Detection reagent containing phosphoglucomutase 5-antibody and application of detection reagent

A technology of phosphate dislocation and detection reagents, which is applied in the field of medical diagnosis and can solve problems such as the undisclosed association of early diagnosis of prostate cancer

Inactive Publication Date: 2019-07-19
SUZHOU MUNICIPAL HOSPITAL
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there is no patent or article disclosing that PGM5 can diagnose prostate cancer, and i...

Method used

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  • Detection reagent containing phosphoglucomutase 5-antibody and application of detection reagent
  • Detection reagent containing phosphoglucomutase 5-antibody and application of detection reagent
  • Detection reagent containing phosphoglucomutase 5-antibody and application of detection reagent

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Fabrication and detection of prostate tissue slices

[0025] 1. Take pathological paraffin sections of prostate tissue;

[0026] 2. Baking slices: Put the tissue slices into the oven, adjust the temperature to 63 degrees, and bake the wax for one hour;

[0027] 3. Dewaxing: After the film is baked, take it out of the oven and put it into an automatic dyeing machine for dewaxing; the dewaxing process is as follows:

[0028] Two cylinders of xylene, 15 minutes per cylinder;

[0029] Two cylinders of absolute ethanol, 7 minutes per cylinder;

[0030] 1 cylinder of 90% alcohol, 5 minutes;

[0031] 1 cylinder of 80% alcohol, 5 minutes;

[0032] 1 cylinder of 70% alcohol, 5 minutes;

[0033] 4. Antigen retrieval: Take out the slices from the staining machine, rinse with pure water 3 times, each time not less than 1 minute. During the washing process, put the antigen retrieval solution on the induction cooker and start heating;

[0034] Preparation of Antigen Retrieval ...

Embodiment 2

[0049] Fabrication and detection of prostate tumor tissue slices

[0050] 1. Take pathological paraffin sections of prostate tissue;

[0051] 2. Baking slices: Put the tissue slices into the oven, adjust the temperature to 63 degrees, and bake the wax for one hour;

[0052] 3. Dewaxing: After the slices are baked, take them out of the oven and put them into an automatic dyeing machine for dewaxing; the dewaxing process is as follows:

[0053] Two cylinders of xylene, 15 minutes per cylinder;

[0054] Two cylinders of absolute ethanol, 7 minutes per cylinder;

[0055] 1 cylinder of 90% alcohol, 5 minutes;

[0056] 1 cylinder of 80% alcohol, 5 minutes;

[0057] 1 cylinder of 70% alcohol, 5 minutes;

[0058] 4. Antigen retrieval: Take out the slices from the staining machine, rinse with pure water 3 times, each time not less than 1 minute. During the washing process, put the antigen retrieval solution on the induction cooker and start heating;

[0059] Preparation of Antig...

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PUM

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Abstract

The invention discloses a detection reagent containing a phosphoglucomutase 5-antibody and application of the detection reagent. The immunohistochemical detection reagent for prostatic cancer diagnosis is provided. According to the detection reagent, an antibody specific to human PGM5 proteins is used as a first antibody, a rabbit 2-antibody marked with HRP (horseradish peroxidase) and other chemical markers is used as a second antibody, and the expression quantity of target antigens (PGM5) in histocytes of a to-be-detected sample is detected through color development and other functions. Byuse of the detection reagent, the expression of PGM5 genes in the histocytes at the protein level can be effectively discriminated; and the diagnosis of a prostatic cancer is judged by detecting the expression quantity of the PGM5 proteins in the histocytes, and therefore a new basis is provided for early diagnosis of the prostatic cancer.

Description

technical field [0001] The invention relates to the field of medical diagnosis, in particular to a detection reagent containing phosphoglucomutase 5 and an application thereof. Background technique [0002] Prostate cancer (PCa) is one of the most common malignant tumors of the male genitourinary system, ranking sixth among cancer-related causes of death, with an estimated 914,000 new cases and 258,000 deaths worldwide each year. The incidence of prostate cancer has obvious differences among regions and races. At present, the incidence of prostate cancer in my country is far lower than that of developed countries in Europe and the United States. However, with the aging of society, changes in people's lifestyles, and the application of new diagnostic technologies, Especially with the introduction of prostate specific antigen (PSA) detection, the incidence of prostate cancer in my country has a significant increase trend, and it has been regarded as a common disease. PSA enter...

Claims

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Application Information

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IPC IPC(8): G01N33/574G01N33/535
CPCG01N33/535G01N33/57434
Inventor 王建青樊彩斌孙健蔡政张珂赵春春
Owner SUZHOU MUNICIPAL HOSPITAL
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