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Tissue culture rapid-propagation method of beaucarnea recurvata

A tissue culture and wine bottle orchid technology, applied in the field of plant reproduction, can solve the problems of difficulty in harvesting seeds, slow reproduction speed, high price, etc., and achieve the effects of solving factory-based seedling raising, realizing large-scale production, and improving the multiplication coefficient.

Inactive Publication Date: 2015-05-06
GUANGXI BOTANICAL GARDEN OF MEDICINAL PLANTS
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But Agave plants bloom very late, and it is not easy to gather seeds. Domestically cultivated wine bottle orchids are not easy to set seeds, and most of the seeds are imported from abroad, which is expensive; and wine bottle orchids need about 5 years old to bloom, and the propagation speed is extremely slow (Li Yiying , 1996; Lin Yunjia, 2009; Ling Yongjian and Jiang Baodong, 2013)

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] The wine bottle orchid tissue culture rapid propagation method of the present invention comprises the following steps:

[0022] (1) Selection and disinfection of explants: Take the seeds of wine bottle orchid as explants, soak them in 2% aqueous solution of detergent for 5 minutes, rinse them with tap water for 15-20 minutes, add 2-3 drops of Tween -20 100mg / L0.1% mercury liter disinfection for 8 to 10 minutes, rinsed with sterile water for 3 to 5 times, and finally removed the surface moisture with sterile filter paper to obtain explants, in which the sterile water was autoclaved distilled water;

[0023] (2) Seed germination to obtain sterile test-tube plantlets: Inoculate the explants obtained in step (1) into MS germination medium at a temperature of 22-26 degrees, light intensity of 1500 lux, and light time of 8-10 hours / day Cultivate under certain conditions for 35 days, and obtain sterile test-tube plantlets after the seeds germinate. Among them, MS germination ...

Embodiment 2

[0029] The wine bottle orchid tissue culture rapid propagation method of the present invention comprises the following steps:

[0030] (1) Selection and disinfection of explants: Take the seeds of the wine bottle orchid as explants, soak them in 2% aqueous solution of detergent for 5 minutes, wash the linear tap water for 15-20 minutes, add 2-3 drops of Tween -20 100mg / L0.1% mercury liter disinfection for 8 to 10 minutes, rinsed with sterile water for 3 to 5 times, and finally removed the surface moisture with sterile filter paper to obtain explants, in which the sterile water was autoclaved distilled water;

[0031] (2) Seed germination to obtain sterile test-tube plantlets: inoculate the explants obtained in step (1) into MS germination medium at a temperature of 22-26 degrees, light intensity of 1500 lux, and light time of 8-10 hours / day Cultivate under certain conditions for 35 days, and obtain sterile test-tube plantlets after the seeds germinate. Among them, MS germinat...

Embodiment 3

[0037] The wine bottle orchid tissue culture rapid propagation method of the present invention comprises the following steps:

[0038] (1) Selection and disinfection of explants: Take the seeds of the wine bottle orchid as explants, soak them in 2% aqueous solution of detergent for 5 minutes, wash the linear tap water for 15-20 minutes, add 2-3 drops of Tween -20 100mg / L0.1% mercury liter disinfection for 8 to 10 minutes, rinsed with sterile water for 3 to 5 times, and finally removed the surface moisture with sterile filter paper to obtain explants, in which the sterile water was autoclaved distilled water;

[0039] (2) Seed germination to obtain sterile test-tube plantlets: Inoculate the explants obtained in step (1) into MS germination medium at a temperature of 22-26 degrees, light intensity of 1500 lux, and light time of 8-10 hours / day Cultivate for 35 days under certain conditions, and obtain sterile test-tube plantlets after the seeds germinate. Among them, MS germinat...

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Abstract

The invention relates to a tissue culture rapid-propagation method of beaucarnea recurvata. The tissue culture rapid-propagation method comprises the following steps: (1) placing a disinfected beaucarnea recurvata seed as an explant into an MS germination culture medium for germination induction to obtain a sterile test-tube seedling; (2) placing the test-tube seedling into an MS cluster bud culture medium for propagation culture to obtain cluster buds; (3) placing the cluster buds into an MS strong seedling culture medium for culture to obtain a strong plant; (4) placing the strong plant into a 1 / 2MS rooting culture medium for culture to obtain a rooting seedling; (5) carrying out seedling adaptation on the rooting seedling, and transplanting to a sand bed to grow for one month, and then transplanting to a field. According to the tissue culture rapid-propagation method, the multiplication coefficient of the obtained cluster buds is 20-30 times, the rooting rate of an obtained tissue culture seedling is more than 95%, and the survival rate of a transplanting seedbed is more than 90%, so that the problem of industrialized seedling culture of the beaucarnea recurvata is effectively solved.

Description

technical field [0001] The invention relates to a plant propagation method, in particular to a rapid propagation method of wine bottle orchid tissue culture. Background technique [0002] Wine bottle orchid (Nolinare curvata Lem) is a small tree-like succulent herb that is native to the dry and hot region of Northwest Mexico. There are only 2-3 species of plants in the genus Norina, and they have a prominent position in the plant classification system. Because they are relatively difficult to reproduce and not easy to popularize, they are regarded as rare plants. The stem of the wine bottle orchid is upright, the lower part is fat, resembling a wine bottle; Because of its charming and unique posture, sun-loving and shade-tolerant, large plants can be arranged in hotels and other places in addition to exhibitions in botanical gardens; small plants can be matched with flower pots of appropriate shape to decorate meeting rooms, offices and living rooms , very elegant, full of...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 李刚韦坤华王晓峰李竞才舒伟屈啸声缪剑华李林轩
Owner GUANGXI BOTANICAL GARDEN OF MEDICINAL PLANTS
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