Tissue culture method for Punica granatum and culture medium
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A technique for ornamental pomegranate and culture medium, applied in the field of tissue culture method and culture medium of ornamental pomegranate, can solve the problems of high explant contamination rate, low transplant survival rate, low multiplication coefficient, etc. coefficient, the effect of stable seedling system
Inactive Publication Date: 2016-04-06
枣庄市农业科学研究院
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[0003] The purpose of the present invention is to provide a method for tissue culture of ornamental pomegranate, which solves the technical difficulties in the selection of ornamental pomegranate explants, explant collection time, explant disinfection methods, inoculation, culture room cultivation, seedling hardening, etc., and provides A culture medium for ornamental pomegranates, which solves the problems of rapid and large-scale propagation of ornamental pomegranates and makes up for the problems of high explant contamination rate, low differentiation rate, low proliferation coefficient, low transplanting survival rate and high cost in traditional technology
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Embodiment 1
[0053] The preparation of embodiment 1MS medium:
[0054] Concentration of working solution contains (unit: mg / L):
[0059] Iron salt: FeSO 4 ·7H 2 O27.8, Na 2 -EDTA·2H 2 O37.3.
[0060] Amplify the concentration of macroelements to 10 times to make 1L of mother liquor for later use, and amplify trace elements, inositol solution, organic solut...
Embodiment 2
[0067] Example 2 Induction Medium for Ornamental Pomegranate Tissue Culture
[0068] Each mother liquor prepared in Example 1 measures the amount of 100ml of a large number of element mother liquors, and the amount of each 10ml of other mother liquors. -15000mg / L; PH value 5.8-6.0. Mix the above raw materials evenly, dilute to 1L, boil the agar powder, and dissolve the agar powder.
Embodiment 3
[0069] Embodiment 3 Proliferation medium for ornamental pomegranate tissue culture
[0070] Each mother liquor prepared in Example 1 measures the amount of 100ml of a large number of element mother liquors, and the amount of each 10ml of other mother liquors. -15000mg / L; PH value 5.8-6.0. Mix the above raw materials evenly, dilute to 1L, boil the agar powder, and dissolve the agar powder.
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Abstract
The invention discloses a tissue culture method for Punica granatum and a culture medium. The culture method comprises explants picking, induction of differentiated seedlings, proliferation culture of differentiated seedlings, rooting culture of differentiated seedlings and transplantation of tissue culture seedlings to the field. The culture medium comprises an induction medium, a proliferation medium and a rooting medium. A Punica granatum tissue culture induction method is also included. The three mediums are respectively prepared and applied correspondingly. By the method of the invention, propagation coefficient of Punica granatum is raised; some insufficiencies of a traditional tissue culture technology are compensated; contamination rate of explants is reduced; and differentiation rate and transplanting survival rate of buds are enhanced. by the preparation method of the induction medium, the proliferation medium and the rooting medium, culture period of tissue culture seedlings can be shortened, there is no variation, and uniformity of seedlings is high. In addition, proliferation is rapid, propagation coefficient is raised. Rooting rate is high, there is no callus between roots and seedlings, and roots are all normal roots. By the cooperation of the three mediums, survival rate of domestication and proliferation coefficient can be greatly raised, and cost of tissue culture seedlings is reduced.
Description
technical field [0001] The invention belongs to the technical field of fruit tree cultivation agricultural biology, and in particular relates to a tissue culture method and culture medium of ornamental pomegranate. Background technique [0002] Ornamental pomegranate has red flowers and red fruits. It is an excellent variety for viewing flowers and fruits in landscaping. Pomegranate fruit has both high economic value and outstanding beautification effect. Ornamental pomegranate fruit is red in color, full in fruit, rich in fruit, large in quantity, high in pomegranate yield, hard to fall off after fruit ripening, can play a good ornamental effect, and can be viewed for a long time. Therefore, the demand for ornamental pomegranates has increased dramatically. At present, the main propagation method of ornamental pomegranates is cutting. However, the propagation speed of cuttings is slow and is greatly affected by the seasons. It cannot form industrialized and standardized p...
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