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Pseudo-ginseng seedling root biological coating agent, and preparation method and applications thereof

A coating agent and root growth technology, applied in botany equipment and methods, seed coating/seed dressing, chemicals for biological control, etc., can solve the biological coating of roots of Panax notoginseng species In order to achieve the effects of preventing diseases such as blight and root rot, avoiding skin damage, and reducing diseases such as blight and root rot

Active Publication Date: 2016-12-07
KUNMING INST OF BOTANY - CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] So far, the prior art has not seen the report of a kind of novel Panax notoginseng seedling root biological coating agent of the present invention

Method used

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  • Pseudo-ginseng seedling root biological coating agent, and preparation method and applications thereof
  • Pseudo-ginseng seedling root biological coating agent, and preparation method and applications thereof
  • Pseudo-ginseng seedling root biological coating agent, and preparation method and applications thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Accurately weigh 90g of polyvinylpyrrolidone (PVP), heat 1000mL of water, and stir to make it fully dissolved; Subculture 2-3 times under the condition of 25~28℃ to obtain well-growing colonies) of Chaetomium globosa (C.globosum) and Chaetomium chaeteum (C.cupreum) by conventional solid culture method In PDA medium (200g potatoes peeled, cut into 1cm 3 Small pieces, boiled for 20-30min, filtered, added 10g of glucose and 20g of agar to the filtrate, made the volume to 1L with pure water, and cultivated for 18 days, until the upper part of the petri dish was covered with spores, took out with a crystallization knife, and separated the two The fungi were formulated to a concentration of 2.0х10 5 500 mL of spore suspension per spore / mL; accurately weigh 300 mg of indole acetic acid IAA, a root growth promoter of Panax notoginseng, dissolve it with a small amount of ethanol, add water to make the volume to 200 mL, and prepare a mother liquor of IAA with a concentration of ...

Embodiment 2

[0031] Accurately weigh 70g of hydroxypropyl methylcellulose (HPMC), heat 1000mL of water, and stir to make it fully dissolved; Subculture 2-3 times under the conditions of 25~28℃ to obtain well-growing colonies) Streptomyces misionensis (S.misionensis) and A.vancoresmycina (A.vancoresmycina) were cultured on conventional solid Methods respectively in trehalose‐proline (S 2 ) solid medium (trehalose 5g, proline 1g, (NH4) 2 SO 4 1g, NaCl 1g, CaCl 2 2g,K 2 HPO 4 1g, MgSO 4 .7H 2 O 1g, multivitamins: riboflavin 1mg, niacin 1mg, calcium pantothenate 1mg, inositol 1mg, biotin 1mg, P‐aminobenzoic acid 1mg, VB 1 1mg, VB 6 1mg, 20g agar, water up to 1000mL, pH 7.2) and LB solid medium (yeast powder 5g, tryptone 8g, NaCl4g, agar 15g, pH 6.8) for 48h, until the colonies covered the entire culture dish, with a crystallization knife Take it out, prepare the concentration of Streptomyces rice repair (S.misionensis) and pseudomycobacterium (A.vancoresmycina) respectively to be ...

Embodiment 3

[0038] Precisely weigh 80g of sodium alginate, heat 1000mL of water, and stir to dissolve it fully; Carry out subculturing 2-3 times under the condition of ℃, obtain well-growth bacterium colony) chaetomium globus (C.globosum), horn chaetomium (C.cupreum) respectively in PDA medium (potato 200g peeled, cut into 1cm 3 Small pieces, boiled for 20‐30min, filtered, added 10g of glucose and 20g of agar to the filtrate, adjusted to 1L with purified water, and the pH was natural) and proliferated for 18 days until the upper part of the petri dish was covered with spores. The fungi were formulated to a concentration of 2.0х10 5 Each 500 mL of spore suspension per spore / mL; A. vancoresmycina (A. vancoresmycina) was propagated and cultivated in LB solid medium (5 g of yeast powder, 8 g of tryptone, 4 g of NaCl, 15 g of agar, pH 6.8) for 48 h. Colonies covered the entire Petri dish, and were taken out with a crystallization knife, and A.vancoresmycina was formulated to a concentration ...

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Abstract

The invention provides a novel pseudo-ginseng seedling root biological coating agent, and a preparation method and applications thereof. Water soluble gel made of a water soluble film forming material, plant probiotics, and auxin is painted on the surface of the roots of pseudo-ginseng seedlings to form a layer of biological membrane with beneficial strains. The coating agent is prepared by the following steps: weighing a proper amount of water soluble film forming material, dissolving the film forming material by hot water to form a water solution (5-10%), cooling the water solution to a temperature of 28 to 30 DEG C, mixing the water soluble film forming material with an equal-volume water solution with a certain probiotics concentration, adding a root growth promoter into the mixed solution, and evenly mixing to obtain the pseudo-ginseng seedling root biological coating agent containing probiotics. The provided pseudo-ginseng seedling root biological coating agent has the advantages that the film forming performance and breathability are good, moreover, the biological coating agent carries a proper amount of beneficial microbes with rapid multiplication capacity, thus the biological coating agent can supply enough nutrients for the pseudo-ginseng seedlings and is capable of improving the micro-ecological environment in the late period of growth of pseudo-ginseng, and the yield of pseudo-ginseng is increased finally.

Description

technical field [0001] The invention relates to the technical field of plant protection, in particular to a novel notoginseng seedling root biological coating agent and a preparation method and application thereof. Background technique [0002] Panax notoginseng (Burk.) F.H.Chen is a plant of the genus Ginseng of Araliaceae, which is mainly produced in Wenshan, Yunnan. It is a precious traditional Chinese medicine in China and an important characteristic herbal medicine in Yunnan Province. Since the 1980s, with the continuous elucidation of the active ingredients and pharmacological effects of Panax notoginseng, the products of Panax notoginseng have been continuously developed, and the market demand has increased day by day. In recent years, with the expansion of the scale of the Panax notoginseng industry, the problem of continuous cropping obstacles in the Panax notoginseng planting industry is very prominent. Continuous cropping caused a large-scale outbreak of root dis...

Claims

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Application Information

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IPC IPC(8): A01N63/04A01N63/02A01N43/38A01P21/00A01C1/06
CPCA01C1/06A01N43/38A01N63/10A01N63/30A01N2300/00
Inventor 朱宏涛张颖君王东杨崇仁
Owner KUNMING INST OF BOTANY - CHINESE ACAD OF SCI
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