Method for preparing xylonic acid (salt) through whole-cell high-efficiency catalysis of xylose transformation

A high-efficiency catalysis of xylose and xylonic acid, which is applied in the field of efficient whole-cell catalysis of xylose conversion to produce xylonic acid (salt), to achieve the effect of increasing the reaction concentration

Inactive Publication Date: 2012-09-19
NANJING FORESTRY UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Therefore, the existing chemical synthesis and enzymatic conversion of xylose to produce xylonic acid (salt) do not have the conditions for industrial production, and the microbial fermentation method still needs to further improve the substrate and product concentrations, raw material utilization and conversion of the reaction system. In order to ensure the overall economic benefits of industrialized production of xylose and even lignocellulosic raw material biorefinery

Method used

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  • Method for preparing xylonic acid (salt) through whole-cell high-efficiency catalysis of xylose transformation
  • Method for preparing xylonic acid (salt) through whole-cell high-efficiency catalysis of xylose transformation

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] In a 3.0L fully automatic mechanically stirred ventilated fermenter, add 1500mL of xylose solution with an initial xylose mass concentration of 9.5%, add 0.4 g / L magnesium sulfate, 1.5 g / L dipotassium hydrogen phosphate and 1.0 g / L sulfuric acid ammonium, control the stirring rate to 300r / min, pass through sterile compressed air until the dissolved oxygen concentration is 10%~50%, insert the activated Gluconobacter oxidans NL71 until the cell concentration is OD 600nm = 1.0, add powdered calcium carbonate to adjust the pH of the reaction system to about 6.5, and carry out the whole-cell catalytic reaction.

[0021] Monitor the pH value and xylose concentration of the reaction system in real time, add about 100g of powdery solid xylose and calcium carbonate in 3 batches every 20~24h, control the pH value to 3.0~6.5 and the xylose mass concentration to not exceed 18% (g / mL).

[0022] Such as figure 1 , reacted to 192h, the mass concentration of xylose in the reaction ...

Embodiment 2

[0024] In a 10.0L fully automatic mechanical ventilated stirring fermenter, add 4L of xylose solution with an initial xylose mass concentration of 15%, add 0.4 g / L magnesium sulfate, 1.5 g / L dipotassium hydrogen phosphate and 1.0 g / L sulfuric acid ammonium, control the stirring rate to 280r / min, pass through sterile compressed air until the dissolved oxygen concentration is 10%~50%, and insert the activated ATCC 621-H strain until the cell concentration is OD 600nm = 1.1, add powdered calcium carbonate to adjust the pH of the reaction system to about 6.5, and carry out the whole-cell catalytic reaction.

[0025] The pH value and xylose concentration of the reaction system are monitored in real time, solid xylose is added continuously, and the pH value is controlled in real time to be 3.0-6.5 and the xylose mass concentration is not higher than 25%.

[0026] Add to the reaction system the cumulative mass concentration of xylose added to reach 30%, stop adding, react until the ...

Embodiment 3

[0028] In a 100 L fully automatic mechanical ventilated stirring fermenter, add 70 L of xylose solution with an initial xylose mass concentration of 14%, add 0.4 g / L magnesium sulfate, 1.5 g / L dipotassium hydrogen phosphate and 1.0 g / L sulfuric acid Ammonium, control the stirring rate to 220r / min, feed sterile compressed air to the dissolved oxygen concentration of 15%~40%, and insert the activated ATCC 621-H strain until the cell concentration is OD 600nm = 1.1, add powdered calcium carbonate to adjust the pH of the reaction system to about 6.5, and carry out the whole-cell catalytic reaction.

[0029] Monitor the pH value and xylose concentration of the reaction system in real time, add high-concentration xylose solution in batches, and control the pH value to 3.0-6.5 and the xylose mass concentration not higher than 20% in real time.

[0030] Added to the reaction system, the cumulative mass concentration of xylose added reached 29.2%, the addition was stopped, and the react...

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Abstract

The invention discloses a method for preparing xylonic acid (salt) through whole-cell high-efficiency catalysis of xylose transformation. The method comprises the following steps of: adding an initial reaction matrix into a liquid deep-layer ventilation reaction system; inoculating gluconabacteriumoxydonas; stirring and ventilating to continuously react; keeping dissolved oxygen concentration of the reaction system not less than 10 percent; adding xylose or xylose solution into the reaction system in a semi-continuous or continuous batch adding mode; controlling mass concentration of xylose in the reaction system to be not greater than 25 percent; synchronously adjusting and controlling the pH value to be not less than 2.8; stopping adding when the mass concentration of the xylose accumulatively added into the reaction system reaches 30 percent; and continuously performing full catalytic reaction to obtain a transformed product, i.e., the xylose acid (salt) product. According to the method, the maximum value of the accumulative concentration of the substrate xylose can reach 30 percent, the maximum utilization rate of the xylose exceeds 99 percent, the maximum concentration value of the fermentation product, i.e., the xylose acid (salt), can reach 29.8 percent, and the transformation ratio of the xylose acid (salt) can reach 100 percent.

Description

technical field [0001] The invention relates to a preparation method of xylonic acid (salt), in particular to a method for preparing xylonic acid (salt) by efficiently catalyzing xylose conversion in whole cells. Background technique [0002] The efficient conversion and utilization of xylose is a key technical bottleneck in the processing and utilization of lignocellulosic raw materials, especially in its biorefinery. At present, the main products of xylose bioconversion are fuel ethanol, xylitol, xylose acid (salt), lactic acid, succinic acid and feed yeast. Except for xylonic acid (salt), due to the limitation of market capacity, environmental pollution and existing production technology level, the development space of other products is very limited at present. [0003] Xylonic acid (salt) has similar properties to gluconic acid (salt), and can be used as a substitute for products such as citric acid and gluconic acid. As an emerging bio-based chemical, its use has been ...

Claims

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Application Information

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IPC IPC(8): C12P19/02C12R1/01
Inventor 徐勇勇强余世袁王荥
Owner NANJING FORESTRY UNIV
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