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Serum creatine kinase detection reagent

A serum creatine kinase and detection reagent technology, which is applied in the field of serum creatine kinase detection reagents, can solve problems affecting the accuracy of detection results and the application of reagents, and achieve improved accuracy and stability, reduced impact, and anti-interference strong effect

Active Publication Date: 2015-02-25
BIOBASE BIODUSTRY (SHANDONG) CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In conventional reagent testing, the presence of pyruvate, heparin and other interfering substances in the sample often affects the accuracy of the test results, which in turn affects the application of the reagents in the market.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0067] A serum creatine kinase detection reagent is composed of reagent R1 and reagent R2 according to the volume ratio of 4:1;

[0068] Reagent R1 is composed of the following ingredients:

[0069] Imidazole buffer (pH=6.5) 50 mmol1

[0070] Glucose 50mmo1

[0071] Nanoparticles 50mmol / L

[0072] N-acetylcysteine ​​20mmo1

[0073] Disodium edetate 1mmo1

[0074] Adenosine diphosphate 5mmo1

[0075] Coenzyme Ⅰ 10mmo1

[0076] Adenine ribonucleotide 10mmo1

[0077] Pyruvate decarboxylase 0.5KU

[0078] Glucose 6-Phosphate Dehydrogenase 5KU

[0079] Hexokinase 2KU

[0080] Sodium dodecylbenzenesulfonate 1g

[0081] Add deionized water to 1L;

[0082] Reagent R2 is composed of the following ingredients:

[0083] Imidazole buffer (pH=7.5) 100mmo1

[0084] Phosphocreatine 100mmo1

[0085] Sodium azide 1g

[0086] Sodium dodecylbenzenesulfonate 20g

[0087] Make up to 1 L with de...

Embodiment 2

[0094] A serum creatine kinase detection reagent is composed of reagent R1 and reagent R2 according to the volume ratio of 4:1;

[0095] Reagent R1 is composed of the following ingredients:

[0096] Imidazole buffer (pH=7.5) 70mmo1

[0097] Glucose 30mmo1

[0098] Nanoparticles 30mmol / L

[0099] N-acetylcysteine ​​25mmo1

[0100] Disodium EDTA 6mmo1

[0101] Adenosine diphosphate 10mmo1

[0102] Coenzyme Ⅰ 5mmo1

[0103] Adenine ribonucleotide 20mmo1

[0104] Pyruvate decarboxylase 1.2KU

[0105] Glucose 6-Phosphate Dehydrogenase 4KU

[0106] Hexokinase 3KU

[0107] Sodium dodecylbenzenesulfonate 10g

[0108] Add deionized water to 1L;

[0109] Reagent R2 is composed of the following ingredients:

[0110] Imidazole buffer (pH=6.5) 80mmo1

[0111] Phosphocreatine 150mmo1

[0112] Proclin300 5g

[0113] Sodium dodecylbenzenesulfonate 10g

[0114] Make up to 1 L with deionized...

Embodiment 3

[0118] A serum creatine kinase detection reagent is composed of reagent R1 and reagent R2 according to the volume ratio of 4:1;

[0119] Reagent R1 is composed of the following ingredients:

[0120] Imidazole buffer (pH=7.0) 100 mmol1

[0121] Glucose 20mmo1

[0122]Nanoparticles 10mmol / L

[0123] N-acetylcysteine ​​30mmo1

[0124] Disodium edetate 10mmo1

[0125] Adenosine diphosphate 1mmo1

[0126] Coenzyme Ⅰ 1mmo1

[0127] Adenine ribonucleotide 30mmo1

[0128] Pyruvate decarboxylase 2KU

[0129] Glucose 6-phosphate dehydrogenase 2KU

[0130] Hexokinase 5KU

[0131] Sodium dodecylbenzenesulfonate 20g

[0132] Add deionized water to 1L;

[0133] Reagent R2 is composed of the following ingredients:

[0134] Imidazole buffer (pH=7.0) 50mmo1

[0135] Phosphocreatine 200mmo1

[0136] Sodium azide 5g

[0137] Proclin300 5g

[0138] Sodium dodecylbenzenesulfonate 1g

[0139] M...

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Abstract

The invention discloses a creatine jubase detection reagent. The reagent disclosed by the invention consists of a reagent R1 and a reagent R2 according to a volume ratio being 4 to 1, wherein the reagent R1 consists of an imidazole buffer solution, glucose, nano particles, N-acetylcysteine, ethylenediaminetetraacetic acid disodium salt, adenosine diphosphate, coenzyme I, adenine ribonucleotide, pyruvate decarboxylase, 6-phosphogluconic dehydrogenase, hexokinase and sodium dodecyl benzene sulphonate; and the reagent R2 consists of an imidazole buffer solution, phosphocreatine, a preservative and sodium dodecyl benzene sulphonate. Pyruvate decarboxylase and gamma-Fe2O3 nano particles are added into the creatine jubase detection reagent disclosed by the invention, so that interfering substances in a serum sample can be effectively eliminated, metal ions in the serum sample are chelated by matching sodium dodecyl benzene sulphonate and ethylenediaminetetraacetic acid disodium salt, the influence on reaction enzymes is alleviated, and the reagent is a stable, accurate and sensitive detection reagent with high interference resistance.

Description

technical field [0001] The invention relates to a biological reagent, in particular to a detection reagent, in particular to a serum creatine kinase detection reagent. Background technique [0002] Creatine Kinase (Creatine Kinase, CK) (ATP: Creatine N-phosphotransferase EC 2.7.3.2) is also known as phosphocreatine kinase (CPK). CK mainly exists in skeletal muscle, brain and cardiac muscle. Creatine kinase has a high value in the diagnosis of acute myocardial infarction. It is usually found in the cytoplasm and mitochondria of animal heart, muscle and brain tissues. It is an important kinase directly related to intracellular energy operation, muscle contraction, and adenosine triphosphate (ATP) regeneration. It reversibly Catalyzes the transphosphoryl reaction between creatine and ATP. Increased CK activity: mainly seen in myocardial infarction and various types of progressive muscular atrophy, skeletal muscle injury, muscular dystrophy, acute myocarditis, cerebrovascular...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/52G01N21/31
CPCC12Q1/32C12Q1/485C12Q1/50C12Q1/527G01N2333/9123
Inventor 谢清华王进甘宜梧谭柏清李静赵新朱玉娥
Owner BIOBASE BIODUSTRY (SHANDONG) CO LTD
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