Bartonia body solid-liquid double-phase slant culture medium as well as preparation method and application method thereof
A technology of slant culture medium and solid culture medium, applied in microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve the problems of inability to study bacterial secreted proteins, long growth cycle and high cost
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Embodiment 1
[0024] Embodiment 1 Bartonella henselae (Bartonella henselae) solid-liquid two-phase medium configuration and cultivation
[0025] (1) Blood agar solid slant medium and blood agar plate medium
[0026] Beef extract 23.0g, starch 1.0g, sodium chloride 5.0g, agar 12.0g, water 1L, heated to 50°C to promote its dissolution. Pack into appropriate Erlenmeyer flasks and autoclave at 121°C for 15 minutes.
[0027] Preparation of defibrinated sheep blood: Collect 100ml of venous blood from healthy sheep, pour it into a sterile beaker, add 20 sterilized glass beads, shake at 120rpm / min for 10min, discard the glass beads, and store the blood at 4°C until use.
[0028] After the culture medium was cooled to 50°C, a total amount of 5% defibrinated sheep blood was added, and 3ml of each tube was poured into a sterilized test tube and placed obliquely to make a blood agar slant medium.
[0029] (2) Casein Trypsin Hydrolyzed Soybean Liquid Medium
Embodiment 2
[0036] Embodiment 2 Bacillus-like Bartonella (Bartonella bacilliformis) solid-liquid biphasic medium configuration and cultivation
[0037] (1) Blood agar solid slant medium and blood agar plate medium
[0038] 30.0g of beef extract, 1.0g of starch, 5.0g of sodium chloride, 12.0g of agar, and 1000ml of water are heated to promote their dissolution. Pack into appropriate Erlenmeyer flasks and autoclave at 121°C for 15 minutes.
[0039] Preparation of defibrinated sheep blood: Collect 100ml of venous blood from healthy sheep, pour it into a sterile beaker, add 20 sterilized glass beads, shake at 120rpm / min for 10min, discard the glass beads, and store the blood at 4°C until use.
[0040] After the culture medium was cooled to 50°C, a total amount of 8% defibrinated sheep blood was added, and 3ml of each tube was poured into a sterilized test tube and placed obliquely to make a blood agar slant medium.
[0041] (2) Casein Trypsin Hydrolyzed Soybean Liquid Medium
[0042] Casei...
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