The invention provides a preparation method and application of total flavone and total
polysaccharide in
cyclocarya paliurus leaves. The method comprises the steps of taking
cyclocarya paliurus leavesas raw materials, executing extraction by an
ethanol-water
solvent, executing extraction by an
organic solvent, executing
elution by a
chromatographic column and by
macroporous resin to obtain totalflavone, conducting extraction on
cyclocarya paliurus leaf dregs by hot water, executing
precipitation, removing
protein, and adsorbing impurities by
macroporous resin to obtain total
polysaccharide.Studies show that the total triterpenes, the total
flavones, the total polysaccharides and their composition prepared by the method can effectively increase
glucose uptake of 3T3-L1 adipocytes, obviously reduce the
fasting blood glucose level of the hereditary diabetes db / db mice, has antidiabetic activity, and can be used for preparing products such as antidiabetic medicines, health foods, functional foods and the like for reducing
blood sugar. The invention develops the comprehensive preparation method of total
flavones and total polysaccharides in cyclocarya paliurus leaves and applicationof the mehtod in resisting diabetes, and provides a basis and a new application direction for popularization, utilization and
deep processing of traditional Chinese medicinal materials and new food raw materials.