370results about How to "Improve seedling quality" patented technology

The invention discloses a high-quality and high-output planting method for hot pepper. The method is characterized in that the seeds are dried and soaked through a clean plastic basin 2 to 5 days before sowing; the seed is disinfected before sowing; the seed which is expanded in water is transferred to a proper temperature to prompt the seed germination; the germination prompting temperature is controlled to be 20 to 30 DEG C, and the relative humidity is controlled to be about 90%; when seedlings grow from the seed coat and the roots of the seedlings are as long as the seed, the seed can be sowed; the culture medium soil for culture of seedling comprises vermiculite, wormcast, decomposed cow dung, straw bran, saw dust and sieved soil; the land is leveled, the fertilizer is applied, then the seedlings are transplanted and planted to the field. According to the method, the components of the culture medium soil are changed; in addition, the grass carbon is decreased; lots of wormcast, decomposed cow dung, straw bran and other unused wastes in the agricultural planting and breeding industry are effectively utilized to form a composite culture medium which improves the seedling culture quality and lowers down the cost of seedling culture; compared with the original planting method, the planting method has the advantages that the seeds are scientifically treated, the management after field planting is scientific, and therefore, the output of the hot pepper is ensured and reaches 5,000g / mu, and the output is increased by about 20 to 30%.

The invention discloses a liquid film prepared from crop straw fermentation liquid and an application method thereof. The liquid film comprises the following components in parts by weight: 82-99 parts of raw materials, 11-14 parts of film forming agent, 2-3 parts of plasticizer, 1.3-2.5 parts of crosslinking agent, 0.02-0.05 part of initiator, 0.2-0.3 part of pH regulator and 0.1-0.3 part of additional component, wherein the raw materials include 68-79 parts of crop straw fermentation waste liquid or water, 7-10 parts of bentonite, 2-3 parts of modified starch and 5-7 parts of potassium humate; and the additional component is one or more of crofton weed extraction liquid, potassium nitrate, trace elements and composite microbial agent spore powder. The liquid film disclosed by the invention has the advantages of simple proportioning, simple structure, convenience in use, low cost, low labor intensity and degradability; and after degradation, the liquid film can improve the soil texture and aggregate structure so as to improve the fertilizer efficiency.

The invention discloses a two-finger four-pin automatic picking and placing device for plug seedling transplanting. The device comprises a mechanical cantilever sleeve 1, a mechanical finger frame 2, rubber fingers 4, a support rod 5 and a mechanical finger spacing block 9 which are fixedly connected in sequence along the direction of the same center line, wherein each mechanical finger comprises a mechanical finger joint 3, a micro-cylinder 6, a mechanical finger sleeve 7, a clamping pin spacing block 11 with a longitudinal spacing groove and a clamping pin 14 which can slide in the longitudinal spacing groove and is fixedly connected with a piston rod of the micro-cylinder 6; the upper ends of the mechanical finger joints 3 are symmetrically hinged on two sides of the mechanical finger frame 2; the mechanical finger sleeve 7 passes through the chute of the mechanical finger spacing block 9, and can move in the chute along the chute direction; the two mechanical fingers and the support rod are pre-tightened together through a tightening spring 8; and the tightening spring 8 is positioned on one side of the mechanical finger spacing block 9, far from the tail end of the mechanical finger, and is close to the mechanical finger sleeve 7. The device is low in cost and hardly damages seedlings, and can be used for automatic transplanting of plug seedlings with different specifications.

The invention discloses a special fertilizer with medical fertilizer slowly released and water conserved for seedlings growth which is characterized in that the fertilizer mainly consists of the following ingredients according to weight propotion: 5-10 percent of super-absorbent resin, 8-20 percent of nitrogen (N), 5-12 percent of phosphorus pentoxide (P2O5), 3-7 percent of potassium oxide (K2O), 1-3 percent of zinc (Zn), 1-3 percent of ferrite (Fe), 1-5 percent of ingredients of boron, magnesium, molybdenum, manganese and copper, 0.2-2 percent of pesticide, 0.1-2 percent of fungicide, 0.001-0.01 percent of root growth promoting agent and 0.001-0.01 percent of dwarfing agent and the rest is adsorbent. The special fertilizer with medical fertilizer slowly released and water conserved for seedlings growth of the invention has functions of good water holding and supplying, slow releasing of medical fertilizer, seedlings and roots strengthening, elements applying and growth promotion, diseases prevention, insect disinfesting and production increasing, and is characterized by less dosage, low cost and convenient application, etc., and can be applied to rice seedlings dry raising, seedling raising on plastic trays and upland crops raising.

A combined equipment for artificially culturing plants is composed of a number of hexagonal transparent culture containers which are combined in cellular mode, the overflow unit of nutritive liquid, nano lamp, humidity controller, CO2 source, speed-adjustable motor, blower and pipelines. Its advantages are low pollution, high quality of seedlings and short culture period.

The invention discloses a flue-cured tobacco cultivation method fitting in with the light environment of the Yunnan tobacco-growing area and belongs to the technical field of tobacco plant cultivation. The flue-cured tobacco cultivation method comprises the following steps of float breeding, soil preparation and ridging, transplanting under film, management in film covering period and field management. The seeding time is from mid December to mid February, the float disc floating seedling method is adopted, and seeding hardening lasts for 8-14 days before transplanting; ridging is conducted after the soil is pulverized and moisture is stored, and inorganic fertilizer and cake fertilizer which is thoroughly decomposed are mixed to be applied to the field; the transplanting period is from mid March to mid April, the density of tobacco plants is 1000-12000 plant / mu, each tobacco plant is irrigated with 1.5-2.5kg root water after transplanting, and colored mulching film is covered; top dressing is conducted twice after transplanting, and seeding leading is conducted 15-25 days after the top of each tobacco seeding ejects the film; the mulching film is removed when the tobacco plants enter the mid of the vigorous growth period, earthing up, weeding and watering are conducted in time, and topping, leaf reserving and bifurcation removing are conducted. The flue-cured tobacco cultivation method can fitting in with the light environment of the Yunnan tobacco-growing area, the local light source can be utilized fully and reasonably, the quality of tobacco leaves can be improved, and the aroma style of the fen-flavor tobacco leaves is enhanced.

An automatic sower for culturing seedlings in plastic trays features that the plastic trays are put on the streamline mechanism composed of conveying belts, and the basic soil applicator, seed sowing unit, covering soil applicator and soil pressing roller are sequentially arranged on the machine frame above said plastic trays.

The invention discloses an automatic seedling tray seeding assembly line. The seeding assembly line is sequentially fixed with a seedling tray upper tray device, a substrate laying device, a precision roller sowing device, a spraying device, a substrate covering device, and a seedling tray stacking device. A seedling raising tray is placed above the seedling raising tray upper plate device, and the seedling raising tray is pushed to the sowing line along the conveyor belt. The working efficiency of the assembly line of the present invention is 10 times that of the traditional artificial seedling raising mode, reduces the labor cost by two-thirds, and can significantly improve the quality of seedling raising. The operation is simpler, the work efficiency and quality are higher, the commonality of parts is better, the reliability and safety are higher, and the market prospect is very broad.

The invention discloses a botanical seedling culturing machine. A control system and a water pump, an air compressor, an air conditioner and a pre-heater which are electrically connected with the control system are arranged in a primary unit segment and an external ventilator and an internal gas circulator which are electrically connected with the control system arranged in the primary unit segment are arranged in an auxiliary unit segment, at least one group of culturing chests of drawers (5) and sliding rails (18) for drawer; each group of culturing chests of drawers (5) are provided with operation shelves (13), working inter-layers (14) and movable meshes (16), and movable working discs (15); ventilation and fertilizer feed holes are arranged at the bottom of the movable working disc (15), a light strip module (28), a spraying pipeline and a nozzle (29), a high-pressure air pipe (30), and the reflux tank (32) of an irrigation tank (31) are arranged in the rippled grooves downwards of a plurality of notches on the working inter-layer (14). The invention is a plastic potted tray botanical seedling culturing machine without foam, polystyrene, and polypropylene capable of high-quality industrialized, intensified and entire-year seedling culturing seedling production of scale.

A fast propagation method of tissue culture of Dendrobium primulinum Lindl belongs to the technical field of plant fast propagation. The method is characterized by comprising the steps: 1) a young stem of the Dendrobium primulinum Lindl with a length of 3 to 5cm is chosen as an explant; 2) the treating of the explant; 3) inoculation and culture for 10 to15 days in an aseptic inoculation chamber; 4) a survived stem tip is transferred to a inducing medium for inducing an adventitious bud or a protocorm which, after 30 to 40 days, is transferred to a reproducing medium for reproducing of 2 to 3 months; 5) successive transfer and rooting culture; 6) a single plant is transplanted in a 128 plug-tray. The fast propagation method of tissue culture of the Dendrobium primulinum Lindl is not affected by seasons. Due to the easy controlled propagation environment, the Dendrobium primulinum Lindl adapts to various places of China. Therefore, the problems of hard-breeding, easy degeneration, quick variation and irregular seedling quality of the Dendrobium primulinum Lindl are resolved. Seedlings of the Dendrobium primulinum Lindl with good and uniform quality are produced and have wide market prospect.

The invention relates to a soilless seedling compound substrate formula for solanaceous fruit vegetables, namely a soilless seedling compound substrate formula by replacing peat with mushroom residue parts of cottonseed hulls. The waste cottonseed hulls after edible mushroom planting are subjected to pretreatment such as retting fermentation, air drying, smashing, sieving and the like, and are fully and uniformly mixed with the peat and vermiculite according to a ratio of the cottonseed hull to the peat to the vermiculite (with the diameter of about 1 mm) of 1:1:1 (v / v) to form the compound substrate special for raising seedlings. Compared with the conventional seedling substrate with a ratio of the peat to the vermiculite of 2:1 (v / v), the substrate is suitable for the soilless seedling for the solanaceous fruit vegetables, has the advantages of resource saving, cost reduction, safety, environmental protection, no pollution, effective improvement of seedling quality and efficiency and the like, and is favorable for improving the cost-saving, high-efficiency and sustainable development of industrialized seedling raising.

The invention provides an automatic potted seedling transplanter and a control method thereof. The automatic potted seedling transplanter comprises a chassis bracket, planting mechanism, seedling separating mechanisms, seedling guiding devices, a power mechanism, a moving box mechanism and a seedling picking mechanism, the moving box mechanism mounted on the chassis bracket, the seedling picking mechanism is mounted on the moving box mechanism, two sets of the seedling separating mechanisms are symmetrically arranged at two sides of the moving box mechanism through the chassis bracket, the seedling guiding devices are arranged above the seedling separating mechanisms, and the planting mechanisms are arranged below the seedling separating mechanisms. The automatic potted seedling transplanter can realize non-interval whole row seedling picking of pots with various specifications and seedling dropping under the synergistic effects of all the mechanisms and a control system, so the efficiency and the success rate of seedling picking and dropping are improved; the pots are only longitudinally conveyed and moved, so the pot conveying efficiency is improved; pins of seedling claws have acarding effect on potted seedlings before being inserted into the pots, so the seedling picking quality is improved; and the seedling guiding devices receive the seedlings in a stationary state, andshorten a free-fall motion distance, so seedling dropping is accurate.

The invention discloses a plant tissue culture rooting and seedling exercising integrated cultivating device and a cultivating method thereof. The cultivating device comprises a plant cultivation system, an illumination system, a ventilation temperature-control system, a fluid supplying system, a CO2 supplying system and an environment control system. The plant cultivation system comprises a three-dimensional multi-layer cultivation frame and cultivation containers placed on the cultivation frame, each layer of multiple layers of the cultivation frame is provided with one cultivation container, and lifting seedling disc supports are arranged at the bottoms of the containers and used for suspending cultivation plants. The illumination system comprises lamp boxes, LED lamp tubes and fans, and the LED lamp tubes are arranged inside the lamp boxes and connected with the fans to be installed above the cultivation containers in an integrated mode without communication with the cultivation containers. On the premise of guaranteeing that space and time are saved, the cost is low, and the utilization rate of resources is high, the utilization rate of the resources and labor productivity are increased, the cultivation period is shortened, and the production cost is lowered.

The invention discloses a rapid cutting propagation method of crape myrtle single-plant germplasm. On the basis that crape myrtle single plants with the good characters of the natural tree type, flowering habit, flower color and flower diameter size and the like are surveyed and screened out, incising and sprouting promotion is performed on optimized single-plant rootstock portions or purging and sprouting promotion is performed on the surface soil layer lateral roots to form young root sucker coppice shoots at the developmental stage, the coppice shoots are used for ear making and cutting propagation, cutting seedlings are used for building a cutting orchard, cutting orchard building and management are performed by routine, scion wood is completely collected and processed on the base of the trunk of the stock plant to promote sprouting and cultivation of coppice shoots, the standard quality of ear cutting and making is strictly regulated, the cutting seedbed matrix arrangement is simplified, rooting accelerator arrangement and using method are optimized, limitation of the cutting season is broken through, rapid long-term large-scale cutting seedling is achieved, the cutting survival rate and nursery stock quality are improved, and the cutting seedling cost is lowered.

The invention relates to a high-yield cultivation method for honeysuckles in low mountains and hills. The method includes the key technical steps such as variety selection, raising of seedlings by cutting, digging of planting holes, base fertilizer application and planting, covering of mulching films, pruning, integrated control of plant diseases and insect pests and topdressing. The method is characterized in that growth and development of plants of the honeysuckles are obviously promoted, the time limit ahead of the high-yield period is shortened, the yield of the honeysuckles is improved, and the quality of the honeysuckles is guaranteed. Compared with an ordinary planting method, the high-yield cultivation method enables the yield in the first year after transplant to be promoted by 56.7%, the yield in the second year after the transplant is promoted by 87.3%, and the yield in the third year after the transplant is promoted by 116.6%. However, no obvious changes happen on the content of chlorogenic acid of the honeysuckles and contents of galuteolin of the honeysuckles, the content of the chlorogenic acid of the honeysuckles and the content of the galuteolin of the honeysuckles all meet the standard provisions of <pharmacopoeia of the people's republic of China> (2010 version), and social benefits, economic benefits and ecological benefits are remarkable.

The invention discloses a seedling cultivation hole tray which comprises a growth tray used for containing a seedling cultivation substrate and a covering tray located above the growth tray to correspondingly match with the growth tray and used for sowing, wherein the growth tray is composed of an array which are formed by multiple growth holes; the covering tray is composed of an array which is formed by multiple covering hole grooves; the growth holes and the covering hole grooves correspondingly match with each other in the aspects of number and position. The seedling cultivation hole tray simplifies multiple procedures of seedling cultivation and sowing links of the hole tray, realizes streamlined sowing technology and efficient operation, reduces sowing employment rate and also realizes digital quantitative control and standard management of the sowing height and the covering depth, and further improves seedling cultivation quality. In addition, the invention also provides a method for seedling cultivation and sowing by utilizing the seedling cultivation hole tray, which can realize efficient sowing, simple operation and strong operability.

The invention discloses a rapid breeding method of lamina seedlings of tinospora capillipes gagnep. The method comprises the steps: (1) scratching the back sides of tender laminas of sterile test tube seedlings of tinospora capillipes gagnep to be well-shaped by using a scalpel and putting into an MS propagation medium for culturing; performing induction formation on callus and classifying to obtain multiple shoots; (2) putting the multiple shoots into an MS strong seedling medium for culturing strong seedlings to obtain strong plants; and (3) putting the strong plants into a 1 / 2 MS rooting medium to obtain complete root seedlings. The proliferation coefficient of the multiple shoots of the tinospora capillipes gagnep obtained by using the culturing method reaches 20-30 times, the rooting rate of the obtained tissue culture seedlings is more than 95 percent, the survival rate of seedbed transplanting is more than 90 percent, and the scale seedling breeding problem of the tinospora capillipes gagnep is effectively solved.

The invention relates to a seedling culture method capable of improving emergence rate of gentiana crassicaulis, belonging to the technical field of medicinal plant cultivation. The seedling culture method comprises the following steps: seedlings are cultured at altitude of 2200-2600m and transplanted at altitude of 2200-3300m; flat ground with deep and fertile soil layers or gentle slope with deep and fertile soil layers is used for culturing the seedlings; then the whole ground is ridged and covered with a film; before seeding, the seeds are soaked with clean water at 20 DEG C for 24 hours or with 50ppm gibberellin for 2-8 hours; the land plot with rust diseases or root rot diseases is subjected to seed disinfection; after soaking or disinfection, wet sands are mixed with the seeds to be processed for 20-30 days; after the seeds are processed, the film is uncovered; grass on the ground is removed; and the ridged surface is leveled; the seeds are sown at low density; after sowing, the ground is covered with mixed soil and pine leaves; 7-8 months later, fertilizer is applied to the ground for 2-3 times, and Bordeaux mixture is sprayed. The seedling culture method has the advantages that the emergence rate of the gentiana crassicaulis can be increased to 65-94%; diseases at a seedling stage can be relieved; and seedling quality and strong seedling output rate can be improved.

A subprostrate sophora root tissue culture seedling industrialization production method comprises the following steps: subprostrate sophora root tissue culture seedling breeding and rejuvenation, rooting domestication and transplantation. By adopting the method, a great amount of excellent, suitably mountain-cultured subprostrate sophora root tissue culture seedling with more than 98% transplantation survival rate can be provided in short time, so as to meet production demand.

The invention relates to a method for performing in-vitro culturing and rapid propagating on bergenia crassifolia. The method comprises the following steps of: selecting a fresh rhizome with terminal buds as a sterile exophyte, and inoculating the sterile exophyte to a primary culture medium to carry out primary culture; transferring a primarily-cultured aseptic seedling to a proliferation culture medium, wherein the proliferation culture medium comprises 0.5-3.5 mg.L<-1> of MS (Murashige and Skoog)+BA (Butyl Acylate), 0.2-1.5 mg.L<-1> of NAA (Naphtyl Acetic Acid), 0.5-5.0 mg.L<-1> of VC (Vitamin C), 25-35g.L<-1> of sucrose and 4-84.L <-1> of agar; inducing adventitious buds; slitting the induced cluster buds and transferring the slit cluster buds to a strong seedling culture medium for culturing the strong seedling; slitting test-tube seedling and transferring the slit test-tube seedling to a rooting medium, wherein the rooting medium comprises 1.5-4.5 mg.L<-1> of 1 / 2MS+NAA, 0.5-2.5 mg.L<-1> of IBA (Indole Butyric Acid), 0.5-5.0 mg.L<-1> of VC, 15-25.gL<-1> of sucrose and 4-8g.L<-1> of agar; cleaning and transferring the rooted test-tube seedling to a substrate for planting. The method can be used for increasing a yearly propagation coefficient to 1:600000 and reducing the cost to 2-3 yuan per plant, and is free of season limitation and easy in environment control, so that variant plants are reduced, the quality of the seedling is high, and the consistency is good.

The invention provides a device and method for efficiently collecting sargassum fertilized eggs. The device comprises a seawater filter device, a stirring device and a collector, wherein the stirring device comprises a stirring tank and a stirrer; the stirrer is arranged below the stirring tank; a stirring blade of the stirrer is arranged in the stirring tank; a stirring tank filter screen is arranged in the stirring tank; a colleting opening valve is arranged at the bottom or on the side of the bottom of the stirring tank; the collector is arranged below the colleting opening valve; and the collector is provided with at least two collector filter screens inside. By adopting the device and the method, the problems of low degree of mechanization, high labor intensity, intensive labor time,small seedling culture scale, low utilization ratio of seed resources and the like in the seedling collection process in the prior art are solved.

The invention relates to a tissue culture rapid-propagation method of beaucarnea recurvata. The tissue culture rapid-propagation method comprises the following steps: (1) placing a disinfected beaucarnea recurvata seed as an explant into an MS germination culture medium for germination induction to obtain a sterile test-tube seedling; (2) placing the test-tube seedling into an MS cluster bud culture medium for propagation culture to obtain cluster buds; (3) placing the cluster buds into an MS strong seedling culture medium for culture to obtain a strong plant; (4) placing the strong plant into a 1 / 2MS rooting culture medium for culture to obtain a rooting seedling; (5) carrying out seedling adaptation on the rooting seedling, and transplanting to a sand bed to grow for one month, and then transplanting to a field. According to the tissue culture rapid-propagation method, the multiplication coefficient of the obtained cluster buds is 20-30 times, the rooting rate of an obtained tissue culture seedling is more than 95%, and the survival rate of a transplanting seedbed is more than 90%, so that the problem of industrialized seedling culture of the beaucarnea recurvata is effectively solved.

A radix gentianae dahuricae cultivation method comprises the following steps of land selection and preparation, sowing and seedling raising, transplanting, field management, pest control and prevention, harvesting and processing.Introduction and domestication are performed on radix gentianae dahuricae wild resources, large-scale artificial cultivation is achieved, normalized and standardized production technology is promoted, rapid growth needed by radix gentianae dahuricae is met, and resource sustainable utilization of the radix gentianae dahuricae is maintained.

The invention discloses a method for efficiently propagating dendrobium by using roots and a culture medium of the dendrobium. The method in the invention comprises the following steps of: selecting a root section of the dendrobium, and incubating and inducing the root section to obtain an embryonic callus, wherein the embryonic callus can be greatly multiplied through subculture; transferring the embryonic callus into a protocorm induction culture medium for induction and multiplication to obtain protocorm and bud seedlings; and transferring the protocorm and the bud seedlings into an adult seedling culture medium and culturing a dendrobium rooting plant. According to the method, MS is used as a basic culture medium; 6-BA and PIC are required to be added into the culture medium for the embryonic callus induction or subculture; CPPU and NAA are required to be added into the culture medium for the protocorm induction. The method for culturing regenerated plants by using the dendrobium roots provided by the invention is high in propagation coefficient, high in planting percentage and low in cost; a seedling growing process, a seedling robusting process and a rooting progress of the protocorm can be further finished; the plants are robust, and the roots are robust.

The invention discloses a method for producing a solanaceous vegetable seedling growing substrate by utilizing a salvaged material of cassava processing. Cassava peels and starch residues are used as main raw materials, chicken manure and seed tung tree bran or chicken manure and shell powder are used as auxiliary materials, and the excellent solanaceous vegetable seedling growing substrate is produced through fermentation. The substrate is light and loose, has the advantages of good permeability, good water holding and fertilizer retaining abilities, and balanced, scientific and reasonable nutrient supply, can meet the balance demand of solanaceous vegetable crop seedlings on nutrients during a whole growth period, and effectively improves the survival rate, the stress resistance and the growing seedling quality of the solanaceous vegetable growing seedlings. The method realizes harmless treatment and resource utilization of the salvaged material of cassava processing, avoids great waste of resources and serious pollution of the environment, provides a new way for comprehensive utilization of the agricultural salvaged material, reduces the substrate production cost, reduces excessive exploitation of peat resources, protects the natural environment, and has wide application value.

The invention discloses a method for accelerating germination of paris polyphylla seeds. The method comprises the following steps: step one, taking paris polyphylla seeds and soaking the paris polyphylla seeds in a gibberellin solution in mass-volume concentration of 0.1-0.5% at the temperature of 20-30 DEG C; step two, carrying out ultrasonic treatment on the paris polyphylla seeds soaked by gibberellins; step three, carrying out dehydration treatment on the paris polyphylla seeds subjected to ultrasonic treatment; and step four, carrying out fluctuating-temperature stratification treatment on the paris polyphylla seeds subjected to the dehydration treatment: firstly carrying out stratification on the paris polyphylla seeds for a period of time under the constant-temperature condition at the temperature of 5 DEG C, then carrying out stratification on the paris polyphylla seeds for a period of time under the constant-temperature condition at the temperature of 25 DEG C, and finally carrying out stratification of the paris polyphylla seeds for a period of time under the constant-temperature condition at the temperature of 5 DEG C. According to the method, the germination rate of the paris polyphylla seeds after one month is 24%; the germination rate of the paris polyphylla seeds after two months is 86%; the uniformity of the emerged seedlings is high; the number of normal seedlings accounts for 95% of the number of germinated seeds; the quality of the paris polyphylla seedlings is greatly improved.

The invention provides a soilless culture seedling substrate for angelica and a preparation method thereof, which relate to the technical field of soilless culture. The seedling substrate is prepared with the following materials in parts by weight: 200 to 300 parts of traditional Chinese medicine dregs, 150 to 200 parts of bagasse, 100 to 130 parts of chaff, 60 to 90 parts of shaddock peel powder, 60 to 90 parts of mushroom residue, 30 to 50 parts of sucrose, 20 to 30 parts of ammonium sulfate, 150 to 200 parts of cow dung, 30 to 50 parts of beer yeast, 30 to 50 parts of aspergillus niger and 200 to 300 parts of vermiculite. When the substrate is used for culturing angelica seedlings, the seedlings are uniform in size, and have high resistance and high quality, and the bolting rate is low after planting.

The invention discloses a culture medium to promote growth of Pericallis hybrida. The culture medium is composed of a sprouting medium and a seedling growth medium; the sprouting medium is composed ofpearlite, fermented decomposed cow dung and humic acid solution; the seedling growth medium is composed of fermented decomposed cow dung, straws, biochar, activated humic acid, peat soil and vermiculite, wherein the pearlite, fermented decomposed cow dung and humic acid solution in the sprouting medium have a volume ratio of 3:1:0.1; the content of humic acid in the humic acid solution is 6%; thefermented decomposed cow dung, straws, biochar, activated humic acid, peat soil and vermiculite in the seedling growth medium have a volume ratio of 2:(8-10):1:1:4:2. The invention also discloses a culture method to promote growth of Pericallis hybrida. Sprouting, rooting and quick growth of Pericallis hybrida can be promoted herein through a simple seeding method and the low-price seedling growth medium; the cost is low; environmental protection efficiency is high.

The invention discloses a method for manually culturing rhodiola rosea in a high-altitude area, belonging to the technical field of manual culturing of the rhodiola rosea. A seedling which is subjected to seed dressing with fine sands and sprout culturing with a low bed is taken as a germchit, the rhodiola rosea sapling which grows for 10-12 months in a sprout bed is transplanted into a land in the next March to May, a plant space and a row space of culturing in the land is 20-30cm, and muck is spread over the land after the seedling recovery; and in later each April to June, 1000-1500kg of muck is spread in each mu of land, water control processing is implemented in each August to September, the processing time is 10-30 days, a continuous period is 3-5 years, and the medicinal material of rhodiola rosea is harvested after finishing the process. According to the method, the seedling is subjected to the seed dressing with fine sands, so that seeds are enabled to be uniformly sown, and the quality of the sapling is improved; by adopting the muck to replace fertilizer, the quality of the medicinal material is guaranteed; and by the adoption of water control in a later period, the yield of the medicinal material is increased.

A nucleic acid sequence is provided which encodes a gibberelin 2-oxidase gene which catalyzes the 2β-oxidation of a gibberelin molecule to introduce a hydroxyl group at C-2 and further catalyzes the oxidation of the hydroxyl group introduced at C-2 to yield the ketone derivative. Such sequences can find application in the preparation of transgenic plants with altered levels of gibberelin 2-oxidase.