Experiment method for studying effect of tenuigenin on synaptic transmission of rat hippocampal neuron
A hippocampal neuron and polygala saponin technology, which is applied in measuring devices, material analysis through electromagnetic means, instruments, etc., can solve the problem that neurological drug experiments cannot achieve the desired effect, and the pharmacological mechanism of neurological drugs cannot be clearly explored. It can meet the needs of neuropharmacological research and other issues
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[0096] The technical scheme adopted in the present invention is a test method for the effect of polygala saponins on the synaptic transmission of rat hippocampal neurons, and the medicines and reagents used in the test method:
[0097] Artificial cerebrospinal fluid, namely ACSF, its components are: NaCl 124 mmol / L, KCl 2.5 mmol / L, NaHCO 3 26 mmol / L, NaH 2 PO 4 1.25 mmol / L, CaCl 2 2. 0 mmol / L, MgSO 4 1.3 mmol / L, D-glucose 10 mmol / L, pH 7.4;
[0098] The electrode inner solution for recording spontaneous postsynaptic current, its composition is: KCl 140 mmol / L, NaCl 6 mmol / L, HEPES 10 mmol / L, EGTA 10 mmol / L, Mg-ATP 2 mmol / L, NaGTP is 0.1 mmol / L, pH value is 7.2;
[0099] Electrode inner solution for recording spontaneous excitatory postsynaptic current, its components are: K gluconate 140 mmol / L, NaCl 6 mmol / L, HEPES 10 mmol / L, EGTA 0.2 mmol / L, Mg-ATP 2 mmol / L, Na-GTP 0.1 mmol / L, pH 7.2;
[0100] The electrode inner solution for recording spontaneous inhibitory pos...
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