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Serum glucose detection reagent

A technology for detecting reagents and glucose, applied in the field of glucose detection, can solve the problems of poor stability of ATP and related enzymes, and achieve the effects of enhancing anti-interference ability, high thermodynamics, and improving activity and stability

Active Publication Date: 2015-11-18
郁东
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the stability of the reaction substrate ATP and related enzymes is slightly poor, and it has become an urgent task to solve the stability of the hexokinase method reagents.

Method used

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  • Serum glucose detection reagent
  • Serum glucose detection reagent
  • Serum glucose detection reagent

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Embodiment 1

[0048] A serum glucose detection reagent is composed of reagent R1 and reagent R2;

[0049] The reagent R1 is composed of the following components:

[0050] Buffer 100mmol / L

[0051] Mg 2+ 5mmol / L

[0052] Adenosine triphosphate (ATP) 2mmol / L

[0053] NAD+3mmol / L

[0054] Non-ionic fluorocarbon surfactant 3ml / L

[0055] BSA2g / L

[0056] Sucrose 20g / L

[0057] Trehalose 5g / L

[0058] Mannitol 20g / L

[0059] NaN 3 0.5g / L;

[0060] Described reagent R2 is made up of the composition of following content:

[0061] Buffer 100mmol / L

[0062] Mg 2+ 5mmol / L

[0063] BSA2g / L

[0064] Sucrose 20g / L

[0065] Trehalose 5g / L

[0066] Mannitol 20g / L

[0067] Glucose-6-phosphate dehydrogenase 2KU / L

[0068] Hexokinase 2KU / L

[0069] Fluorocarbon surfactant 3ml / L

[0070] NaN 3 0.5g / L.

[0071] Said, the volume ratio of R1 reagent and R2 reagent is 4:1.

[0072] Said buffer is piperazine-1,4-diethanesulfonic acid buffer at pH 7.5 at 25°C.

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Abstract

The invention discloses a serum glucose detection reagent which is composed of a reagent R1 and a reagent R2, wherein the reagent R1 is composed of a buffer solution, Mg<2+>, adenosine triphosphate (ATP), NAD+, a fluorocarbon surfactant, BSA (bovine serum albumin), sucrose, trehalose, mannitol and a preservative; and the reagent R2 is composed of a buffer solution, Mg<2+>, BSA, sucrose, trehalose, mannitol, glucose-6-phosphate dehydrogenase, hexokinase, a fluorocarbon surfactant and a preservative. The reagent has the advantages of high accuracy, high stability and low price, is convenient to use and can completely satisfy the clinical demands.

Description

technical field [0001] The invention relates to the technical field of glucose detection, in particular to a serum glucose detection reagent. Background technique [0002] Serum glucose (GLU), also known as blood sugar, refers to the concentration of glucose in the blood. In the human body, it mainly comes from the glucose produced by digestion and absorption of starch, muscle glycogen and maltose in food. Most of the blood sugar is stored in the liver and muscles in the form of glycogen, and a small part is transported to tissues and organs along with the blood circulation, where it is oxidized and decomposed to provide energy for life activities. [0003] Under normal circumstances, with the participation of insulin and other hormones, the decomposition and synthesis of serum glucose are in a dynamic balance, and blood sugar remains relatively stable. The fasting blood glucose concentration of normal people is 3.61~6.11mmol / L. A fasting blood glucose concentration excee...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/48
Inventor 郁东李志明谭柏清甘宜梧谢清华
Owner 郁东
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