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Collagen zymolyte with blood sugar reducing effect as well as preparation method and application of collagen zymolyte

A collagen and enzymatic hydrolyzate technology, applied in the field of collagen protease hydrolyzate and its preparation, can solve problems such as no breakthrough, and achieve the effects of realizing enzymatic hydrolysis controlled release, simple process, and improving oral glucose tolerance.

Active Publication Date: 2022-04-15
SOUTH CHINA UNIV OF TECH +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, it has been reported that certain peptides have relatively good DPP-IV inhibitory activity, but how to release these DPP-IV inhibitory peptides from collagen to prepare collagen hydrolyzate with strong DPP-IV inhibitory activity, It is still a technical difficulty and has not yet been broken through

Method used

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  • Collagen zymolyte with blood sugar reducing effect as well as preparation method and application of collagen zymolyte
  • Collagen zymolyte with blood sugar reducing effect as well as preparation method and application of collagen zymolyte
  • Collagen zymolyte with blood sugar reducing effect as well as preparation method and application of collagen zymolyte

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0050] The preparation method of fish skin hydrolyzate comprises the following steps:

[0051] (1) Thaw 1 kg of fish skin (tilapia fish skin, the same below), drain the water, then add 6L of 0.05% sodium hydroxide solution, soak for 4 hours, wash with running water until neutral, and obtain alkali-treated fish. Skin;

[0052] (2) adding alkali-treated fish skin to 10 L of hot water at a temperature of 90° C., and heat-treating for 5 hours to obtain a heat-treated fish skin solution;

[0053] (3) Cool the heat-treated fish skin solution to 37° C., adjust the pH value of the solution to 8.0, add 1 g of trypsin to it, enzymatically hydrolyze it for 8 hours, and then inactivate the enzyme at 95° C. for 30 minutes to obtain a solution after enzymatic hydrolysis;

[0054](4) Cool the solution after the first enzymolysis to 50°C, adjust the pH value to 7.0, add 1g bromelain and 1g neutral protease to it, and after enzymolysis for 6h, inactivate the enzyme at 95°C for 30min to obtain...

Embodiment 2

[0058] The preparation method of fish skin hydrolyzate comprises the following steps:

[0059] (1) Thaw 1.4 kg of fish skin, drain the water, then add 7 L of 2% sodium hydroxide solution, soak for 2 hours, wash with running water until neutral, and obtain alkali-treated fish skin;

[0060] (2) adding the alkali-treated fish skin to 21 L of hot water at a temperature of 95° C., and heat-treating for 4 hours to obtain a heat-treated fish skin solution;

[0061] (3) Cool the heat-treated fish skin solution to 50°C, adjust the pH value of the solution to 7.0, add 1.2g of flavor protease to it, and after enzymolysis for 6 hours, inactivate the enzyme at 95°C for 30 minutes to obtain a solution after enzymolysis ;

[0062] (4) Cool the solution after the first enzymolysis to 50°C, adjust the pH value to 7.0, add 1.5g of neutral protease and 1.5g of papain to it, and after enzymolysis for 6h, inactivate the enzyme at 95°C for 30min to obtain For the solution after the second enzyma...

Embodiment 3

[0066] The preparation method of fish skin hydrolyzate comprises the following steps:

[0067] (1) Thaw 0.6 kg of fish skin, drain the water, then add 4 L of 1.5% sodium hydroxide solution, soak for 2.5 hours, wash with running water until neutral, and obtain alkali-treated fish skin;

[0068] (2) adding the alkali-treated fish skin to 5 L of hot water at a temperature of 85° C., and heat-treating it for 6 hours to obtain a fish skin solution after heat treatment;

[0069] (3) Cool the heat-treated fish skin solution to 37° C., adjust the pH value of the solution to 8.0, add 2 g of trypsin to it, enzymatically hydrolyze it for 4 hours, and inactivate the enzyme at 95° C. for 30 minutes to obtain a solution after enzymatic hydrolysis;

[0070] (4) Cool the solution after the first enzymolysis to 50°C, adjust the pH value to 7.0, add 1.5g bromelain and 1g papain to it, and after enzymolysis for 10 hours, inactivate the enzyme at 95°C for 30 minutes to obtain the second The solu...

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Abstract

The invention discloses a collagen zymolyte with a blood sugar reducing effect and a preparation method and application thereof, the zymolyte contains Gly-Pro-type peptide composed of 4-7 amino acid residues, and the mass ratio is 30-45%; the general formula of the sequence of the Gly-Pro-type peptide consisting of 4 to 7 amino acid residues is Gly-Pro-Xaa1-Gly-[Xaa2] r-[Xaa3] s-[Gly] t. According to the preparation method provided by the invention, the zymolyte with strong DPP-IV inhibition activity is prepared for the first time, and the DPP-IV inhibition rate of the zymolyte is as high as 68% when the final concentration is 1mg / mL; the zymolyte is rich in Gly-Pro-type peptide composed of 4-7 amino acid residues, and the peptide yield is as high as 45%; animal experiments prove that the zymolyte can regulate fasting blood-glucose of rats with type 2 diabetes mellitus and improve the oral glucose tolerance of the rats with type 2 diabetes mellitus.

Description

technical field [0001] The invention relates to a biologically active peptide for type 2 diabetes, in particular to a collagen hydrolyzate with hypoglycemic effect and its preparation method and application. Background technique [0002] Dipeptidyl peptidase-IV (DPP-IV) is an important target for the treatment of type 2 diabetes. At present, the DPP-IV inhibitors that have been approved for clinical use mainly include sitagliptin and saxagliptin. Although these drugs can effectively regulate the blood sugar level of diabetic patients, they will more or less cause some side effects, such as nausea, itching and nasopharyngitis, etc., and there has always been controversy about whether long-term use will cause serious side effects. Therefore, if the functional substance with DPP-IV inhibitory activity can be obtained from natural food, it will bring benefits to human health. [0003] At present, it has been reported that certain peptides have relatively good DPP-IV inhibitory...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K14/78C07K5/103C12P21/06A61K38/07A61K38/08A61K38/01A61K38/39A61P3/10
Inventor 郑淋徐琼瑶赵谋明赵翊君
Owner SOUTH CHINA UNIV OF TECH
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