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Blood enriched culture bottle for full automatic blood culture system and preparation thereof

A blood culture and enrichment culture technology, applied in the field of blood enrichment culture bottles and its preparation, can solve the problems of blood culture difficulties, different detection principles, medical expenses burden, etc., to simplify production operations, eliminate inhibition, reduce The effect of testing costs

Inactive Publication Date: 2009-01-28
天津市鑫康赛生物技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the high cost of testing brought about by the blood culture bottles matched with imported instruments has brought a heavy burden on the medical expenses of the people
Moreover, the culture bottle enrichment principle of the two major brands of blood culture systems is the same, but the detection principle is different, and the versatility is poor
[0003] In addition, according to my country's national conditions, most patients with bacteremia have used antibiotics before blood culture, which brings difficulties to ordinary blood culture

Method used

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  • Blood enriched culture bottle for full automatic blood culture system and preparation thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Weigh TSB 20.75g, yeast extract 10g, glucose 0.5g, sucrose 0.56g, hemin 0.005g, vitamin K3 0.00025g, vitamin B6 0.01g, SPS 5g, antibiotic neutralizer p-aminobenzoic acid 10g, add 1L of distilled water was boiled and dissolved, poured into a 1000mL graduated cylinder after cooling to supplement the evaporated water, and the pH value was adjusted to 7.2.

[0025] The silicone rubber JG-T17 was mixed in a ratio of 10:1, added with 1% bromocresol violet indicator with pH=8, stirred evenly with an electronic stirrer, and poured into a glass bottle to form a 5mm thick sensing layer. Bubble for 30min and cure at 60℃ for 1h.

[0026] Add 3 g of pretreated resin SAD-2, 1 g of LS-33, and 25 mL of culture medium to the bottle, seal and sterilize.

Embodiment 2

[0028] Weigh TSB 20g, yeast extract 2.5g, glucose 1g, sucrose 3g, hemin 0.0025g, vitamin K3 0.00025g, vitamin B6 0.1g, SPS 0.25g, antibiotic neutralizer Tween 80 2g, add distilled water or 500mL of deionized water was boiled and dissolved, and after cooling, poured into a 500mL graduated cylinder to replenish the evaporated water, and the pH value was adjusted to 7.2.

[0029] Silicone rubber Sylgard184 was mixed in a ratio of 10:1, added with 1% cresyl red and 1% para-rose bengal at pH=9, stirred evenly with an electronic stirrer, poured into a glass bottle to form a 5mm thick sensing layer, and left to stand for defoaming 30min, curing at 60℃ for 1h.

[0030] Add 3 g of pretreated resin SAD-2, 001×4 1 g, and 25 mL of culture medium to the bottle, seal and sterilize.

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Abstract

The invention discloses a blood bacteria proliferation culture bottle used for a full automatic blood culture system, including: a bottle body of a sealed culture bottle, a sensing level arranged at the bottom of the bottle body, and polymeric adsorbent used for adsorbing antibiotic and culture medium which are placed on the sensing level, wherein, the culture medium includes 5 to 75g / L of TSB, 0.5 to 30g / L of yeast extract, 0.1 to 20g / L of glucose, 0.01 to 20g / L of sucrose, 0.001 to 0.5g / L of chlorhematin, 0.0001 to 0.05g / L of Vitamin K3, 0.001 to 5g / L of Vitamin B6, 0.1 to 30g / L of SPS, 1 to 75g / L antibiotic neutralizing agent, and the residue is distilled water or deionized water; and the sensing level contains 1 to 100g / L of an indicator. The culture bottle of the invention can eliminate the inhibitory action of the residual antibiotic in the blood to the growth of the microorganism, and simplify the production operation.

Description

technical field [0001] The invention relates to the technical field of in vitro diagnosis, in particular to a blood enrichment culture bottle used in an automatic blood culture system and a preparation method thereof. Background technique [0002] Rapid and accurate detection of pathogenic microorganisms in blood has always been the goal of research in the field of in vitro detection technology. After going through manual and semi-automatic processes, a computer-controlled automatic blood culture system was fully developed in the 1990s. Representative products such as American BD The company and the French company Mérieux's fully automated blood culture system. With the development of my country's economy, this advanced system has gradually entered the testing departments of domestic hospitals, improving the detection rate of pathogenic microorganisms in blood. However, the high testing cost of blood culture bottles matched with imported instruments has brought a heavy burd...

Claims

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Application Information

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IPC IPC(8): C12M1/24C12Q1/04G01N21/78G01N21/64
Inventor 刘学源
Owner 天津市鑫康赛生物技术有限公司
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