Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Fluorescent quantitative reference genes of hyphantria cunea adults with different sexes as well as primers and application of fluorescent quantitative reference genes

A kind of American white moth, fluorescence quantitative technology, applied in the application, genetic engineering, plant genetic improvement and other directions, can solve the problem of relative expression quantitative research, to achieve the effect of improving repeatability, strong specificity and high amplification efficiency

Pending Publication Date: 2021-12-10
南京市园林和林业科学研究院(南京市园林绿化指导站)
View PDF8 Cites 1 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Therefore, it is necessary to screen the stability of internal reference genes under adults of different sexes in this pest, otherwise it is impossible to conduct quantitative research on the relative expression of functional genes in adults of different sexes in this pest

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Fluorescent quantitative reference genes of hyphantria cunea adults with different sexes as well as primers and application of fluorescent quantitative reference genes
  • Fluorescent quantitative reference genes of hyphantria cunea adults with different sexes as well as primers and application of fluorescent quantitative reference genes
  • Fluorescent quantitative reference genes of hyphantria cunea adults with different sexes as well as primers and application of fluorescent quantitative reference genes

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] 1. Total RNA extraction and cDNA synthesis of adult white moths of different sexes

[0026] White moths fed by adults of different sexes were collected. Each treatment had three biological replicates, and they were frozen in liquid nitrogen. After the treatment, total RNA was extracted by Trizol method, and 5 mL of RNase-free DNase I (TakaraBio Inc. , Kusatsu, Shiga Prefecture, Japan) for 15 minutes to remove DNA. The concentration and quality of RNA were detected using a ThermoScientific NanoDrop 2000 UV-Vis spectrophotometer (Thermo Fisher Scientific Inc., Waltham, MA, USA). The integrity of total RNA was detected by 1.0% agarose gel electrophoresis. Using PrimeScript TM II first-strand cDNA synthesis kit (TaKaRa, Dalian, China), purify RNA and synthesize the first-strand cDNA according to the instructions. Perform reverse transcription in a 20.0 μL reaction system containing 4.0 μL 5XPrimeScript IV cDNA Synthesis Mix, 1.0 μL random primer (50 μM), 4.0 μL total RNA...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses fluorescent quantitative reference genes of hyphantria cunea adults with different sexes as well as primers and application of the fluorescent quantitative reference genes, and belongs to the field of molecular biology of forestry insects. The fluorescent quantitative reference genes are RPL13 and RPS15 genes, and the nucleotide sequences of the fluorescent quantitative reference genes are shown as SEQ ID NO. 1 and SEQ ID NO. 2. According to a transcriptome sequencing database of hyphantria cunea, eight candidate reference genes commonly used as reference genes are selected, the stability of the candidate reference genes is evaluated through five algorithms, the fluorescent quantitative reference genes of hyphantria cunea adults with different sexes are obtained, the real-time fluorescent quantitative PCR primers of the reference genes are designed, and the primers are high in specificity, and high in amplification efficiency. The current situation that the hyphantria cunea adults with different sexes have no suitable reference genes is filled up, and a reliable analysis basis is provided for expression quantification of related functional genes of the hyphantria cunea under different sex experiment conditions.

Description

technical field [0001] The invention belongs to the field of forestry insect molecular biology. It specifically relates to an internal reference gene for fluorescence quantification of adult white moths of different sexes, primers and applications thereof. Background technique [0002] The American white moth (Hyphantria cunea (Drury)) belongs to Lepidortera of Lepidoptera and Arctiidae of the family Arctiidae. It is a worldwide quarantine pest and is listed as one of the first invasive alien species in my country. It has a wide range of hosts, and has the characteristics of miscellaneous food habits, fast transmission, and rampant harm. It mainly harms fruit trees, street trees and ornamental trees, especially broad-leaved trees, including sugar maple, mulberry, elm, ailanthus, willow, hawthorn, apricot, and sycamore. It is harmful to eat leaves, which seriously affects the normal growth of plants, fruit and vegetable yield and quality. At the same time, it will cause se...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C12N15/12C12Q1/6879C12Q1/6888C12Q1/6851C12N15/11
CPCC07K14/43563C12Q1/6879C12Q1/6888C12Q1/6851C12N15/11C12Q2600/166C12Q2531/113C12Q2545/101C12Q2563/107
Inventor 陈文霞黄志宽
Owner 南京市园林和林业科学研究院(南京市园林绿化指导站)
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com