Dual Tem-PCR quick detection method for salmonella and escherichia coli O78

Abstract

The invention discloses a dual Tem-PCR quick detection method for salmonella and escherichia coli O78. According to the dual Tem-PCR quick detection method for the salmonella and the escherichia coli O78, invA gene primer of the salmonella and O-antigen gene primer of the escherichia coli are used for conducting dual Tem-PCR amplification directly on extracted mixing DNA after bacteria enrichment is conducted on a complex sample, and the result is interpreted through gel electrophoresis. A Tem-PCR technology and a dual PCR technology are combined, the problem of enrichment preference of the PCR is solved, the sensitivity degree is improved by 2 to 3 orders of magnitudes compared with an ordinary dual PCR detection, the detection level that the number of sample contaminated bacteria is a single digit can be reached, and the problem that a traditional dual PCR technology is prone to lead to false negative result. According to the dual Tem-PCR quick detection method for the salmonella and the escherichia coli O78, the salmonella and the escherichia coli O78 can be detected quickly, conveniently, excellently and sensitively, wide-range popularization is easy, the salmonella and the escherichia coli O78 can be used for bacteria identification, disease diagnosis and epidemiological investigation, and wide market prospect and comparatively large economic benefits are achieved.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a double Tem-PCR rapid detection method and application of Salmonella and Escherichia coli O78. Background technique [0002] Rapid detection and identification of pathogenic bacteria is the prerequisite for timely and effective prevention and control of the spread of pathogenic bacteria. It is of great significance to the healthy and normal development of the livestock and poultry industry, food inspection and quarantine safety, and human health. [0003] At present, pathogenic bacteria in food sanitation microbiology inspection (GB / T 4789.1-2003) still mainly rely on traditional bacterial culture, serology, biochemical identification and other methods. Both are low and cannot meet the needs of rapid detection. With the development of nucleic acid-based molecular microorganism detection technology, especially the application of polymerase chain reaction (Polymerase Chai...

AI Technical Summary

Problems solved by technology

Only the concentration of super primers is sufficient for exponential amplification, which overcomes the amplification bias problem of multiplex PCR, improves the detection sensitivity of traditional multiplex PCR by 2-3 orders of magnitude, and solves the problem of false negative results caused by traditional multiplex PCR technology

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