The invention discloses a vertical multi-layer closed
nuclear polyhedrosis virus multiplication process, which is accomplished by the following steps: 1, selecting a land, which has an area of more than or equal to 50m<2> and has naturally or artificially
growth plant feed around and a
water source nearby, as a multiplication room; 2, building a vertical trapezoid platform in the multiplication room for breeding larvae; 3, closing the
doors and windows of the multiplication room; 4, collecting 2-2.5-year-old larvae of
hyphantria cunea and placing the larvae on the multiplication platform flatly; 5, spraying viral liquid onto the placed larvae of
hyphantria cunea; and 6, collecting corpses of larvae dead of diseases in a container containing
sterile water to soak the corpses for 2 to 4 days,
grinding and crushing the corpses in a homogenizing
crusher to obtain homogenate, filtering the homogenate with a gauze element, subjecting residue filtered out to secondary
grinding, filtering with the gauze element, and obtaining
hyphantria cunea polyhedrosis
virus liquid. The process technique is simple, can improve the number of multiplied viruses, reduce the cost of
virus multiplication, facilities the collection of dead larvae infected with the
virus and is suitable to be promoted and used in vast rural areas.