn to rust diseases can be improved and the germination of uredospores of the stripe rust can be inhibited by externally spraying the HGA. Gene silencing and TaHPD overexpression also prove that the change of the HGA content affects the disease resistance of plants. The compound HGA provided by the invention can be processed into a prevention and control agent to realize green prevention and control of crop diseases.

Description

technical field [0001] The invention belongs to the technical field of plant genetic engineering, and in particular relates to a natural compound HGA screened out in the non-affinity interaction between wheat and stripe rust, its synthesis-related genes and its application. Background technique [0002] Wheat is a food crop with wide planting range, high yield and long planting history in the world. Foods such as bread, noodles, and steamed buns processed from wheat have also been loved by everyone and occupied the consumer market. According to statistics, China's wheat planting area increased from 24.0716 million hectares in 2014 to 24.5104 million hectares in 2017, and its output increased from 126.2152 million tons in 2014 to 134.3406 million tons in 2017, showing an increasing trend year by year. It can be seen that China's demand for wheat continues to increase. According to this growth rate, it is estimated that by 2050, the demand will increase by 60%. However, due ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/53C12N9/02C12N15/84A01H5/00A01H6/82A01N63/50A01P3/00
CPCC12N9/0069C12N15/8218C12N15/8282C12N15/8205A01N63/50C12Y113/11027
Inventor 杨宇衡刘赛斐马子慧肖牧野宿嘉轩田斌年方安菲余洋毕朝位
Owner SOUTHWEST UNIV
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