109results about How to "Good rooting effect" patented technology

The invention relates to a bio-organic compound fertilizer, which comprises the following components in part by weight: 10 to 30 parts of organic matter, 2 to 10 parts of compound microbial inoculants, 5 to 20 parts of humic acid, 5 to 20 parts of amino acid, 4 to 10 parts of chitin, 1 to 5 parts of polypeptide, 530 parts of nitrogen, phosphorus and potassium, 5 to 10 parts of secondary and trace elements, 5 to 15 parts of algae fertilizer, 5 to 10 parts of zeolite powder and 1 to 5 parts of synergistic agent. Through the scientific and rational formula, the bio-organic compound fertilizer achieves nutritive equilibrium and can satisfy the need of crop growth in nutrient. The microbial inoculants are added to quickly degrade natural macromolecular organic compounds into the organic fertilizer and decompose organic pollutants, which effectively improves the capacity of crops in the absorption of nutrient components, reduces the waste of the fertilizer and reduces the damage of the chemical fertilizer to soil to further achieve the nutritive equilibrium, satisfy the need of the crop growth in the nutrient, achieve the aims of low investment and high output value, improve the quality of the crops and improve the economic benefit brought by the crops.

The invention relates to an improved American red maple twig cutting propagation method and belongs to the technical field of plant cultivation. The improved American red maple twig cutting propagation method comprises the steps that (1) a disinfected hot bed is set up on a selected breeding ground, arc frames are arranged on a seedbed, and disinfected cutting mediums are laid in the seedbed; (2) a half-lignified twig is cut from an improved American red maple maternal plant, and the twig is cut into a cutting slip with a length of 8-10 cm and with two buds and two pieces of half leaves; (3) the lower incision of the cutting slip is soaked in rooting agent, rooting powder solution, and hormone solution to facilitate rooting; (4) the cutting slip is inserted in the seedbed mediums, the depth of insertion is 2-4 cm, the density is that the space in the rows is 5cm and the space between rows is 6 cm, and the leaves face the same direction; and (5) the mediums are watered thoroughly after the cutting slip is implanted in, then a plastic thin film and a shading net are covered on the arc frames, and management after implanting begins. According to the improved American red maple twig cutting propagation method, the operation is simple without restrictions of regions and weather conditions, the speed of reproduction is fast, rate of survival is more than 95%, an improved American red maple seedling obtained is developed in root system and good in quality, and thus market prospects are broad.

The present invention relates to a high-effective water soluble rooting powder for Mandiya taxus, belonging to a fast propagation technique for forest tree seed seedling. It uses alpha-naphthylacetic acid and sodium salt or potassium salt powder of indolylbuteric acid as main component, mixes them according to the ratio of 1:0.6-1.5 to obtain the invented rooting powder. In said powder material a proper quantity of rare earth microfertilizer also can be added. Besides, said invention also provides the application method and concrete steps of said rooting powder.

The invention discloses a method for cultivating, rapidly propagating and regenerating tissues of seedlings of radix aconiti lateralis preparata. Stem fragments with auxiliary buds, stem fragments, sterile leaves and seeds of the radix aconiti lateralis preparata are used as explants, rapid propagation systems and seed sterile cultivation and regeneration systems for optimizing the radix aconiti lateralis preparata are built by the aid of links of disinfection conditions, bud induction, bud multiplication, rooting and transplanting, callus induction, multiplication and differentiation and the like, and technical support can be provided for industrializing seedling growing. The method includes a process for rapidly propagating the radix aconiti lateralis preparata, a process for regenerating and propagating the radix aconiti lateralis preparata and a process for rapidly growing the seedlings of the radix aconiti lateralis preparata. The process for rapidly propagating the radix aconiti lateralis preparata includes a technology for rapidly propagating the stem fragments of the radix aconiti lateralis preparata and a technology for rapidly propagating the seeds; the process for regenerating and propagating the radix aconiti lateralis preparata includes a technology for regenerating and propagating the stem fragments and adventitious roots of the radix aconiti lateralis preparata and a technology for regenerating and propagating the sterile leaves of the radix aconiti lateralis preparata. The stem fragments and the adventitious roots of the radix aconiti lateralis preparata are used as explants in the technology for regenerating and propagating the stem fragments and adventitious roots of the radix aconiti lateralis preparata. The sterile leaves of the radix aconiti lateralis preparata are used as explants in the technology for regenerating and propagating the sterile leaves of the radix aconiti lateralis preparata. The method has the advantages that the radix aconiti lateralis preparata seedling rapid propagation and growing efficiency can be obviously improved, the seedling growing periods can be obviously shortened, technical support can be provided for production application of radix aconiti lateralis preparata seedling rapid propagation and growing, and problems in the aspect of seedling propagation and growing on current markets can be effectively solved.

The invention discloses a hardwood cutting reproduction method for tetraploid robinia pseudoacacia, and belongs to the field of landscape plants. The method comprises the following steps of: taking annual hardwood cuttings of the tetraploid robinia pseudoacacia as a material; sterilizing hardwood cuttings of the tetraploid robinia pseudoacacia and treating by using a plant growth regulator; and heating cutting soil cooperatively. The method specifically comprises the following steps of: treating cuttings, namely immersing the base parts of the cuttings into carbendazim with concentration of 5,000 mg / L for 3 minutes and immersing the cuttings with heteroauxing solution with concentration of 1,000 mg / L; and finally heating the cutting soil, namely after hardwood cutting of spring tetraploid robinia pseudoacacia, monitoring heated cutting soil by using a TNHY-4 soil temperature monitor, and controlling the cutting soil at depth of 7-9 cm of a heating seed bed at 25-27 DEG C. Through the hardwood cuttings reproduced by the tetraploid robinia pseudoacacia by using the method, the rooting rate of the method is obviously improved, the rooting progress is quickened, and the technical problem of hardwood cutting seedling raising of the tetraploid robinia pseudoacacia in cold weather at northern areas is solved.

The invention relates to a method for cutting and seedling a hybrid hazel small and tender etiolated tiller seedling. The method comprises the following steps of: selecting a cutting, preparing before cutting, cutting, constructing a tunnel, managing after cutting, outplanting a cutting seedling and the like. The method takes a large-fruit, high-yield and high-cold resistance hybrid hazel as a material, fully utilizes the characteristics that the tree has high sprouting capacity, high activity of small and tender etiolated cell tissues and susceptibility to outside stimuli to form an adventitious root, adopts simple greenhouse cutting propagation, improves coppice shoot utilization efficiency and propagation rate of merchandised nursery stock in a unit time, makes up a current situation of the shortage of hybrid hazel seedlings in production, and promotes scale development in Xinjiang and other areas suitable for planting hybrid hazel. Compared with the prior propagation art of the hybrid hazel, the method fully utilizes small and tender coppice shoot, and has the characteristics of simple, practicable and easily-grasped method, low seedling propagation cost, quick response, good rooting effect, and high practicability and operability.

The invention discloses a peach twig cutting propagation method. The method comprises the following specific steps: selection of cuttings, pruning of the cuttings, establishment of a seedbed, preparation before cuttage, cuttage and management and transplantation. According to the peach twig cutting propagation method, the propagation speed is high, the survival rate is high, the method is simple and easy to operate, the rooting effect is good, seedlings grow vigorously and do not require a seedling recovery period after transplantation, the survival rate is up to 95.0% or higher, the seedling raising time is short, the seedlings can be transplanted within 2 months, large-scale seedling production can be realized, and the method has relatively high ecological and economic benefits.

The invention relates to jujube tree branch cuttage reproduction pretreatment liquid. The jujube tree branch cuttage reproduction pretreatment liquid consists of the following raw materials in parts by weight: alpha-naphthylacetic acid, indoleacetic acid, rooting power, wicker leaching agent, washing wastewater, sodium nitrophenolate, p-chlorophenoxyacetic acid, a potassium permanganate solution, copper hydroxide, deltamethrin and ammonium phosphate. The pretreatment liquid has remarkable effects on the aspects of branch differentiation and rooting, root hair growth, sterilization and deinsectization as well as nutrition supply. On the basis of scientific experiments, the raw material proportion and dosage are controlled, so that the cuttage reproduction survive rate can be effectively increased.

The invention discloses a pomegranate old branch cuttage and rapid propagation method. According to the method, pomegranate tress of 25-35 years are selected, old branches,15-25 years old, on the pomegranate tress are collected to serve as cuttings, the cuttings are soaked with clean water and then soaked with a special-effect rooting powder solution, and the cuttings are placed into grass carbon in a cuttage mode in late March every year; sand is mixed into a medium according to the volume ratio of 1-1.5:2-3, a mulching film covers the surface of the mixture, cuts in the cuttings are exposed out of the mulching film and are wrapped for moisture retention, and a seedling management stage starts. The rooting rate of pomegranate old branch cuttings propagated in the method is as high as 95%, and the method is easy to operate, short in period, low in cost and quick in effect, solves the technical problems of pomegranate old branch cuttage and propagation and lays a foundation for manufacturing thick, strong and unadorned Taoist potted landscapes.

The invention discloses a novel peony tissue culture rooting method. Buds of pea green, island kam and yellow peonies are used as explants; under a germfree condition, the buds are disinfected by a three-step disinfection method; tissue culture rooting seedlings are obtained by three steps of constructing a germfree strain, performing subculture multiplication culture and performing root induction culture; and the rooting rates are respectively that the pea green peony is 50.0-66.7 percent, the island kam peony is 33.3-86.7 percent and the yellow peony is 31.4-73.5 percent.

The invention discloses a method for shoot cuttage rejuvenation of superior adult trees of pinus khasys. The method includes grafting rejuvenation of branches of the superior adult trees of the pinus khasys, according to the first time of grafting rejuvenation, lignification lateral branches, which are grown in the current year, of the superior adult trees of the pinus khasys are cut to serve as scions to be grafted on seedling stocks which are grown for one year through clone seeds; according to the second time of grafting rejuvenation, lignification lateral branches grown on seedlings in the first time of grafting rejuvenation are cut to serve as scions to be grafted on stocks which are newly selected; according to the third time of grafting rejuvenation, lignification lateral branches grown on seedlings in the second time of grafting rejuvenation are cut to serve as scions to be grafted on stocks in the third time of selection. Half-lignification lateral branches grown on seedlings in the third time of grafting rejuvenation are cut as cuttage materials which are fast dipped into rooting agents and then are inserted into a seedbed for seedling exercising. According to the method for shoot cuttage rejuvenation of the superior adult trees of the pinus khasys, cuttage branches are obtained through the method that three times of grafting rejuvenation is conducted on the superior adult trees of the pinus khasys for the first time, and the defect that the branches of the superior adult trees of the pinus khasys cannot survive through direct cuttage. Cuttage propogation of the superior adult trees of the pinus khasys succeeds for the first time, and the rooting percentage is more than 62 percent.

The invention discloses a zelkova serrata field cutting propagation method which comprises the following steps of nursery land selection; soil and seedbed preparation; soil disinfection; cutting slip preparation; cuttage; management after field cuttage. According to field cuttage provided by the invention, by being compared with a facility cuttage, the processes of seedling exercising and transplanting are omitted, the cost for raising seedlings by cuttage is greatly reduced, so that the technology provided by the invention is more easy to master and popularize; in addition, the field cuttage method provided by the invention can stabilize the excellent characteristics of zelkova serrata through cutting propagation, and shortens a seedling period, nursery-grown plants grow uniformly, the requirements of forestation and afforesting on the quality and specification uniformity of the nursery-grown plants are met, the base parts of cuttings start to expand after cutting slips being cut for 30 days, adventitious roots generate at callused parts after one week, the rooting effect is good and the rate of survival reaches to 82 percent.

The fast reproduction process of Prunus mahaleb stock includes the preparation of tissue culture seedling, rooting culture of tissue culture seedling, seedling growth and hardening seeding, and transplanting. The tissue culture seedling and the rooting culture of tissue culture seedling are performed in a dark room; the seedling growth and hardening seeding is under natural lighting; and bud growing culture medium, multiplication culture medium and rooting culture medium suitable for the fast growth of Prunus mahaleb stock are used. The said process can produce robest stuggy Prunus mahaleb stock fast.

The invention discloses a method for quick propagation by means of hemp stem tip tissue culture, and belongs to the technical field of plant artificial cultivation. The method includes seed disinfection, germination culture, regeneration culture and rooting culture. Hormone-containing MS (Murashige and Skoog) culture media for the regeneration culture comprise sucrose with the concentration of 25-30 g / L and agar with the concentration of 7-8 g / L, the pH (potential of hydrogen) is 5.7-5.9, the culture temperatures are 23-25 DEG C, the illumination intensity is 1800-2300 lux, and the regeneration culture is carried out under 16h-illumination and 8h-dark conditions on a daily basis for 20-30 days. Hormone-containing MS culture media or hormone-containing 1 / 2 MS culture media for the rooting culture comprise sucrose with the concentration of 30 g / L and agar with the concentration of 8 g / L, the pH is 5.8, the culture temperatures are 23-25 DEG C, the illumination intensity is 2300 lux, andthe rooting culture is carried out under 16h-illumiantion and 8h-dark conditions on a daily basis for 20 days. The method has the advantages that regenerated plants can be quickly propagated by meansof stem tip tissue culture, and the method is short in experimental period.

The invention discloses a strawberry cutting seedling raising method. The strawberry cutting seedling raising method comprises the following steps: 1) selecting stolon seedlings with 2-3 expanded leaves; 2) soaking the lower ends of the stolon seedlings in fresh water for 10-12h and then soaking the lower ends of the stolon seedlings in growth regulating agent for 10-12h to obtain adventitious roots; 3) trimming a seedbed, punching holes in a ridge surface at distance with plant spacing of 20cm according to the standard of two rows per ridge, then inserting the adventitious roots in the holes, performing watering and keeping the ground temperature to be 10-20DEG C; 4) firstly erecting a sunshade net on the seedbed, simultaneously performing fertilization treatment, and keeping the temperature to be 20-25DEG C, illuminating time to be 10-12h and water holdup to be 70-80 percent. The strawberry cutting seedling raising method disclosed by the invention has the advantages that the steps are simple, the operation is convenient to perform, the cost is low, the performance of stock strawberries is effectively guaranteed to be excellent, the characteristics of short cultivation period, good rooting effect, quick seedling growth and high survival rate are realized, and the practice and popularization significance is great.

The invention provides a high-permeability light-weight seedling growing substrate for vegetable, belonging to the field of vegetable cultivation technology. The high-permeability light-weight seedling growing substrate comprises, by weight, 30-40 parts of perlite, 20-30 parts of vermiculite, 5-10 parts of fermented chicken manure, 10 parts of calcium magnesium phosphate fertilizer, 20-30 parts ofdouble-mushroom residues, 15-25 parts of peat, 40-50 parts of wood chips, 5-12 parts of traditional Chinese medicine residues, 10-20 parts of crushed vegetable straw and 3-5 parts of manganese sulfate. The seedling growing substrate for vegetable in the invention is specifically suitable for seedling raising of vegetable, and has the advantages of rich nutrients, good rooting properties and highsurvival rate.

The invention provides a solution capable of prompting rooting of taxus media. According to a formula, raffinose, saccharose and thiamine have the effects of promoting rooting of cottage plants; two traditional Chinese medicines, namely, astragalus membranaceus and liquorice are used in match with sodium humate, catechinic acid and citric acid to improve the effect of raffinose, saccharose and thiamine on promoting the rooting of the cottage plants, so that the rooting rate can be further increased, and the rooting rate of taxus media reaches more than 98%; meanwhile, a specific preparation process is performed to prepare the rooting solution, and the new roots of taxus media using the rooting solution are woody roots which are strong and high in environment adaptability and nutrition absorbency; as the woody roots increase, the resistance of seedling increases, and the survival rate of transplanting increases; in addition, the quantity of new tips is increased; leaves are the important places for the plants to synthesize nutrient; as the quantity of the new tips increases, more nutritional components can be synthesized by the plants, and therefore, the plants can be strong and strong in disease resistance.

The invention discloses a Crocus sativus L. tissue cultured corm strengthening and rooting medium and a tissue culture method. The provided medium employs an LS medium as a basic medium, 1L of the medium comprises 6-8g of carrageenan, 40-60 parts of white sugar, 0.1-0.5 part of active carbon, 20-40 parts of banana flesh, 10-30g of mashed potato, 0.1-1mg of naphthylacetic acid, 1-5mg of indolebutyric acid and 1-5mg of paclobutrazol, and the pH value is 5.8-6.0. The provided medium can promote strengthening and rooting of Crocus sativus L. tissue cultured corm, root warping is not easy, and the transplanting survival rate is raised. The tissue culture method comprises the following steps: firstly, Crocus sativus L. multiple shoots are taken and inoculated into an induction medium and a corm is obtained after induction culture; secondly, the corm is inoculated in the provided medium, and the corm is cultured until the diameter is not less than 10mm. The diameter of the Crocus sativus L. tissue cultured corm cultured through the method is 2.2 times of a diameter of the Crocus sativus L. corm cultured without the strengthening medium, the tissue cultured corm quality is raised, and the field growth cycle of corms is shortened.

The invention discloses a method for industrialized cutting seedling culture of Pteroceltis tatarinowii Maxim. According to the method, current-year branches of a tree 2-5 years old are selected as cuttings and sheared, so that the average length of the branches ranges from 10 cm to 12 cm, and the branches are planted in a cutting medium; the cuttings are planted and sufficiently watered, a powerful rooting raw powder solution with the concentration being 220-280 mg / L is sprayed to a seedbed with a sprayer, the sprayed solution covers the whole bed surface, and then, seedling management is performed. The technical requirement of the method is simple, Pteroceltis tatarinowii Maxim can be quickly bred in a large area, the cuttage survival rate of Pteroceltis tatarinowii Maxim reaches 93%, a basis is provided for industrialized seedling raising of Pteroceltis tatarinowii Maxim, and the method has great application and popularization value.

The invention relates to a cutting seedling-raising method of Pyrus betulifolia Bunge. The method comprises the following steps: step (1): preparing a cutting seedling bed; step (2): pretreating cuttings; step (3): performing cutting; step (4): performing management after cutting; and step (5): performing seedling-hardening and transplanting. The cuttings obtained by the method have good rooting effects, relatively many lateral roots, and good root system quality, and obtained seedlings have a high pass rate, and neat and consistent growth conditions, so that the cutting survival rate of Pyrusbetulifolia Bunge is effectively improved, and a solid foundation for an application of the cutting technology in the actual production of Pyrus betulifolia Bunge is laid.

The invention relates to cultivate medium composite, used for induce germ at bamboo. Wherein, it adds IAA 0.1-1.0mg / L or IBA 1.0mg / L into semi-density MS cultivate medium. The invention points out that growth adjuster IAA can accelerate the germ induction of bamboo. The invention also provides an application of said composite to cultivate the bamboo organism.

The invention relates to the technical field of novel cultivation, and provides a water culture cutting reproduction method of red maple. The method includes the steps: cutting collection and treatment: trimming young sprouts of the red maple into branches with double leaves and double buds to serve as cuttings, and cutting bases of the cuttings into inclined planes; culture solution preparation: blending water, sodium ethylene diamine tetraacetate, monopotassium phosphate, vitamin C, vitamin B12 and naphthylacetic acid to serve as culture solution; water culture: obliquely inserting the cuttings into the culture solution; daily management: controlling the temperature in a cutting room, the humidity in the cutting room and the temperature DEG C of the culture solution, regularly replacing the culture solution, and adding oxygen into the culture solution. The red maple is cultivated by the method, the rooting rate and the survival rate of cutting reproduction of the red maple can be remarkably increased, labor cost is reduced, seedlings can be cultured all the year round, and the method is applicable to mass production and factory production and has good application values and practical significance.

The invention provides a novel pseudo-ginseng seedling root biological coating agent, and a preparation method and applications thereof. Water soluble gel made of a water soluble film forming material, plant probiotics, and auxin is painted on the surface of the roots of pseudo-ginseng seedlings to form a layer of biological membrane with beneficial strains. The coating agent is prepared by the following steps: weighing a proper amount of water soluble film forming material, dissolving the film forming material by hot water to form a water solution (5-10%), cooling the water solution to a temperature of 28 to 30 DEG C, mixing the water soluble film forming material with an equal-volume water solution with a certain probiotics concentration, adding a root growth promoter into the mixed solution, and evenly mixing to obtain the pseudo-ginseng seedling root biological coating agent containing probiotics. The provided pseudo-ginseng seedling root biological coating agent has the advantages that the film forming performance and breathability are good, moreover, the biological coating agent carries a proper amount of beneficial microbes with rapid multiplication capacity, thus the biological coating agent can supply enough nutrients for the pseudo-ginseng seedlings and is capable of improving the micro-ecological environment in the late period of growth of pseudo-ginseng, and the yield of pseudo-ginseng is increased finally.

A plant rooting liquid and a preparing method thereof are disclosed. The plant rooting liquid is prepared from, by weight, 1-3 parts of an ABT rooting agent, 5-15 parts of glycine, 6-10 parts of leucine, 4-6 parts of lysine, 5-7 parts of phenylalanine, 12-14 parts of proline, 8-12 parts of aspartic acid, 1-3 parts of naphthaleneacetic acid, 0.8-1.2 parts of indolebutyric acid, 35-45 parts of white sugar, 15-25 parts of ethanol, 160-180 parts of water and 7-9 parts of monopotassium phosphate and zinc sulfate, with the weight ratio of the monopotassium phosphate to the zinc sulfate being 6-8:1. The rooting liquid can promote plant rooting, and can significantly increase the cottage survival rate when the plant rooting liquid is used for soaking plant branches difficult in rooting. The effect may be related to the weight ratio of the monopotassium phosphate to the zinc sulfate in raw materials. When the weight ratio of the monopotassium phosphate to the zinc sulfate is 6-8:1, the rooting promoting effect is best.

The invention provides a rooting agent containing a celery extract. The rooting agent comprises the preparation raw materials: the celery extract, oxalamide, sodium bicarbonate, an olive leaf extract, an onion extract, polylactic acid resin, super barium oxide, iron oxide black, a scaphium scaphigerum powder, magnesium oxide, a parthenocissus tricuspidata extract liquid, and a talcum powder. The invention also provides a preparation method of the rooting agent containing the celery extract; the method includes a base material preparation step, wherein the base material preparation step comprises the steps: stirring and mixing the rooting agent raw materials of the parthenocissus tricuspidata extract liquid, oxalamide and the talcum powder for 22-25 min. The rooting agent prepared by the method can be used for cuttage planting of pachira macrocarpa cutting slips in saline and alkaline land, can increase the callus rate of the pachira macrocarpa cutting slips, and can reduce the rot rate of the pachira macrocarpa cutting slips.

The invention provides a method for disinfecting and rapidly proliferating stems of aleurites montana. The method comprises the processes of explant disinfection, axillary bud induction, rooting culture and the like. The method provided by the invention overcomes the problems of a high pollution rate and difficult culture of primary culture of the stems of the aleurites montana, has the advantages of the low pollution rate of explants, the high propagation rate and a short culture period, provides a way for rapid propagation of excellent aseptic seedlings of the aleurites montana, and provides possibility for industrialized seedling production of the aleurites montana and subsequent transgenic technology.

The invention relates to agricultural rock wool for vegetable cultivation and seedling culture and belongs to the technical field of agricultural rock wool. The agricultural rock wool comprises a component A and a component B. The component A is prepared from, by weight, 90-95 parts of basalt, 3-4 parts of dolomite and 1-7 parts of silicon dioxide. The component B is prepared from, by weight, 80-90 parts of environment-friendly nontoxic resin, 300-360 parts of refined filtration water, 5-8 parts of a hydrophilic agent, 2-3 parts of a silane coupling agent and 8-10 parts of ammonium hydroxide. The agricultural rock wool has various buffering functions for soil cultivation, is very good in water absorbing quality, water retaining property and breathability, good in fiber forming effect, uniform in fiber distribution, soft and uniform in texture and beneficial to growth of the root systems of crops.

The invention provides a botanical rooting agent and a preparation method thereof. The botanical rooting agent comprises the following raw materials in parts by weight: 10-20 parts of a willow extract, 3-8 parts of a herba violae extract and 2-5 parts of a chlorophytum comosum extract. A preparation method of the botanical rooting agent comprises the following steps of: (1) uniformly mixing the willow extract, the herba violae extract and the chlorophytum comosum extract to obtain effective components; and (2) sequentially adding ethanol, fatty alcohol-polyoxyethylene ether, xanthan gum and glycerol into the effective components, adding water, and uniformly stirring to obtain the botanical rooting agent. The willow extract, the herba violae extract and the chlorophytum comosum extract areadopted as effective components of the rooting agent, rooting of crops can be effectively promoted, the waterlogging resistance of the crops can be improved, and due to the fact that the effective components are all plant extracts, the rooting agent is environmentally friendly, safe and high in environmental protection performance.

The invention discloses a tissue culture and rapid propagation method of angel red soft-seed pomegranate, and belongs to the field of plant tissue culture. The method comprises the following technologies: collecting tender stem segments, disinfecting with 75-percent alcohol for 30s,disinfecting with 0.1-percent mercury bichloride for 5 minutes and inoculating the disinfected stem segments on an adventitious bud inducing medium; after the adventitious buds grow out, cutting off two ends of leaves cutting off veins and inoculating to a leaf callus inducing medium; inoculating the adventitious buds produced by stem segment induction and leaf callus differentiation to a proliferation culture medium; transplanting single seedlings growing to 5 to 6 cm to a rooting medium and tempering the seedlings growing to 3 to 4 cm. The tissue culture and rapid propagation method disclosed by the invention has the advantage that rapid proliferation of angel red soft-seed pomegranate tube seedlings can be induced at an induction rate of 92.22 percent to produce a large amount of adventitious buds, and provides an effective way for industrialized seedling production.

The invention discloses a tissue culture and rapid propagation method of millettiadielsiana harms. The method comprises the following steps: selecting and treating of an explant, primary culture, differential culture, multiplication culture and rooting culture, thus forming a complete set of millettiadielsiana harms tissue culture and rapid propagation technology. The tissue culture and rapid propagation method has the characteristics of being short in culture period, high in multiplication coefficient, fast in multiplication speed, good in rooting effect, maintaining the excellent hereditary character of mother plant and the like, the factorized rapid seedling of the millettiadielsiana harms can be achieved, the multiplication coefficient can achieve 15.0-18.0, and compared with the tissue culture and rapid propagation of sterile seedlings obtained by taking seed sprouts, single buds and stems with buds as materials, the tissue culture and rapid propagation method is good in multiplication effect, high in multiplication efficiency, the stages of sprouting and growing of seeds into sterile seedlings in the culture media can be reduced, so that the culture period is shortened, thus providing a tissue culture and rapid propagation production technology for the medicinal and appreciable dual-use excellent material.