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Bacterial strain as well as composition and application

A composition and strain technology, applied in the direction of drug combination, bacterial antigen components, drug delivery, etc., can solve problems such as adverse reactions of patients and drugs that cannot achieve the expected effect

Active Publication Date: 2021-07-06
MOON (GUANGZHOU) BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, many weight loss drugs are restricted from the market or withdrawn from clinical use because some of them do not achieve the desired effect or because they cause serious adverse reactions in patients

Method used

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  • Bacterial strain as well as composition and application
  • Bacterial strain as well as composition and application
  • Bacterial strain as well as composition and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0107] This example provides the isolation and identification of Christensenella sp. MNO-863.

[0108] (1) Isolation of MNO-863

[0109] Christensenella sp.MNO-863 of the present invention is isolated from a stool sample of a healthy male volunteer of Han nationality in Guangzhou City, Guangdong Province. The volunteer did not use antibiotics in the previous three months when the sample was collected.

[0110] Dispense normal saline into sterile 10ml centrifuge tubes in a biological safety cabinet; transfer the anaerobic blood plate (Jiangmen Kailin anaerobic blood agar medium, Guangdong Mechanical Note 20172400940) and sterile normal saline into an anaerobic tube 24 hours in advance. In the oxygen workbench, pour 5-7 sterile glass beads into the solidified anaerobic blood plate.

[0111] Take an appropriate amount of fresh stool samples from volunteers and store them in a sample containing sterile preservation solution (3% PEG solution, that is, weigh 30g of polyethylene gly...

Embodiment 2

[0135] In this example, an in vivo test of MNO-863 in a high-fat diet-induced obese mouse model was carried out to verify its use in the treatment or prevention of liver function damage and related diseases.

[0136] Experimental Materials:

[0137] (1) Experimental animals: 32 C57BL / 6J male mice (purchased from Jiangsu Jicui Yaokang Biotechnology Co., Ltd.) were purchased, which were normally fed mice and were 5 weeks old. The mice were kept in the same environment during the growth process, and 8 of them were given SPF-grade rat maintenance feed (Guangzhou Hancheng Experimental Equipment Co., Ltd.), and the remaining 24 were fed with D12492 high-fat feed (Pike Biological) for about 8 to 10 weeks. Weighing, diet-induced obesity model modeling standard is that the body weight reaches 38.00±2.00g.

[0138] (2) Tested bacterial strain: MNO-863 of anaerobic culture, culture medium is 104 liquid culture medium in embodiment 1, cultivates 48h under 37 ℃ of anaerobic conditions, to...

Embodiment 3

[0165] In this example, an in vivo test of MNO-863 in an obese mouse model induced by a high-fat diet was carried out to verify its use in treating or preventing diabetes. Experimental Materials:

[0166] (1) Experimental animals: 40 C57BL / 6J male mice (purchased from Jiangsu Jicui Yaokang Biotechnology Co., Ltd.) were purchased, which were normally reared mice and were 5 weeks old. The mice were kept in the same environment during the growth process. Eight of them were given SPF-grade rat maintenance feed (Guangzhou Hancheng Experimental Equipment Co., Ltd.), and 32 were given D12492 high-fat feed (Pike Bio) for about 8 to 10 weeks, and then weighed. , Diet-induced obesity model modeling standard is to reach a body weight of 38.00±2.00g.

[0167] (2) Tested strain: MNO-863 cultured in anaerobic mode, the medium is 104 liquid medium, cultivated under anaerobic conditions at 37°C for 48 hours, until the bacterial concentration is about 10 11 The order of CFU / mL can be used as...

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Abstract

The invention discloses a bacterial strain as well as a composition and application, and relates to the technical field of strain separation and application. The Cristinia sp. provided by the invention can be applied to treatment or prevention of liver function impairment and liver function impairment related diseases, alimentary canal mucosal injury and alimentary canal mucosal injury related diseases, diabetes, obesity and obesity related diseases. The inventor verifies that the Cristinia sp. provided by the invention has no toxic or side effect on the kidney and can reduce the weight of the liver; the initial steatohepatitis focus can be treated; fat accumulation of liver cells can be slowed down; AST and ALT in serum can be reduced; and abdominal white fat inflammatory lesion can be reduced. The Cristinia sp. can also repair the digestive tract mucosa, recover the barrier function of the mucosa, and prevent and treat intestinal leakage, peptic ulcer and other diseases caused by barrier function impairment. The Cristinia sp. also has the effects of reducing fasting blood-glucose of a body, regulating insulin level and reducing body fat of mammals, and has the effects of preventing and treating diabetes and improving the metabolic function of obese patients.

Description

technical field [0001] The invention relates to the technical field of bacterial strain isolation and application, in particular to a bacterial strain, composition and use. Background technique [0002] At present, the prevention and treatment of liver disease are mainly through hepatitis vaccination, reducing alcohol consumption, improving dietary structure and physical exercise. However, these strategies often can only play a preventive role, and the benefits are very small. The effect varies with individual constitutions. Once faced with the liver disease that has occurred, it seems helpless. Therefore, there is an urgent need for countermeasures that can effectively prevent and treat liver diseases, and it is necessary to develop an effective method or drug that can prevent and treat liver diseases with low side effects. [0003] Gastrointestinal mucosal injury, especially intestinal mucosal injury will not only affect the digestion and absorption of nutrients, but also...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K35/741A61K9/19A61K39/02A61P1/16A61P1/04A61P3/10A61P3/04A61P3/06A61P1/00A61P9/00A61P5/50
CPCA61K35/741A61K9/19A61K9/0053A61K9/0019A61K39/0208A61P1/16A61P1/04A61P3/10A61P3/04A61P3/06A61P1/00A61P9/00A61P5/50
Inventor 林诠盛蒋先芝邝祖鹏黄宝家孔萍邓茜莹赵莹莹肖晨张腾勋邝茜雯泰利宏
Owner MOON (GUANGZHOU) BIOTECH CO LTD
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