Glucose detection reagent being strong in stability and low in cost and adopting hexokinase

Abstract

The invention relates to the technical field of detection for GLU in the blood by adopting hexokinase, and in particular relates to a detection reagent for GLU in the blood, which is strong in stability and low in cost and adopts liquid hexokinase. The detection reagent adopts liquid double reagents, wherein the reagent I mainly contains a buffer solution, Mg<2+>, an ion balancing agent, a preservative, NAD (oxidized coenzyme I), a protective agent and a surfactant, and the reagent II contains a buffer solution, a protective agent, a surfactant, preservative, hexokinase (HK), glucose-6-phosphate dehydrogenase (G6P-DH), ATP.NA2, and the like. In order to guarantee the stability of enzymes in the reagent, various protective agents including polyethylene glycol-20000, tween-80, FAD and the like are added in the reagent in a pertinence manner, in order to reduce the cost, especially the use of enzymes, various surfactants are added in the reagent, so that the emulsifying for enzymes is enhanced, the service efficiency for enzymes is improved, thus the cost of the reagent is greatly reduced, and the detection reagent is particularly suitable for being used and popularized in clinic.

Description

technical field [0001] The invention relates to a highly stable hexokinase method detection reagent, which belongs to the technical field of clinical in vitro detection. Background technique [0002] The sugar in the serum is called blood sugar, and in most cases it is glucose. Most of the energy required for the activities of various tissue cells in the body comes from glucose, so blood sugar must be maintained at a certain level to maintain the needs of various organs and tissues in the body. normal people in the morning fasting blood sugar The concentration is 80-120 mg%. A fasting blood glucose concentration greater than 130 mg% is called high blood sugar . If the blood sugar concentration exceeds 160-180 mg%, part of the glucose will be excreted with the urine, which is diabetes. A blood sugar concentration below 70 mg% is called Hypoglycemia . Glucose exists in the plasma and lymph of the human body, and is an indispensable substance in life activities. It can...

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Problems solved by technology

[0003] There are many detection methods for serum glucose, the detection methods mainly include oxidase coupling, colorimetry, microcurrent method, oxygen rate method, hexokinase method, o-toluidine method, glucose dehydrogenase method, gas chromatography, chromatography, isotope dilution mass spectrometry Among them, the most commonly used methods in medicine are the oxidase method and the hexokinase method. Most of the other methods have poor accuracy, high cost, complicated operation, and high requirements for instruments and operators. The actual use is less, but now the oxidase method is used more, because uric acid, vitamin C, bilirubin, etc. can compete with chromogenic substances for hydrogen peroxide and inhibit the reaction during the detection process, resulting in low results, and The linear range of this method is narrow, which brings great changes to the clinical application. The hexokinase method for the determination of glucose in serum is suitable for patients with mild hemolysis, lipemia, jaundice, vitamin C, sodium fluoride, heparin, etc. , EDTA and oxalate are not easy to interfere with the determination of this method, and are suitable for automatic analyzers. It is an internationally recognized reference method and can very well make up for the defects of the oxidase method in the detection of blood glucose. However, this detection method involves Raw materials such as glucokinase (HK), glucose-6-phosphate dehydrogenase (G6P-DH) and ATP, most of these raw materials need to be stored in the state of dry powder at -20°C, and once configured into reagents, they are easy to decay. Existing Some of the products are mostly made into dry powder or increase the content of the main components in the reagent to slow down the attenuation of the main components in the liquid reagent. The dry powder reagent needs to be reconstituted before use, which brings inconvenience to the operation, and once reconstituted It needs to be used up as soon as possible, otherwise it will fail in a short period of time, and increasing the amount of main raw materials will greatly increase the cost, but it cannot fundamentally protect the active ingredients in the reagent. Therefore, a stable and low-cost liquid hexose Kinase method to detect serum glucose detection reagent has great prospects

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