40 results about "Phosphoglucomutase" patented technology

The invention discloses a method for preparing tagatose by using whole-cell catalysis. The method comprises the following steps of transferring plasmid containing an alpha-glucan phosphorylase gene (or cellulosic polysaccharide phosphorylase and cellobiose phosphorylase genes, or a sucrose phosphorylase gene), a phosphoglucomutase gene, a glucose phosphate isomerase gene, a 6-phosphate tagatose epimerase gene, and a 6-phosphate tagatose phosphatase gene into engineered escherichia coli, so as to obtain converted bacterial strain; inducing the converted bacterial strain to produce enzyme, and permeabilizing; mixing the permeabilized bacterial strain, establishing a whole-cell enzyme catalysis reaction system with starch or starch derivative (or mixture of cellulase and cellulose or cellulose derivative, or sugarcane), and performing the whole-cell enzyme catalysis reaction, so as to obtain the tagatose. The method has the advantages that the tagatose is prepared by the whole-cell catalysis; the cost is low, the pollution is low, the yield rate is high, and the like; the method is suitable for preparing the tagatose in a scale way.

The invention discloses bacillus subtilis genetically-engineered bacteria for producing tagatose and a method for preparing the tagatose. The genetically-engineered bacteria are used for constructingheat-resistant alpha-glucan phosphorylase, heat-resistant phosphoglucomutase, heat-resistant glucose phosphate isomerase, heat-resistant 6-tagatose phosphate epimerase and heat-resistant 6-tagatose phosphate phosphatase which are independently expressed or co-expressed. By utilizing the genetically-engineered bacteria, starch can be effectively converted into the tagatose. Compared with the existing method for producing the tagatose, the method disclosed by the invention has the advantages of suitability for whole-cell recycling, high safety, high yield, simple production process, low cost, easiness in large-scale preparation and the like.

The invention discloses a method for improving the content of cordyceps polysaccharide in cordyceps militaris. The method comprises the following steps of: inoculating cordyceps militaris recombinantbacteria containing a target gene into a fermentation culture medium, and carrying out shake cultivation at a temperature of 22-25 DEG C at 120-180 rpm to obtain fermentation liquor containing the cordyceps polysaccharide, wherein the target gene is a glucokinase gene gk, a glucophosphomutase gene psm or a pyrophosphorylase gene ugp. Through a transgenic technology, the gene mutation bacterial strain of the cordyceps militaris is obtained. Compared with an original bacterial strain, the mutation bacterial strain is characterized in that the yield of the cordyceps polysaccharide in the fermentation liquor of the mutation bacterial strain is 1.35 times, and a foundation is laid for further screening a good variety of the cordyceps militaris.

The invention discloses a glmM gene knock-out bacterial strain ML2009 (mycobacterium smegmatis), CGMCC (China General Microbiological Culture Collection Center) 3418, which is constructed by using phosphoglucomutase participating in the biosynthesis of key components in a mycobacterium tuberculosis cell wall. The bacterial strain ML2009 can be used as a cell model for sieving phosphoglucomutase inhibitors with high flux, be used for sieving effective phosphoglucomutase inhibitors from a combined compound library, traditional Chinese medicine and natural products to prepare tuberculosis-resisting medicaments with high medicine effects; and in addition, in the cells of a human body, the synthesis approach of UDP (Uridine Diphosphate)-acetyl glucosamine is different from that of mycobacterium tuberculosis, no phosphoglucomutase exists in the UDP-acetyl glucosamine, therefore, the reaction catalyzed by the mycobacterium tuberculosis phosphoglucomutase does not exist in the cells of the human body so that the tuberculosis-resisting medicaments developed by using the phosphoglucomutase as a target enzyme are harmless to the human body, and the defect that the traditional antibacterial medicament also kill normal cells is overcome.

The invention provides a method for planting Ganoderma Lucidum. The method comprises: strain culture, culture medium preparation, mycelia culture, and sprouting management. The culture medium preparation includes mixing, grinding, sacking, and sterilizing the following raw materials by part of weight: 30-35 mixed sawdust by parts of weight, 10-20 corncob by parts of weight, 5-10 rice bran by parts of weight, 1-3 burdock by parts of weight, 2-4 gastrodia elata by parts of weight, and 0.5-1.5 lactose by parts of weight. The adding of gastrodia elata, burdock, and lactose to the regular culture medium can increase activity of [alpha]-Phosphoglucomutase, promote metabolism of Ganoderma Lucidum cells to migrate more towards a branch synthesized by Ganoderma Lucidum polysaccharide, which greatly increases content of Ganoderma Lucidum exopolysaccharide, and at the same time promotes growth of Ganoderma Lucidum, and greatly shortens time for sacking mycelia.

The invention provides a ganoderma lucidum cultivation method capable of increasing the content of ganoderma lucidum polysaccharide. The ganoderma lucidum cultivation method comprises the steps of strain cultivation, culture medium preparation, hypha cultivation and ganoderma lucidum emergence management, wherein the culture medium preparation is characterized by mixing, smashing, bagging and sterilizing the following raw materials in parts by weight: 30 to 35 parts of mixed wood chips, 10 to 20 parts of corncobs, 5 to 10 parts of rice bran, 1 to 3 parts of grifola frondosa and 2 to 4 parts of fructus arctii. According to the ganoderma lucidum cultivation method provided by the invention, the fructus arctii and the grifola frondosa are added in a conventional culture medium, so that the activity of alpha-phosphoglucomutase can be increased, the metabolism of ganoderma lucidum cells is facilitated to more migrate towards a branch compounded by the ganoderma lucidum polysaccharide, and the content of exopolysaccharide of ganoderma lucidum can be greatly increased; meanwhile, the growth of the ganoderma lucidum can be promoted, and the full bagging time of hypha is greatly shortened.

The invention relates to the field of enzymatic catalytic preparation of inositol, in particular to an enzymatic preparation method for producing inositol by complete phosphorolysis of cellulose. According to the preparation method of inositol, pretreated cellulose (the main component is fiber polysaccharide) is used as a substrate for the enzyme catalytic reaction; and cellulose polysaccharide phosphorylase, cellobiose phosphorylase, polyphosphate glucokinase, phosphoglucomutase, inositol-1-phosphate synthase and inositol monophosphatase are adopted to catalyze complete phosphorolysis of cellulose polysaccharide into inositol. By optimizing the multienzyme reaction process and controlling the reaction temperature in stages, the conversion efficiency of the substrate and the inositol yieldare significantly improved, and the reaction time is shortened. The technical method realizes the complete phosphorolysis of the cellulose substrate, and can also be used for other in-vitro multi-enzyme reaction systems to catalyze cellulose polysaccharide for production of hydrogen, electricity or other bio-based chemicals.

The invention belongs to the biotechnical field, particularly belongs to the field of genetic engineering, and particularly relates to a gene for encoding phosphoglucomutase in kelp, a protein and applications of the gene. The encoded gene is named as SjaPGM2 gene, the sequence of which is shown as SEQ ID NO:1, the encoded protein is named as SjaPGM2 protein, the sequence of which is shown as SEQ ID NO:2, and is a PGM. The SjaPGM2 protein also has higher catalytic activity compared with the prior art, more excellent metal ion stability and thermal stability, milder optimal reaction temperature, higher catalytic efficiency and more applicable to improvement of plant nutrition quality compared with the prior art, thus providing a very suitable gene and protein resources for the genetic engineering application of PGM.

The invention discloses a detection reagent containing a phosphoglucomutase 5-antibody and application of the detection reagent. The immunohistochemical detection reagent for prostatic cancer diagnosis is provided. According to the detection reagent, an antibody specific to human PGM5 proteins is used as a first antibody, a rabbit 2-antibody marked with HRP (horseradish peroxidase) and other chemical markers is used as a second antibody, and the expression quantity of target antigens (PGM5) in histocytes of a to-be-detected sample is detected through color development and other functions. Byuse of the detection reagent, the expression of PGM5 genes in the histocytes at the protein level can be effectively discriminated; and the diagnosis of a prostatic cancer is judged by detecting the expression quantity of the PGM5 proteins in the histocytes, and therefore a new basis is provided for early diagnosis of the prostatic cancer.

Kits and methods for measuring lithium ions using phosphoglucomutase are disclosed.

The present invention comprises an in vitro enzymatic process that effectively converts renewable polysaccharides into high yields of hydrogen at mild conditions, using only enzymes and water. The process comprises a number of enzymes: (1) phosphorylases, (2) phosphoglucomutases, (3) hydrogenases, and (4) enzymes involved in the pentose-phosphate pathway. Preferred embodiments of the process produce only hydrogen and carbon dioxide as net products, translating into an inexpensive method of generating hydrogen in very large quantities from low-cost feedstocks.

The invention discloses an escherichia coli recombinant bacterium for synthesizing inositol by efficiently utilizing glucose as well as a construction method and application of the escherichia coli recombinant bacterium. The construction method of the escherichia coli recombinant bacterium for producing the inositol, provided by the invention, comprises the steps: introducing an inositol-3-phosphate synthetase gene and an inositol monophosphate enzyme gene into host bacteria to obtain the escherichia coli recombinant bacteria for producing the inositol, wherein the host bacteria are wild escherichia coli or mutant escherichia coli; and the mutant escherichia coli is obtained by knocking out a glucose-6-phosphate isomerase gene or a glucophosphomutase gene or a glucose-6-phosphate dehydrogenase gene in an SG104 genome of the mutant escherichia coli. Glucose can be efficiently utilized to synthesize the inositol by utilizing the escherichia coli recombinant strain for producing the inositol, and the yield of the inositol can be up to 375 mM (67.5 g/L). For inositol production, huge economic benefits can be generated, and the method has great popularization and application values.