Method for screening active component with function of hexokinase inhibition effects
A technology of hexokinase and inhibition, applied in the field of screening of active ingredients of traditional Chinese medicines, can solve the problems of tumor cell apoptosis, inability to obtain glucose 6-phosphate, etc., and achieve the effects of less dosage, saving of biochemical reagents, and rapid screening
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[0027] Example 1: Screening of Hexokinase Inhibitory Active Components in the Extract of Squilla Sinensis
[0028] 1. Determination of ATP and ADP by LC-MS
[0029] Chromatographic column: Agilent Extend C-18 (3.1×100mm, 3μm; Agilent, Palo Alto, CA, USA); mobile phase: 5mM ammonium acetate (pH7.5):methanol=6:4; flow rate: 0.3mL / min; Column temperature: 20°C; Injection volume: 10μL
[0030] Ion source: liquid chromatography-mass spectrometry interface: electrospray interface (ESI), positive ion mode (positive ion); drying gas flow rate: 10mL / min; drying gas temperature: 300°C; atomizing gas pressure: 30psi (upper limit 60psi); capillary voltage: 3000V; mass analyzer: detection method: selected ion monitoring (SIM); detection interval: 0-1.6min; fragment voltage: 100V; monitoring ion: ATP, quasi-molecular ion peak (m / z 508) ;ADP, quasi-molecular ion peak (m / z428)
[0031] Specific as figure 1 As shown, the ATP retention time is 1.39 minutes, and the ADP retention time is 1.4...
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