The invention relates to a special strawberry composite biological fertilizer as well as a preparation method and application thereof. The special strawberry composite biological fertilizer comprisesthe following components: EDTA-Ca (Ethylene Diamine Tetraacetic Acid), magnesium sulfate heptahydrate, EDTA-Mg, heptahydrate, EDTA-Zn, manganese sulfate monohydrate, EDTA-Mn, boric acid, EDTA-Fe, EDTA-Cu, soybean molasses, polyglutamic acid fermentation broth, chitosan oligosaccharide, algin, compound amino acid, humic acid, bacillus thuringiensis fermentation broth, bacillus megatherium fermentation broth, bacillus mucilaginosus fermentation broth and streptomyces jingyangensis fermentation broth. Through bacterium activation, seed liquid preparation, fermentation culture and compounding, thecomposite biological fertilizer is prepared, the composite biological fertilizer is applied to strawberry planting, and a diluted composite biological fertilizer liquid is sprayed at different growthstages of strawberry respectively. Due to synergetic effects of substances such as inorganic salts, organic matters and microorganisms, long lasting nutrient substances are provided to strawberry crops, absorption of nutrients in soil by plants is promoted, the fertilizer amount is reduced, and the chemical fertilizer cost is reduced.