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Cordyceps sinensis epiphyte and artificial cultivation method thereof

A technology of artificial cultivation and Cordyceps, applied in the field of microorganisms, can solve the problems of lack of resources, low natural yield, and destruction of ecological balance in plateau areas.

Inactive Publication Date: 2008-06-11
华富生物科技(上海)有限公司 +1
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  • Abstract
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AI Technical Summary

Problems solved by technology

For a long time, domestic medicinal Cordyceps has been collected from the wild. Due to the strict parasitism of Cordyceps sinensis and the particularity of the ecological environment requirements, the natural yield is very low. In addition, the resources are very scarce due to artificial blind excavation, and it has seriously damaged the plateau area. ecological balance

Method used

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Embodiment Construction

[0021] 1. Preparation of solid medium: Take potatoes that have not germinated and turned green, wash and peel them, weigh 200g, and cut into small pieces about 1 cm square. Put the cut potato cubes into about 1000-1200mL of water, boil and keep for 30 minutes with warm fire. After finishing, filter with gauze, obtain the filtrate as potato juice, make up the filtrate to 1000mL. At the same time, add 10 g of brown sugar, 10 g of glucose, and 20 g of PDA (potato starch: glucose: agar = 4: 20: 15), stir them evenly, pour them into test tubes, and sterilize them at 121 ° C for 60 minutes. It can be used for replanting after cooling and solidification, and the dosage can be adjusted proportionally when a large amount of cultivation is required.

[0022] 2. Preparation of liquid medium: Take potatoes that have not germinated and turned green, wash and peel them, weigh 200g, and cut them into small pieces about 1 cm square. Put the cut potato cubes into about 1000-1200mL of water, ...

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Abstract

The invention relates to worm grass fungus and the artificial culture method thereof, which belongs to the micro organism technology field. The worm grass fungus disclosed by the invention is high essence worm grass fungus Paecilomyces tenuipes(Peck)Samson=P. tenuipes, the artificial culture method comprises the strain activation, the mycelium culturing through liquid culture method and the fruiting body culturing with silkworm pupa. The invention adopts the main steps that the silkworm pupa is adopted as the host plant of the artificial cultured worm grass, and the fruiting body culturing with silkworm pupa is divided into two steps as follows: earlier stage (o to 5-15 days) and later stage (5 to 15 days until collection), lucifuge culturing is adopted in the earlier stage, illuminantion, abundant oxygen supply and more than 75 percent of ambient humidity are ensured in the later stage, and compared with the earlier stage, the ambient temperature of the later stage is obviously reduced; the invention can meet the scale production of the artificial worm grass.

Description

technical field [0001] The invention belongs to the technical field of microbes, and in particular relates to a high-gensu Cordyceps strain rich in active substances and an artificial cultivation method thereof. Background technique [0002] Cordyceps sinensis is a unique and precious medicinal material in my country. It is listed as the three important treasures together with ginseng and velvet antler. Traditional Chinese medicine believes that Cordyceps sinensis has the functions of tonifying deficiency, replenishing essence, protecting lungs, and benefiting kidneys. In recent years, medical research has found that it also has sedative, Anti-hypoxia, anti-inflammation, antihypertensive, improving myocardial ischemia and improving human immunity and other functions. For a long time, domestic medicinal Cordyceps has been collected from the wild. Due to the strict parasitism of Cordyceps sinensis and the particularity of the ecological environment requirements, the natural yie...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14
Inventor 巢正国钟江马红岩
Owner 华富生物科技(上海)有限公司
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