Sweet cherry rootstock Colt tissue culture method

A technology of tissue culture and sweet cherry, applied in the field of plant tissue culture, can solve the problems of low multiplication coefficient of Colt tissue culture seedlings, difficulty in rooting, inability to realize large-scale industrial production, etc.

Inactive Publication Date: 2013-06-19
天水市果树研究所
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In the prior art, the tissue culture seedlings of sweet cherry rootstock Colt have a low multiplication coefficient, d

Method used

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  • Sweet cherry rootstock Colt tissue culture method
  • Sweet cherry rootstock Colt tissue culture method
  • Sweet cherry rootstock Colt tissue culture method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] A kind of tissue culture rapid breeding seedling method of sweet cherry stock Colt, it comprises the following steps:

[0038] (1) Handling of raw materials

[0039] Before germination in spring, select Colt annual branches with strong growth and full buds, cut them into 2 cm bud stems in the laboratory, wash them with 1000 times hallipin solution for 10-15 minutes, then rinse them in running water for 10 minutes, and then put into a 150ml triangular flask, and add newly configured 0.1% HgCL on the ultra-clean workbench 2 The solution was sterilized for 8 minutes, and finally soaked and washed with sterile water for 4 times, about 5 minutes each time. In the ultra-clean work, use a sterilized scalpel to peel off the phosphorus sheet of the overwintering buds, and quickly cut off the shoot tip, which is used as an explant for later use.

[0040] (2) Primary culture

[0041] The explants were inoculated and cultured on the primary medium, which was made by adding the fo...

Embodiment 2

[0057] A kind of tissue culture rapid breeding seedling method of sweet cherry stock Colt, it comprises the following steps:

[0058] (1) Handling of raw materials

[0059] Processing of raw materials: before spring germination, select Colt annual branches with strong growth and full buds, cut them into 2 cm bud stems in the laboratory, wash them with 1000 times hallipin solution for 10-15 minutes, and then rinse them in running water for 10 minutes. Minutes, then put it into a 150ml Erlenmeyer flask, and add the newly configured 0.1% HgCl on the ultra-clean workbench 2 Disinfect the solution for 8 minutes, and finally soak and wash it with sterile water for 4 times, about 5 minutes each time, peel off the phosphorus sheet of the overwintering bud with a sterile scalpel on the ultra-clean work, quickly cut the stem tip, and use it as an explant for later use .

[0060] (2) Primary culture

[0061] The explants were inoculated and cultured on the primary medium, which was ma...

Embodiment 3

[0077] A kind of tissue culture rapid breeding seedling method of sweet cherry stock Colt, it comprises the following steps:

[0078] (1) Handling of raw materials

[0079] Before germination in sweet spring, select Colt annual branches with strong growth and full buds, cut them into 2 cm bud stems in the laboratory, wash them with 1000 times hallipin solution for 10-15 minutes, then rinse them in running water for 10 minutes, and then Put it into a 150ml triangular flask, and add 0.1% HgCL of the new configuration on the ultra-clean workbench 2 Disinfect the solution for 8 minutes, and finally soak and wash it with sterile water for 4 times, about 5 minutes each time, peel off the phosphorus sheet of the overwintering bud with a sterile scalpel on the ultra-clean work, quickly cut the stem tip, and use it as an explant for later use .

[0080] (2) Primary culture

[0081] The explants were inoculated and cultured on the primary medium, which was made by adding the followin...

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Abstract

The invention discloses a sweet cherry rootstock Colt tissue culture method. The method comprises the following steps that (1) an annual sweet cherry rootstock Colt is selected for primary culture, an overwintering bud phosphorus slice of the annual sweet cherry rootstock Colt is striped off to cut and take a stem apex to be inoculated on a primary culture medium for culturing, the culture medium is made by the fact that raw materials are added into a 1/2 MS, and the raw materials and proportions of the raw materials are white granulated sugar of 25g, agar powder of 5g, 6-benayl aminopurine of 0.5-2.0mg, and indolebutyric acid of 0.01-0.10mg; (2) subculturing is transferred into an enrichment medium to be cultured, the enrichment medium is made by the fact that raw materials are added into a 1/2 MS enrichment medium of one liter, and the raw materials and proportions of the raw materials are white granulated sugar of 25g, agar powder of 5g, 6-benayl aminopurine 1.0-1.5mg, and indolebutyric acid of 0.15-0.20mg; (3) rooting culture is transferred into a rooting medium to be cultured, the rooting medium is made by the fact that raw materials are added into a 1/2 MS minimal medium, and the raw materials and proportions of the raw materials are white granulated sugar of 25g, agar powder of 5g, indolebutyric acid of 0.25-0.75mg; and (4) hardening-seedling and transplantation are carried out.

Description

technical field [0001] The invention belongs to the technical field of plant tissue culture, in particular to a method for propagating sweet cherry rootstock Colt through plant tissue culture technology. Background technique [0002] The sweet cherry rootstock Colt was introduced from the East Maolin Experimental Station in the United Kingdom. It was bred by crossing Mazard and Chinese cherry. It is an excellent cherry rootstock in the United Kingdom. It is suitable for high-yield cultivation in large-scale cherry orchards. Good, the plant has many branches, grows in a cluster, has strong grafting affinity, the seedlings grow robustly, the planting and gardening phase is neat, the plant growth and results are good, and it has good yield and stability. It grows by grafting with different cherry varieties According to the survey, each variety shows good affinity and stable characters. It is resistant to diseases in cultivation management, vigorous tree growth, early fruit and ...

Claims

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Application Information

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IPC IPC(8): A01H4/00
Inventor 赵新红周代琴李帼英杨映红杨俊霞王艳芳郭志刚杨瑞斌王花李海青马建芳
Owner 天水市果树研究所
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